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qPCR Array System from Bioneer

Reference gene panel (Human, Mouse, Rat)

Overview

Human Reference gene : Validated Real-Time PCR Primer Library

Human validated Real-Time PCR Primer Library는 Human genome을 대상으로 bioinformatics tool을 이용하여 제작한 후 모든 primer들의 증폭 효율을 Exicycler™96과 Accupower® Greenstar™ qPCR PreMix를 이용하여 검증하였습니다.

Human reference gene information
# Symbol Accession No. Description Primer No.
1 ACTB NM_001101.3 Actin, beta PH00001
2 B2M NM_004048 Beta-2-microglobulin P110309
3 GAPDH NM_002046 Glyceraldehyde-3-phosphate dehydrogenase P267613
4 HMBS NM_000190 Hydroxymethylbilane synthase P251739
5 HPRT1 NM_000194 Hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome) P160523
6 PGK1 NM_000291 Glucuronidase, beta P326829
7 TBP NM_003194 TATA box binding protein P121995
8 TFR2 NM_003227 Transferrin receptor 2 PH00003
9 TFRC NM_003234 Transferrin receptor (p90, CD71) P304167
10 TUBB NM_178014 Tubulin, beta P277069
11 UBC NM_021009 Ubiquitin C PH00002
12 YWHAZ NM_145690,NM_003406 Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein,zeta polypeptide P211166
13 18SrRNA NR_003286.2 Human 18S ribosomal RNA PH00004
14 GUSB NM_000181 Glucuronidase, beta PH00005
15 HSP90AB1 NM_007355 Heat shock protein 90kDa alpha (cytosolic), class B member 1 PH00006
16 MRPL19 NM_014763 Mitochondrial ribosomal protein L19 PH00007
17 PPIA NM_021130 Peptidylprolyl isomerase A (cyclophilin A) PH00008
18 PSMC4 NM_006503.2 Proteasome (prosome, macropain) 26S subunit, ATPase, 4 PH00009
19 PUM1 NM_014676 Pumilio homolog 1 (Drosophila) PH00010
20 RPL13A NM_012423 Ribosomal protein L13a PH00011
21 RPLP0 NM_001002 Ribosomal protein, large, P0 PH00012

Primer testing & validation : Human reference gene


Mouse Reference gene : Validated Real-Time PCR Primer Library

Mouse validated Real-Time PCR Primer Library는 Mouse genome을 대상으로 bioinformatics tool을 이용하여 제작한 후 모든 primer들의 증폭 효율을 Exicycler™96과 Accupower® Greenstar™ qPCR PreMix를 이용하여 검증하였습니다.

Mouse reference gene information
# Symbol Accession No. Description Primer No.
1 18SrRNA NR_003278.1 18S RNA Ribosomal RNA PM00001
2 Actb NM_007393.3 Actin, beta, cytoplasmic PM00002
3 B2m NM_009735.3 Beta-2 microglobulin PM00003
4 Gapdh NM_008084.2 Glyceraldehyde-3-phosphate dehydrogenase PM00004
5 Gusb NM_010368.1 Glucuronidase, beta PM00005
6 Hmbs NM_013551.2 Hydroxymethyl-bilane synthase Heme synthesis, porphyrin metabolism PM00006
7 Hprt1 NM_013556.2 Hypoxanthine guanine phosphoribosyl transferase PM00007
8 Hsp90ab1 NM_008302.3 Heat shock protein 90 alpha (cytosolic), class B member 1 PM00008
9 Ldhal6b NM_175349.2 Lactate dehydrogenase A-like 6B PM00009
10 Pgk1 NM_008828.2 phosphoglycerate kinase 1 PM00010
11 Ppia NM_008907.1 Peptidylprolyl isomerase A PM00011
12 Tbp NM_013684.3 TATA box binding protein PM00012

Primer testing & validation : Mouse reference gene


Rat Reference gene : Validated Real-Time PCR Primer Library

Rat validated Real-Time PCR Primer Library는 Rat genome을 대상으로 bioinformatics tool을 이용하여 제작한 후 모든 primer들의 증폭 효율을 Exicycler™96과 Accupower® Greenstar™ qPCR PreMix를 이용하여 검증하였습니다.

Rat reference gene information
# Symbol Accession No. Description Primer No.
1 Actb  NM_031144  Actin, beta PR00001
2 B2m  NM_012512  Beta-2 microglobulin PR00002
3 Gusb NM_017015  Glucuronidase, beta, b PR00003
4 Hprt1 NM_012583  Hypoxanthine guanine phosphoribosyl transferase PR00004
5 Hspcb(Hsp90ab1)  NM_001004082  Heat shock 90kDa protein 1, beta PR00005
6 Ldha  NM_017025  Lactate dehydrogenase A PR00006
7 Pgk1  NM_053291  Phosphoglycerate kinase 1 PR00007
8 Ppih  XM_345576  Peptidylprolyl isomerase H (cyclophilin H) PR00008
9 Rpl13a  NM_173340  Ribosomal protein L13A PR00009
10 Rplp1  NM_001007604  Ribosomal protein, large, P1 PR00010
11 Sdha  NM_130428  Succinate dehydrogenase complex, subunit A, flavoprotein (Fp) PR00011
12 Tfrc  XM_340999  Transferrin receptor PR00012

Primer testing & validation : Rat reference gene