특장점
-
Extraction Kit와 MagListo™ Magnetic Separation Rack 외에 고가의 추가 장비 불필요
-
Plasma, Serum, Urine, Saliva 등 여러 샘플에서 cell free DNA 추출
-
Mini, Midi, Maxi scale을 하나의 Kit로 사용 가능
응용 분야
• PCR
• Real-time PCR
• Digital PCR
• Next-generation sequencing (NGS)
▶ 제품 규격/사양
| Sample type |
Mini |
Midi |
Maxi |
| Plasma / Serum / Urine / Saliva |
~ 1 ml |
~ 3 ml |
~ 5 ml |
| Elution volume |
30 μl |
50 μl |
70 μl |
| Typical DNA yield |
~5 ng |
~ 15 ng |
~ 25 ng |
*시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.
▶ 구성품
| Reagent |
*K-3619 |
| CL buffer |
110 ml x 1 ea |
| CB buffer |
110 ml x 1 ea |
| CW1 buffer |
110 ml x 1 ea |
| TA buffer |
120 ml x 1 ea |
| EA buffer |
25 ml x 1 ea |
| Magnetic Nanobead |
11 ml x 1 ea |
| Proteinase K |
25 mg x 2 ea |
*mini - 50 rxn, midi - 16 rxn, maxi - 10 rxn
▶ 실험 자료
| a) Plasma |
b) Urine |
c) Saliva |
 |
 |
 |
Figure 1. Size of cfDNA from EDTA human plasma, human urine and human saliva mixed Bioneer ladder purified with MagListo™ cfDNA Extraction Kit.
M: Bioneer 25/100bp mixed ladder (Cat. No. 20-D-1020)
a) 1-3: cfDNA of plasma mixed Bioneer ladder purified with Bioneer MagListo™ Kit
b) 1-3: cfDNA of urine mixed Bioneer ladder purified with Bioneer MagListo™ Kit
c) 1-3: 1-3: cfDNA of saliva mixed Bioneer ladder purified with Bioneer MagListo™ Kit
| a) Plasma |
b) Serum |
 |
 |
Figure 2. cfDNA from EDTA human plasma, human serum purified with MagListo™ cfDNA Extraction Kit
M: Bioneer 25/100bp mixed ladder (Cat. No. 20-D-1020)
a) cfDNA of plasma mixed Bioneer ladder purified with Bioneer MagListo™ Kit
b) cfDNA of serum purified with Bioneer MagListo™ Kit
a) Serum
Figure3. Comparison of cell free DNA from normal human serum (Millipore, 2 ml) purified with MagListo™ cfDNA Extraction kit and competitor’s kit (magnetic bead type).
M: Bioneer 25 /100 bp mixed ladder
1-3: cell free DNA of serum purified with Bioneer MagListo™
4-6: cell free DNA of serum purified with competitor T kit
