AccuPrep® Stool DNA Extraction Kit (100 reactions)

AccuPrep® Stool DNA Extraction Kit는 사람을 비롯한 다양한 동물의 fresh 혹은 frozen stool 로부터 고순도의 genomic DNA를 추출할 수 있는 제품입니다. 본 제품은 stool내에 포함되어 있는 다양한 PCR 저해물질(heparin, bilirubin 등)들을 효과적으로 제거할 수 있는 Stool Lysis Buffer가 포함되어 있어 매우 편리하게 genomic DNA를 추출할 수 있습니다. 또한, 본 제품은 silica based DNA binding column이 사용된 spin column 방식의 제품으로 binding buffer와 washing buffer 및 elution buffer가 포함되어 있습니다.

₩133,000
카탈로그 번호
K-3036

Features and Benefits

  • 사람 및 다양한 동물들의 stool로부터 고순도의 genomic DNA 추출 가능
  • 다양한 stool sample에 최적화된 Stool Lysis buffer를 사용하여 다양한 PCR 저해물질(heparin, bilirubin 등)이 완벽히 제거된 genomic DNA 추출 가능
  • 높은 binding capacity를 갖는 silica based DNA binding column 사용
  • 유기용매를 사용하지 않아 매우 안전하며, Ethanol precipitation 과정이 없어 사용이 편리함


Components K-3036
(100 reaction)
Stool Lysis Buffer (SL) 50 ml X 1 ea
Binding Buffer (ST) 50 ml X 1 ea
Washing Buffer 1 (W1) 40 ml X 1 ea
Washing Buffer 2 (W2) 20 ml X 1 ea
Elution Buffer (E 25 ml X 1 ea
Proteinase K, lyophilized 25 mg X 2 ea
DNA binding column tube 100 ea X 1 pack
2.0 ml tubes for filtration 100 ea X 1 pack
1.5 ml tube for elution 100 ea X 1 pack
Manual 1 ea




Applications

PCR
Real-time PCR
Southern blotting
SNP genotyping
Pathogen characterization


Procedure

Stool_DNA_Extraction_Ki


Experimental Data

Sample Amount Yield
Stool 100 mg 2 ~ 5 µg

 Table 1. Typical Yield.

Stool_DNA_Extraction_Ki

Figure 1. Gel electrophoresis of stool genomic DNA extracted by AccuPrep® Stool DNA Extraction Kit
Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (D-1070, Bioneer)
Lane M2; Molecular weight marker, 1 kb ladder (D-1040, Bioneer)
Lane 1~3; DNA purified with Bioneer's AccuPrep® Stool DNA Extraction Kit.
100 ng of intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)  

Stool_DNA_Extraction_Ki
Figure 2. PCR results of H. pylori DNA extracted with AccuPrep Stool DNA Extraction Kit
Lane M; Molecular weight marker, 100 bp ladder (D-1030, Bioneer)
Lane 1~4; PCR results for H. pylori detection



Manual

• Manual_AccuPrep Stool DNA Extraction Kit


Brochure

AccuPrep® nucleic acid extraction kit series


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate


















Cat. No. Product Description Price
K-3036 AccuPrep® Stool DNA Extraction Kit,100 reactions ₩133,000
KB-0111 Proteinase K Powder, 25 mg X 2 ₩70,000






















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