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1Genomic DNA from cultured mammalian cell (HeLa)
- Sample volume: 1X106 cells
- Protocol number: 109
- Total prep time: about 1 hr 30 min.
- Average yield: 4-8 μg
• Agarose gel analysis
Figure 1. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X106 cells of cultured HeLa cell and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.8 and 2.0 (not shown).
2Genomic DNA from bacteria (E.coli)
- Sample volume: 1X109 cells
- Protocol number: 109
- Total prep time: about 1 hr 40 min.
- Average yield: 8-12 µg
• Agarose gel analysis
Figure 2. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 1X109 cells of E.coli cells and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).
3Genomic DNA from tissue (bovine skeletal muscle)
- Sample volume: ~ 25 mg
- Protocol number: 102
- Total prep time: about 1 hr 30 min.
- Average yield: Up to 20 µg
• Agarose gel analysis
Figure 3. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 25 mg of bovine tissues and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).
4Genomic DNA from plant tissue (spinach leaf)
- Sample volume: ~ 100 mg
- Protocol number: 104
- Total prep time: about 1 hr
- Average yield: Up to 5 µg
• Agarose gel analysis
Figure 4. Lanes 1, 3, 5, 7, 9, 11, 13, 15 were extracted with 100 mg of spinach leaves and lanes 2, 4, 6, 8, 10, 12, 14, 16 were extracted with ddH2O as a negative control in DNA extraction. Note all samples have very similar yields. Purity was also tested and was consistently between 1.9 and 2.0 (not shown).
5RNA extraction from cultured mammalian cells (HeLa)
- Sample volume: 1.0 X 106 cells
- Protocol number: 207
- Average yield: Up to 10 µg
- Average RQS: 9.5 이상
• Electrophoresis result with LabChip GX
Figure 5. Results of capillary electrophoresis of total RNA extracted from HeLa cell (1X106 cells) using LabChip GX (Caliper life science, USA). Average RQS (RNA Quality Score) were at least 9.5 shown.
• Real-time RT-PCR results of total RNA extracted from cultured cell
Figure 6. Real-time RT-PCR results using an AccuPower® RocketScrpt ™ RT PreMix (K-2101, Bioneer)와 AccuPower® Dualstar ™ qPCR PreMix (K-6110, Bioneer) and run on Exicycler ™ 96 Real-Time PCR System (A-2060, Bioneer) from total RNA extracted from HeLa cell (1X106 cells)
