Taq DNA Polymerase

Taq DNA Polymerase 는 Thermus aquaticus YT1 유래 DNA polymerase gene을 재조합 기술을 이용하여 Escherichia coli로부터 발현시켜 순수 분리 정제된 94kDa의 내열성 Thermus aquaticus DNA polymerase입니다. 

₩65,000
카탈로그 번호
E-2011-CFG

Features and Benefits

High Yields & High Sensitivity:
높은 증폭 효율과 template에 대한 높은 민감도를 갖습니다.

Robust Performance:
Taq enzyme에 최적화된 버퍼를 제공하여 안정적인 PCR 반응을 수행합니다.

Reproducibility:
재현성 있는 결과를 위해 바이오니아의 전 제품은 one batch에서 대량생산되고 엄격한 ISO 품질 검사를 통해 항상 균일한 품질을 보증합니다.

Specifications

5' to 3' exonuclease activity : Yes
3' to 5' exonuclease activity : No
3' – A overhang : Yes
Fragment size: Up to 10 kb

Applications

Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye
Gene expression profiling
Microbial & viral pathogen detection

Reagents Supplied

Component

E-2011 E-2011-1 E-2011-2 E-2011-3
Taq Plus DNA polymerase (500 U) 100 μl 100 μl 100 μl 100 μl
Dilution buffer 1 ml 1 ml 1 ml 1 ml
10X Reaction buffer 1 ml 1 ml 1 ml 1 ml
20 mM MgCl2   1ml   1ml
10 mM dNTP 1 ml 1 ml    

10X reaction buffer with (or without) MgCl2: 100 mM Tris-HCl, 400 mM KCl, 15 mM MgCl2, pH 9.0
Dilution buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50 % Glycerol, pH 8.0
dNTP mix: 2.5 mM of each dNTP

Concentration
500 units (5 U/μl)

Storage Conditions
20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50 % Glycerol pH 8.0

Store Temperature
-20°C

Unit Definition
One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.

Experimental data


Figure 1. Taq DNA Polymerase의 sensitivity test
Taq DNA Polymerase 1 unit으로 DNA Polymerase의 활성을 확인한 실험으로 human genomic DNA (A)와 Lambda DNA (B)를 이용한 실험 결과이며 각 template DNA는 100 ng부터 100 fg까지 희석하여 실험.

M; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040,)
Lane 1; 100 ng template DNA
Lane 2; 10 ng template DNA
Lane 3; 1 ng template DNA
Lane 4; 100 pg template DNA
Lane 5; 10 pg template DNA
Lane 6; 1 pg template DNA
Lane 7; 100 fg template DNA



Figure 2. Taq DNA polymerase serial dilution test
Taq DNA Polymerase를 1/5, 1/10, 1/15, 1/20로 희석하여 human genomic DNA (A), Lambda DNA (B) 를 template로 하여 PCR 반응.

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1; 5 U of Taq DNA polymerase
Lane 2; 1 U of Taq DNA polymerase
Lane 3; 0.5 U of Taq DNA polymerase
Lane 4; 0.33 U of Taq DNA polymerase
Lane 5; 0.25 U of Taq DNA polymerase

Figure 3. Taq DNA Polymerase의 long kb DNA 증폭 실험
5 kb 부터 8 kb 까지 Lambda DNA 20 pg을 template로 이용하여 증폭 test.

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1; 5 kb PCR product
Lane 2; 6 kb PCR product
Lane 3; 7 kb PCR product
Lane 4; 8 kb PCR product



Cat. No. Product Description Price
E-2011 Taq DNA Polymerase, 500 U, 10 mM dNTP, 10X Reaction Buffer with MgCl2 ₩90,000
E-2011-1 Taq DNA Polymerase, 500 U, 10 mM dNTP, 10X Reaction Buffer, 20 mM MgCl2 ₩90,000
E-2011-2 Taq DNA Polymerase, 500 U, 10X Reaction Buffer with MgCl2 ₩65,000
E-2011-3 Taq DNA Polymerase, 500 U, 10X Reaction Buffer, 20 mM MgCl2 ₩65,000
E-2013 Taq DNA Polymerase, 2,000 U, 10 mM dNTP, 10X Reaction Buffer with MgCl2 ₩315,000
E-2013-1 Taq DNA Polymerase, 2,000 U, 10 mM dNTP, 10X Reaction Buffer, 20 mM MgCl2 ₩315,000
E-2013-2 Taq DNA Polymerase, 2,000 U, 10X Reaction Buffer with MgCl2 ₩227,000
E-2013-3 Taq DNA Polymerase, 2,000 U, 10X Reaction Buffer, 20 mM MgCl2 ₩227,000











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