MagListo™ 5M Genomic DNA Extraction Kit (100 reactions)


MagListo™ 5M Genomic DNA Extraction Kit 는 magnetic nano bead와 MagListo™ Magnetic Separation Rack을 이용하여 whole blood, cultured cell, animal tissue, bacteria 등 여러 샘플에서 genomic DNA를 빠르게 추출할 수 있는 제품입니다. 자성분리방식으로 세포 분쇄물 중 genomic DNA만을 분리시키고 농축 및 정제하는 과정을 거치기 때문에 원심분리방법에 비해 빠르고 간편하게 DNA를 분리할 수 있습니다. 본 제품은 한 kit를 사용하여 mini, midi, maxi scale로 prep할 수 있어 별도의 kit를 구매할 필요가 없으며, midi나 maxi prep을 위한 vacuum system이나 air pressure system을 구비할 필요가 없어 실험비용을 절감할 수 있습니다.

₩199,000
카탈로그 번호
K-3603

Features and Benefits

  • Extraction Kit와 MagListo™ Magnetic Separation Rack 외에 고가의 추가 장비 불필요.
  • Blood, Cultured cell, Animal Tissue, Bacteria (Gram (-), (+)) 등 여러 샘플에서 genomic DNA추출 가능.
  • Mini, Midi, Maxi scale의 kit를 따로 구매할 필요 없이 한 kit로 해결.
  • 104 cell 이하의 소량 샘플에서 genomic DNA추출 가능.
  • Magnetic Nano Bead를 이용하여 Mini-5분, Midi-10분, Maxi-15분 만에 genomic DNA 추출 가능.

Reagent *K-3603
Buffer ① (Lysis) 25 ml x 1 ea
Buffer ② (Binding) 25 ml x 1 ea
Buffer ③ (1st Washing) 120 ml x 1 ea
Buffer ④ (2nd Washing) 80 ml x 1 ea
Buffer ⑤ (3rd Washing) 120 ml x 1 ea
Buffer ⑥ (Elution) 25 ml x 1 ea
Magnetic Nano Bead - DNA 1.8 ml x 6 ea
Proteinase K 25 mg x 2 ea
RNase A 24 mg x 2 ea
 *mini-100 rxn, midi-10 rxn, maxi-5 rxn

Specifications

Sample Type Mini
(typical yield (μg))
Midi
(typical yield (μg))
Maxi
(typical yield (μg))
Whole blood 200 μl (< 10 μg) 2 ml (< 80 μg) 4 ml (< 150 μg)
Cultured cell Up to 1 x 106 (< 12 μg) Up to 5 x 106 (< 60 μg) Up to 1 x 107 (< 120 μg)
Animal tissue Up to 25 mg (< 10 μg) Up to 100 mg (< 40 μg) Up to 250 mg (< 120 μg)
Bacteria (Gram (-), (+)) Up to 1 x 109 (< 15 μg) Up to 5 x 109 (< 80 μg) Up to 1 x 1010 (< 150 μg)
Expected purity A260/280 > 1.8

Applications

Gene cloning
PCR
Real-time PCR
Southern blotting
SNP genotyping


Experimental Data

Figure 1. Comparison of genomic DNA from human whole blood (200 μl) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-4; Blood genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 6.45 μg, mean purity; 1.85
5-7; Blood genomic DNA purified with competitor Q kit, mean yield; 5.53 μg, mean purity; 1.85


Figure 2. Genomic DNA from 100 μl and 1 ml of human whole blood purified with MagListo™ 5M Genomic DNA Extraction Kit.

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from 100 μl of blood, mean yield; 3 μg, mean purity; 1.80
3-6; (Midi)Genomic DNA from 1 ml of blood, mean yield; 30.85 μg, mean purity; 1.82


Figure 3. Genomic DNA from 200 μl and 4 ml of human whole blood purified with MagListo™ 5M Genomic DNA Extraction Kit.

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from 200 μl of blood, mean yield; 6.65 μg, mean purity; 1.84
3; (Maxi)Genomic DNA from 4 ml of blood, yield; 127.5 μg, purity; 1.88


Figure 4. Comparison of genomic DNA from HeLa cell (1 x 106) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-4; Cell genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 24.1 μg, mean purity; 1.92
5-6; Cell genomic DNA purified with competitor Q kit, mean yield; 23.3 μg, mean purity; 2.00


Figure 5. Genomic DNA from 1x106 and 5x106 of HeLa cell purified with MagListo™ 5M Genomic DNA Extraction Kit.

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from HeLa cell (1x106), mean yield; 13.6 μg, mean purity; 1.83
3-4; (Midi)Genomic DNA from HeLa cell (5x106), mean yield; 75.3 μg, mean purity; 1.83


Figure 6. Comparison of genomic DNA from bovine skeletal muscle tissue (15 mg) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-4; Tissue genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 5.48 μg, mean purity; 1.86
5-6; Tissue genomic DNA purified with competitor Q kit, mean yield; 5.60 μg, mean purity; 1.85


Figure 7. Genomic DNA from 20 mg, 100 mg and 250 mg of porcine skeletal muscle tissue purified with MagListo™ 5M Genomic DNA Extraction Kit.

M; Bioneer 1 kb ladder
1-3; (Mini)Genomic DNA form 20 mg of tissue, mean yield; 5.48 μg, mean purity; 1.81
4-5; (Midi)Genomic DNA form 100 mg of tissue, mean yield; 23.9 μg, mean purity; 1.81
6; (Maxi)Genomic DNA form 250 mg of tissue, yield; 63.4 μg, purity; 1.81


Figure 8. Comparison of genomic DNA from E. coli purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from E. coli (1 x 109) purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 14.7 μg, mean purity; 1.83
3-4; (Mini)Genomic DNA from E. coli (1 x 109) purified with competitor Q kit, mean yield; 15.5 μg, mean purity; 2.05
5-6; (Midi)Genomic DNA from E. coli (5 x 109), mean yield; 83.9 μg, mean purity; 1.80
7; (Maxi)Genomic DNA from E. coli (1 x 1010), yield; 141.6 μg, purity; 1.78


Figure 9. Comparison of real-time qPCR data of genomic DNA from HeLa cell (1 x 101 - 1 x 105) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

A. Amplication curve of GAPDH from HeLa cell (1 x 101 - 1 x 105) genomic DNA purified with Bioneer
    MagListo™ 5M Genomic DNA Extraction Kit. The premix used was Accupower® Plus DualStar™ qPCR
    PreMix (K-6600).
B. Comparison of standard curves from qPCR results using genomic DNA purified with Bioneer MagListo
    Genomic DNA Extraction Kit and competitor Q kit.



Figure 10. Comparison of DNA extraction time from blood (200 μl) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

N: Number of sample


Figure 11. Comparison of DNA extraction time from blood purified with MagListo™ 5M Genomic DNA Extraction Kit.

N: Number of sample


Manuals

Manual_MagListo™ 5M Genomic DNA Extraction Kit

MSDS

• MSDS_MagListo™ 5M Genomic DNA Extraction Kit


Brochure

MagListo™ nucleic acid extraction kit series


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate










Cat. No. Product Description Price
K-3603 MagListo™ 5M Genomic DNA Extraction Kit, 100 rxn in mini ₩199,000

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