AccuPower® HotStart Pfu PCR PreMix

AccuPower® HotStart Pfu PCR PreMix는 바이오니아의 enzyme-mediated HotStart 특허기술을 적용하여 반응 특이성과 PCR 증폭효율을 높인 제품으로, Pfu DNA polymerase가 낮은 온도에서 반응하면서 발생할 수 있는 mis-priming, primer-dimer와 같은 비특이 반응을 줄여줍니다. 또한 Pfu DNA polymerase는 proof-reading (3'→5' exonuclease) 기능이 있어서 DNA 증폭 반응 시 발생하는 error를 줄일 수 있어 단백질 생산을 위한 cloning 실험에 적합합니다.

₩97,000
In stock
SKU
K-2301-CFG
Features and Benefits

High fidelity:  
Proof-reading 기능과 높은 특이성을 가지고 있어 cloning에 가장 적합한 제품입니다. 


figure2

High specificity: 
Pfu DNA polymerase의 proof-reading 기능과 enzyme-mediated HotStart 기술을 통해 cloning, mutagenesis 등 정확한 sequence가 요구되는 실험에서 최적의 PCR product를 얻을 수 있습니다.

figure1

Ease-of-Use: 
각각의 PCR tube에 DNA polymerase와 PCR 수행에 필요한 모든 구성 성분이 포함되어 있어 template DNA, primer set, D.W.만 넣어 바로 PCR 반응을 수행할 수 있습니다. 또한 전기 영동 시에 필요한 tracking dye 와 침강제가 포함되어 있어 sample loading buffer를 추가로 넣을 필요가 없으므로 간편하게 사용할 수 있습니다. 

Stability: 
PCR 반응 혼합물에 안정화제를 첨가하여 건조시켜, 장기간 보관하더라도 활성을 안정적으로 유지합니다. 

Reproducibility : 
ISO 9001 품질 시스템 하에서 one-batch system으로 대량 생산되어 각 batch에 대한 철저한 QC를 거친 후 균일한 품질의 제품으로 공급되기 때문에 사용자가 대량의 시료를 반복적으로 처리할 때 각 tube마다 발생하는 편차 문제를 해결하여 재현성있는 실험 결과를 제공합니다.

Specifications

Enzyme: Pfu DNA polymerase
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: Yes
3' – A overhang: No
Fragment Size: Up to 5 kb


Applications

• Gene cloning with blunt ends
• Site-directed mutagenesis
• High fidelity amplification
• High specificity PCR
• cDNA amplification

Experimental Data

accupower
Figure 1. Comparison of PCR amplification efficiency between AccuPower® Hotstart Pfu PCR PreMix from Bioneer and other suppliers' PCR master mix
Target : human insulin receptor gene. The cycling conditions for AccuPower® Hotstart Pfu PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec and 72°C for 2min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.
Lane M ; 100 bp DNA Ladder(Cat. No. D-1030,Bioneer)
Lane 1 ; 10 ng of human genomic DNA
Lane 2 ; 1 ng of human genomic DNA
Lane 3 ; 100 pg of human genomic DNA
Lane 4 ; 10 pg of human genomic DNA


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Figure 2. AccuPower® Hotstart Pfu PCR PreMix shows enhanced specificity compared to competitors.
Specificity test was performed using 7 different sets of primers targeting the P53 gene. 10 ng of human genomic DNA was used for each PCR reaction. The cycling conditions were 95°C for 5min, 32 cycles of 95°C for 30 sec, 62°C for 40 sec, and 72°C for 1 min 30sec, and 72°C for 5 min for final extension.
Lane M; 100 bp DNA Ladder (Cat. No. D-1030,Bioneer) 
Lane 1; P75/73 primer set (139 bp)
Lane 2; P55/53 primer set (211 bp)
Lane 3; P55/63 primer set (447 bp)
Lane 4; P75/83 primer set (618 bp)
Lane 5; P55/73 primer set1082 bp)
Lane 6; P65/83 primer set (1296 bp)
Lane 7; P55/83 primer set (1561 bp)



accupower
Figure 3. Comparison of PCR amplification specificity between AccuPower® Hotstart Pfu PCR PreMix from Bioneer and other suppliers' pfu DNA polymerase.
Three different primer-template systems were amplified under the same conditions. The cycling conditions were 95°C for 5min, 35 cycles of 95°C for 30 sec, 62°C for 30 sec, and 72°C for 1 min 30 sec, and 72°C for 5 min for final extension.This data shows that AccuPower® Hotstart Pfu PCR PreMix has higher amplificition efficency and specificity than other suppliers' pfu DNA polymerase.
Lane M; 100 bp DNA Ladder (Cat. No. D-1030,Bioneer) 
Lane 1; 100 ng DNA, ApoE primer set (268 bp)
Lane 2; 10 ng DNA, ApoE primer set (268 bp)
Lane 3; 100 ng DNA, PrP primer set (500 bp)
Lane 4; 10 ng DNA, PrP primer set (500 bp)
Lane 5; 100 ng DNA, PrP primer set (705 bp)
Lane 6; 10 ng DNA, PrP primer set (705 bp)


accupower
Figure 4. AccuPower® Hotstart Pfu PCR PreMix has high amplification efficiency.
Bioneer reaction mixture was followed by 95°C for 5min, 35 cycles of 95°C for 20 sec, 65°C for 20 sec, and 68°C for 15 min , and 68°C for 5 min for final extension.
Lane M; 1 kb DNA Ladder (Cat. No. D-1040,Bioneer) 
Lane 1; 2 kb fragment
Lane 2; 2.5 kb fragment
Lane 3; 3 kb fragment
Lane 4; 4.5 kb fragment
Lane 5; 5 kb fragment


Cat. No. Product Description Price
K-2301 AccuPower® HotStart Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps /96 tubes, 20 μl reaction ₩97,000
K-2302 AccuPower® HotStart Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps /96 tubes, 50 μl reaction ₩146,000
K-2303 AccuPower® HotStart Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes,20 μl reaction ₩364,000
K-2304 AccuPower® HotStart Pfu PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps /480 tubes, 50 μl reaction ₩546,000




















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