AccuPrep® Genomic DNA Extraction Kit (100 reactions)

AccuPrep® Genomic DNA Extraction Kit는 blood, lymphocyte, buffy coat, tissue, cultured cell로부터 genomic DNA를 빠르게 추출할 수 있는 제품입니다. 본 제품은 높은 DNA binding capacity를 갖는 silica filter가 사용된 spin column 방식의 제품으로, 다양한 시료에 최적화된 lysis buffer와 binding buffer를 비롯하여 washing buffer 및 elution buffer로 구성되어 있습니다. AccuPrep® Genomic DNA Extraction Kit에 의해 추출된 genomic DNA는 gene cloning, PCR, Quantitative real time PCR, southern blotting 등 다양한 실험에 사용할 수 있습니다.

₩124,000
In stock
SKU
K-3032

Features and Benefits

  • 혈액, 동물 조직, 배양 세포 등 다양한 샘플 시료로부터 고순도, 고수율의 genomic DNA 추출 가능
  • 높은 binding capacity를 갖는 silica based DNA binding column 사용
  • 동물 조직 세포를 위한 최적화된 Tissue lysis buffer 제공
  • 유기용매를 사용하지 않아 매우 안전하며, ethanol precipitation 과정이 없어 사용이 편리함


Components K-3032
(100 reaction)
Tissue Lysis buffer (TL) 25 ml X 1 ea
Binding buffer (GC) 25 ml X 1 ea
Wahsing buffer 1 (W1) 40 ml X 1 ea
Washing buffer 2 (W2) 20 ml X 2 ea
Elution buffer (EL) 30 ml X 1 ea
Proteinase K, lyophilized 25 ml X 1 ea
DNA binding column tube 100 ea X 1 pack
2.0ml tubes for filtration 100 ea X 1 pack
1.5ml tubes for elution 100 ea X 1 pack
Manual 1 ea




Specifications

Sample Amount Yield
Whole blood 200 µl 3 - 6 µg
Buffy coat 200 µl 15 - 20 µg
Cultured cells 104 ~ 108 cells 15 - 20 µg
Mammalian tissue 25 ~ 50 mg 10 - 15 µg

 


Applications

Gene cloning
PCR
Real-time PCR
Southern blotting
SNP genotyping


Procedure

accupower


Experimental Data

accupower

Figure 1. Gel electrophoresis of whole blood genomic DNA
Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (Cat. No. D-1070, Bioneer)
M2; Molecular weight marker, 1 kb ladder (Cat. No. D-1040, Bioneer), Lane 1; whole blood genomic DNA of two individual mice
Lane 2; whole blood genomic DNA of two individual rats, Lane 3; whole blood genomic DNA of two persons.
* 100 ng of intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)

accupower
Figure 2. PCR with p53 primer set after genomic DNA purification
Lane M; Molecular marker, 100 bp ladder (D-1030, Bioneer)
Lane 1~8; PCR product with p53 primer set





Manual

• Manual_AccuPrep Genomic DNA Extraction Kit


Brochure

AccuPrep® nucleic acid extraction kit series


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate


















Cat. No. Product Description Price
K-3032 AccuPrep® Genomic DNA Extraction Kit,100 reactions ₩124,000
KB-0111 Proteinase K Powder, 25 mg X 2 ₩70,000






















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