ProFi Taq DNA Polymerase

ProFi Taq DNA polymerase는 Taq DNA polymerase를 개선한 효소로써 high effiency 및 long-range PCR에 매우 적합한 제품입니다. complex genomic DNA 또는 cDNA templates,low-copy targets등 다양한 PCR 증폭반응에서 사용할 수 있는 제품입니다.

₩100,000
카탈로그 번호
E-2201-CFG

특장점

  • 높은 민감도

    Lambda DNA 및 human gDNA를 template로 사용했을 때 타사 제품보다 높은 증폭 효율 및 민감도를 가집니다.

  • Long Range PCR

    Lambda DNA의 경우 30 kb, human gDNA의 경우 21 kb의 PCR 산물을 효과적으로 증폭시킬 수 있습니다.

  • 재현성

    ISO 9001 품질시스템 하에서 생산되어 각 batch에 대한 균일한 품질의 제품이 공급되기 때문에 재현성있는 결과를 얻을 수 있습니다.

응용 및 적용

  • Primer extension
  • Long-range amplification from genomic DNA
  • High amplification efficiency
  • Excellent performance on difficult template
  • Amplification of low-copy targets
  • High yield and high sensitivity PCR

실험 자료

Figure. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reaction using other suppliers' DNA polymerase were performed according to each supplier's protocol.


Target: human insulin receptor gene
Lane M; 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1; 10 ng of human genomic DNA
Lane 2; 1 ng of human genomic DNA
Lane 3; 100 pg of human genomic DNA
Lane 4; 10 pg of human genomic DNA

제품 규격/사양

5' to 3' exonuclease activity

Yes

3' to 5' exonuclease activity

Yes

3' – A overhang

Yes

PCR product size

Up to 30 kb

제품 구성

10X reaction buffer with (or without) MgCl2 : Tris-HCl, KCl, 15 mM MgCl2, pH 9.0

Dilution buffer : 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stabilizers, 50 % Glycerol, pH8.0

10 mM dNTPs mix: 2.5 mM of each dNTP

농도

250 Units (5 U/μl)

저장 조건

20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0

보관 온도

-20℃

실험 자료

Figure 1. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase. 
cDNA synthesized from 10-fold serial-diluted human total RNA from 10 ng to 10 pg using AccuPower® RocketScript™ Cycle RT PreMix (Bioneer, Cat. No. K-2201) was used as a template for PCR amplification. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 33 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target: human GAPDH gene
Lane M; 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1; 10 ng of human total cDNA
Lane 2; 1 ng of human total cDNA
Lane 3; 100 pg of human total cDNA
Lane 4; 10 pg of human total cDNA



Figure 2. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec and 68°C for 4 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Lane M; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 2 kb fragment (human tumor protein p53 gene)
Lane 2; 3 kb fragment (human tumor protein p53 gene)
Lane 3; 4.5 kb fragment (human DNA cross-link repair 1A gene)
Lane 4; 8 kb fragment (human hemoglobin epsilon 1 gene




Figure 3. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec and 68°C for 15 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Human genomic DNA was used as a template for PCR amplification.

Lane M1; Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 11 kb fragment
Lane 2; 13.5 kb fragment
Lane 3; 17.6 kb fragment
Lane 4; 21.4 kb fragment




Figure 4. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec, 65°C for40 sec, and 68°C for 20 min.
PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Lambda DNA was used as a template for PCR amplification.

Lane M1; Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 15 kb fragment
Lane 2; 20 kb fragment
Lane 3; 25 kb fragment
Lane 4; 30 kb fragment


카탈로그 번호 제품명 가격
E-2201 ProFi Taq DNA Polymerase 250 U, 10 mM dNTPs, 10X reaction buffer with MgCl2 ₩120,000
E-2202 ProFi Taq DNA Polymerase 250 U, 10 mM dNTPs, 10X reaction buffer without MgCl2, 20 mM MgCl2 ₩120,000
E-2203 ProFi Taq DNA Polymerase 250 U, 10X reaction buffer with MgCl2 ₩100,000
E-2204 ProFi Taq DNA Polymerase 250 U, 10X reaction buffer without MgCl2, 20 mM MgCl2 ₩100,000
E-2205 ProFi Taq DNA Polymerase 1000 U, 10 mM dNTPs, 10X reaction buffer with MgCl2 ₩420,000
E-2206 ProFi Taq DNA Polymerase 1000 U, 10 mM dNTPs, 10X reaction buffer without MgCl2, 20 mM MgCl2 ₩420,000
E-2207 ProFi Taq DNA Polymerase 1000 U, 10X reaction buffer with MgCl2 ₩350,000
E-2208 ProFi Taq DNA Polymerase 1000 U, 10X reaction buffer without MgCl2, 20 mM MgCl2 ₩350,000
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