AccuPower® PCR PreMix & Master Mix

AccuPower® PCR PreMix 는 바이오니아의 특허 기술인 DNA polymerase, dNTPs, reaction buffer 등 PCR 수행에 필요한 구성성분을 혼합하여 1 회 분량씩 진공 건조 시킨 제품입니다. 또한 안정화 물질이 첨가되어 있어 상온 (25°C) 보관할 경우에는 한 달 동안, 냉동 보관 할 경우에는 2년 동안 활성이 안정적으로 유지됩니다.

Grouped product items
상품명 수량
AccuPower® PCR PreMix (96 T, 20 μl)
₩54,000
AccuPower® PCR PreMix (96 T, 50 μl)
₩81,000
AccuPower® PCR PreMix (480 T, 20 μl)
₩199,000
AccuPower® PCR PreMix (-dye) (480 T, 20 μl)
₩199,000
AccuPower® PCR PreMix (480 T, 50 μl)
₩304,000
AccuPower® PCR Master Mix (2X, 1 ml)
₩48,000
AccuPower® PCR Master Mix (2X, 10 ml)
₩317,000

특장점

  • 안정성

    AccuPower® PCR PreMix는 PCR 반응 혼합액에 안정화 물질이 첨가되어 있어 상온배송이 가능하고 solution type 제품보다 안정성이 뛰어납니다.

  • 편리성

    각 PCR tube에 1회 반응씩 PCR 반응물이 건조되어 있어 누구나 손쉽게 template DNA 및 primer 만을 첨가하여 PCR 반응을 수행할 수 있습니다.

  • 민감도

    미량의 DNA도 뛰어난 민감도와 증폭 효율로 PCR 반응이 가능합니다.

  • 재현성

    ISO 9001 품질 시스템 하에서 대량 생산되어 lot 간 편차가 최소화 되었으며, 반복적인 실험에도 재현성 있는 실험 결과를 제공합니다.

응용 및 적용

• Conventional PCR

• Primer extension

• TA cloning

• Gene sequencing

실험 자료


premix figure2

Figure. Comparison of heat-stability of AccuPower® PCR PreMix

AccuPower® PCR PreMix is incubated at 95℃ with various time.
Kit: AccuPower® PCR PreMix (Batch-1/2)

A; 30 min, B; 60 min, C; 90 min

Lane 1; Human genomic DNA 10 ng
Lane 2; Human genomic DNA 1 ng
Lane 3; Human genomic DNA 100 pg
Lane 4; Human genomic DNA 10 pg
M ;100 bp plus DNA ladder (Bioneer Cat. No: D-1035)

제품 규격/사양

Enzyme

Top DNA polymerase

5' to 3' exonuclease activity

No

3' to 5' exonuclease activity

No

3' – A overhang

Yes

Fragment Size

Up to 10 kb

실험 자료

premix figure1

Figure 1. High sensitivity of AccuPower® PCR PreMix

Lane 1; Lamda DNA 10 ng Lane 2; Lamda DNA 1 ng
Lane 3; Lamda DNA 100 pg Lane 4; Lamda DNA 10 pg
Lane 5; Lamda DNA 1 pg Lane 6; Lamda DNA 100 fg
Lane 7; Lamda DNA 10 fg Lane 8; Lamda DNA 1 fg
Lane 9; Human genomic DNA 10 ng Lane 10; Human genomic DNA 1 ng
Lane 11; Human genomic DNA 100 pg Lane 12; Human genomic DNA 10 pg
M; 100 bp plus DNA ladder (Bioneer Cat. No: D-1035)

premix figure3

Figure 2.Comparison of PCR amplification sensitivity between AccuPower® PCR PreMix from Bioneer and other suppliers‘ PCR kit.

Lane 1; Lamda DNA 10 ng
Lane 2; Lamda DNA 1 ng
Lane 3; Lamda DNA 100 pg
Lane 4; Lamda DNA 10 pg
Lane 5; Lamda DNA 1 pg
Lane 6; Lamda DNA 100 fg
Lane 7; Lamda DNA 10 fg
Lane 8; Lamda DNA 1 fg


premix figure4

Figure 3. Comparison of PCR amplification of long targets between AccuPower® PCR PreMix from Bioneer and other supplier's PCR kit.

A; 5 kb B; 6 kb C; 7 kb

Manual

Manual_AccuPower® PCR PreMix

Manual_AccuPower® PCR Master Mix

Manual_AccuPower® PCR PreMix (Negative Dye) 


MSDS

MSDS_AccuPower® PCR PreMixs


Brochure

All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate


카탈로그 번호 제품명 가격
K-2011 AccuPower® PCR PreMix, 0.5 mL thin wall microtube / 100 tubes, 50 μl reaction ₩95,000
K-2012 AccuPower® PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes, 20 µl reaction ₩54,000
K-2013 AccuPower® PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes, 50 µl reaction ₩81,000
K-2016 AccuPower® PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes, 20 µl reaction ₩199,000
K-2017 AccuPower® PCR PreMix, 0.2 mL thin wall 8-strip tubes with individual caps / 480 tubes, 50 μl reaction ₩304,000
K-2018 AccuPower® PCR Master Mix, 1 ml of 2 X Master mix solution ₩48,000
K-2018-1 AccuPower® PCR Master Mix, 10 ml of 2 X Master mix solution ₩317,000
K-2080-1 AccuPower® PCR PreMix, thin-wall 384-well full-skirted plate, 5 µl reaction ₩216,000
K-2080-2 AccuPower® PCR PreMix, thin-wall 384-well full-skirted plate, 10 µl reaction ₩230,000
K-2080-3 AccuPower® PCR PreMix, thin-wall 384-well full-skirted plate, 20 µl reaction ₩243,000
K-2260-1 AccuPower® PCR PreMix, thin-wall 96-well flat plate, 10 µl reaction ₩54,000
K-2260-2 AccuPower® PCR PreMix, thin-wall 96-well full-skirted plate, 10 µl reaction ₩54,000
K-2260-3 AccuPower® PCR PreMix, thin-wall 96-well semi-skirted plate, 10 µl reaction ₩54,000
K-2260-4 AccuPower® PCR PreMix, thin-wall 96-well flat plate, 20 µl reaction ₩59,000
K-2260-5 AccuPower® PCR PreMix, thin-wall 96-well full-skirted plate, 20 µl reaction ₩59,000
K-2260-6 AccuPower® PCR PreMix, thin-wall 96-well semi-skirted plate, 20 µl reaction ₩59,000
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