AccuPower® HotStart Pfu PCR PreMix

AccuPower® HotStart Pfu PCR PreMix는 바이오니아의 enzyme-mediated HotStart 특허기술을 적용하여 반응 특이성과 PCR 증폭효율을 높인 제품으로, Pfu DNA polymerase가 낮은 온도에서 반응하면서 발생할 수 있는 mis-priming, primer-dimer와 같은 비특이 반응을 줄여줍니다. 또한 Pfu DNA polymerase는 proof-reading (3'→5' exonuclease) 기능이 있어서 DNA 증폭 반응 시 발생하는 error를 줄일 수 있어 단백질 생산을 위한 cloning 실험에 적합합니다.

₩97,000
카탈로그 번호
K-2301-CFG

특장점

  • 높은 정확성

    높은 정확성 (error rate= 1.9X10-6)을 가지고 있어 DNA 증폭 시 발생하는 mutation을 최소화하였습니다.

    figure2

  • 높은 특이도

    Pfu DNA polymerase의 proof-reading 기능에 PyroHotStart 기술을 적용하여 cloning, mutagenesis 등 정확한 sequence가 요구되는 실험을 PCR Purification 정제만으로도 가능합니다.

  • 편리성

    Template DNA 와 primer 만을 첨가하여 PCR 반응을 누구나 손쉽게 사용할 수 있으며, Hotstart 기능으로 정확한 타겟만을 증폭 시킴으로써 Cloning 등의 실험 시 Gel extraction 하는 번거로움을 최소화 하였습니다.

  • 안정성

    PCR 반응 혼합액에 안정화제가 포함되어 있어 상온배송이 가능하고 solution type 제품보다 안정성 뛰어납니다.

  • 재현성

    ISO 9001 품질 시스템 하에서 대량 생산되어 lot 간 편차가 최소화 되었으며, 반복적인 실험에도 재현성 있는 실험 결과를 제공합니다.

응용 및 적용

• Gene cloning with blunt ends

• Site-directed mutagenesis

• High fidelity amplification

• High specificity PCR

• cDNA amplification

실험 자료

accupower

Figure. Comparison of PCR amplification efficiency between AccuPower® Hotstart Pfu PCR PreMix from Bioneer and other suppliers' PCR master mix
Target : human insulin receptor gene. The cycling conditions for AccuPower® Hotstart Pfu PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec and 72°C for 2min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.

Lane M ; 100 bp DNA Ladder(Cat. No. D-1030,Bioneer)
Lane 1 ; 10 ng of human genomic DNA
Lane 2 ; 1 ng of human genomic DNA
Lane 3 ; 100 pg of human genomic DNA
Lane 4 ; 10 pg of human genomic DNA

제품 규격/사양

Enzyme

Pfu DNA polymerase

5' to 3' exonuclease activity

No

3' to 5' exonuclease activity

Yes

3'- A overhang

No

Fragment Size

Up to 5 kb

실험 자료

accupower

Figure 1. AccuPower® Hotstart Pfu PCR PreMix shows enhanced specificity compared to competitors.
Specificity test was performed using 7 different sets of primers targeting the P53 gene. 10 ng of human genomic DNA was used for each PCR reaction. The cycling conditions were 95°C for 5min, 32 cycles of 95°C for 30 sec, 62°C for 40 sec, and 72°C for 1 min 30sec, and 72°C for 5 min for final extension.

Lane M; 100 bp DNA Ladder (Cat. No. D-1030,Bioneer)
Lane 1; P75/73 primer set (139 bp)
Lane 2; P55/53 primer set (211 bp)
Lane 3; P55/63 primer set (447 bp)
Lane 4; P75/83 primer set (618 bp)
Lane 5; P55/73 primer set1082 bp)
Lane 6; P65/83 primer set (1296 bp)
Lane 7; P55/83 primer set (1561 bp)




accupower

Figure 2. Comparison of PCR amplification specificity between AccuPower® Hotstart Pfu PCR PreMix from Bioneer and other suppliers' pfu DNA polymerase.
Three different primer-template systems were amplified under the same conditions. The cycling conditions were 95°C for 5min, 35 cycles of 95°C for 30 sec, 62°C for 30 sec, and 72°C for 1 min 30 sec, and 72°C for 5 min for final extension.This data shows that AccuPower® Hotstart Pfu PCR PreMix has higher amplificition efficency and specificity than other suppliers' pfu DNA polymerase.

Lane M; 100 bp DNA Ladder (Cat. No. D-1030,Bioneer) 
Lane 1; 100 ng DNA, ApoE primer set (268 bp)
Lane 2; 10 ng DNA, ApoE primer set (268 bp)
Lane 3; 100 ng DNA, PrP primer set (500 bp)
Lane 4; 10 ng DNA, PrP primer set (500 bp)
Lane 5; 100 ng DNA, PrP primer set (705 bp)
Lane 6; 10 ng DNA, PrP primer set (705 bp)




accupower

Figure 3.AccuPower® Hotstart Pfu PCR PreMix has high amplification efficiency.
Bioneer reaction mixture was followed by 95°C for 5 min, 35 cycles of 95°C for 20 sec, 65°C for 20 sec, and 68°C for 15 min , and 68°C for 5 min for final extension.

Lane M; 1 kb DNA Ladder (Cat. No. D-1040,Bioneer) 
Lane 1; 2 kb fragment
Lane 2; 2.5 kb fragment
Lane 3; 3 kb fragment
Lane 4; 4.5 kb fragment
Lane 5; 5 kb fragment

Manual

Manual_AccuPower® HotStart Pfu PCR PreMix


MSDS

MSDS_AccuPower® Hotstart Pfu PCR PreMix


Brochure

AccuPower® PyroHotstart Taq PCR PreMix

AccuPower® HotStart Pfu PCR PreMix_Flyer

All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate

카탈로그 번호 제품명 가격
K-2301 AccuPower® HotStart Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps /96 tubes, 20 μl reaction ₩97,000
K-2302 AccuPower® HotStart Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps /96 tubes, 50 μl reaction ₩146,000
K-2303 AccuPower® HotStart Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps /480 tubes,20 μl reaction ₩364,000
K-2304 AccuPower® HotStart Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps /480 tubes, 50 μl reaction ₩546,000
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