MagListo™ 5M Genomic DNA Extraction Kit (100 reactions)


MagListo™ 5M Genomic DNA Extraction Kit 는 Magnetic Nanobead가 적용된 제품으로서 Magnetic Separation Rack을 이용하여 혈액, 동물 조직, 배양 세포로부터 genomic DNA를 빠르게 추출할 수 있는 제품입니다. 추출된 genomic DNA는 gene cloning, PCR, Quantitative real time PCR, southern blotting 등 다양한 실험에 사용할 수 있습니다.

Magnetic bead 방식

₩199,000
카탈로그 번호
K-3603

특장점

  • Blood, Cultured cell, Animal Tissue, Bacteria (Gram (-), (+)) 등 다양한 시료로부터 genomic DNA추출 가능

  • 104 cell 이하의 소량 샘플에서도 genomic DNA 추출 가능

  • Mini, Midi, Maxi scale을 하나의 kit로 사용 가능 (Mini-5분, Midi-10분, Maxi-15분 만에 추출)

  • Extraction Kit와 MagListo™ Magnetic Separation Rack 외에 고가의 추가 장비 불필요

응용 분야

• Gene cloning

• PCR

• Real-time PCR

• Southern blotting

• SNP genotyping

제품 규격/사양

Scale Mini
(typical yield (μg))
Midi
(typical yield (μg))
Maxi
(typical yield (μg))
Whole blood 200 μl (< 10 μg) 2 ml (< 80 μg) 4 ml (< 150 μg)
Cultured cell Up to 1 x 106 (< 12 μg) Up to 5 x 106 (< 60 μg) Up to 1 x 107 (< 120 μg)
Animal tissue Up to 25 mg (< 10 μg) Up to 100 mg (< 40 μg) Up to 250 mg (< 120 μg)
Bacteria (Gram (-), (+)) Up to 1 x 109 (< 15 μg) Up to 5 x 109 (< 80 μg) Up to 1 x 1010 (< 150 μg)
Expected purity A260/280 > 1.8

* 시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.

구성품

구성품 K-3603*
Buffer ① (Lysis) 25 ml x 1 ea
Buffer ② (Binding) 25 ml x 1 ea
Buffer ③ (1st Washing) 120 ml x 1 ea
Buffer ④ (2nd Washing) 80 ml x 1 ea
Buffer ⑤ (3rd Washing) 120 ml x 1 ea
Buffer ⑥ (Elution) 25 ml x 1 ea
RNase A powder, lyophilized 24 mg x 2 ea
Proteinase K, lyophilized 25 mg x 2 ea
Magnetic Nanobead 1.8 ml x 6 ea
사용설명서 1 ea

* mini-100 rxn, midi-10 rxn, maxi-5 rxn

실험 자료

M; Bioneer 1 kb ladder
1-4; Blood genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 6.45 μg, mean purity; 1.85
5-7; Blood genomic DNA purified with competitor Q kit, mean yield; 5.53 μg, mean purity; 1.85

Figure 1. Comparison of genomic DNA from human whole blood (200 μl) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from 100 μl of blood, mean yield; 3 μg, mean purity; 1.80
3-6; (Midi)Genomic DNA from 1 ml of blood, mean yield; 30.85 μg, mean purity; 1.82

Figure 2. Genomic DNA from 100 μl and 1 ml of human whole blood purified with MagListo™ 5M Genomic DNA Extraction Kit.

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from 200 μl of blood, mean yield; 6.65 μg, mean purity; 1.84
3; (Maxi)Genomic DNA from 4 ml of blood, yield; 127.5 μg, purity; 1.88

Figure 3. Genomic DNA from 200 μl and 4 ml of human whole blood purified with MagListo™ 5M Genomic DNA Extraction Kit.

M; Bioneer 1 kb ladder
1-4; Cell genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 24.1 μg, mean purity; 1.92
5-6; Cell genomic DNA purified with competitor Q kit, mean yield; 23.3 μg, mean purity; 2.00

Figure 4. Comparison of genomic DNA from HeLa cell (1 x 106) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from HeLa cell (1x106), mean yield; 13.6 μg, mean purity; 1.83
3-4; (Midi)Genomic DNA from HeLa cell (5x106), mean yield; 75.3 μg, mean purity; 1.83

Figure 5. Genomic DNA from 1x106 and 5x106 of HeLa cell purified with MagListo™ 5M Genomic DNA Extraction Kit.

M; Bioneer 1 kb ladder
1-4; Tissue genomic DNA purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 5.48 μg, mean purity; 1.86
5-6; Tissue genomic DNA purified with competitor Q kit, mean yield; 5.60 μg, mean purity; 1.85

Figure 6. Comparison of genomic DNA from bovine skeletal muscle tissue (15 mg) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

M; Bioneer 1 kb ladder
1-3; (Mini)Genomic DNA form 20 mg of tissue, mean yield; 5.48 μg, mean purity; 1.81
4-5; (Midi)Genomic DNA form 100 mg of tissue, mean yield; 23.9 μg, mean purity; 1.81
6; (Maxi)Genomic DNA form 250 mg of tissue, yield; 63.4 μg, purity; 1.81

Figure 7. Genomic DNA from 20 mg, 100 mg and 250 mg of porcine skeletal muscle tissue purified with MagListo™ 5M Genomic DNA Extraction Kit.

M; Bioneer 1 kb ladder
1-2; (Mini)Genomic DNA from E. coli (1 x 109) purified with Bioneer MagListo™ 5M Genomic DNA Extraction Kit, mean yield; 14.7 μg, mean purity; 1.83
3-4; (Mini)Genomic DNA from E. coli (1 x 109) purified with competitor Q kit, mean yield; 15.5 μg, mean purity; 2.05
5-6; (Midi)Genomic DNA from E. coli (5 x 109), mean yield; 83.9 μg, mean purity; 1.80
7; (Maxi)Genomic DNA from E. coli (1 x 1010), yield; 141.6 μg, purity; 1.78

Figure 8. Comparison of genomic DNA from E. coli purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).


Figure 9. Comparison of real-time qPCR data of genomic DNA from HeLa cell (1 x 101 - 1 x 105) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).

A. Amplication curve of GAPDH from HeLa cell (1 x 101 - 1 x 105) genomic DNA purified with Bioneer
    MagListo™ 5M Genomic DNA Extraction Kit. The premix used was Accupower® Plus DualStar™ qPCR
    PreMix (K-6600).
B. Comparison of standard curves from qPCR results using genomic DNA purified with Bioneer MagListo
    Genomic DNA Extraction Kit and competitor Q kit.




Figure 10. Comparison of DNA extraction time from blood (200 μl) purified with MagListo™ 5M Genomic DNA Extraction Kit and competitor’s kit (single column type).
N: Number of sample



Figure 11. Comparison of DNA extraction time from blood purified with MagListo™ 5M Genomic DNA Extraction Kit.
N: Number of sample

Manuals

Manual_MagListo™ 5M Genomic DNA Extraction Kit


MSDS

• MSDS_MagListo™ 5M Genomic DNA Extraction Kit


Brochure

MagListo™ nucleic acid extraction kit series


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate


카탈로그 번호 제품명 가격
K-3603 MagListo™ 5M Genomic DNA Extraction Kit, 100 rxn in mini ₩199,000

관련 제품

카탈로그 번호 제품명 가격
1750-00 1 ml tube with 8-cap strip, 100 ea / pk ₩35,000
50015 15 ml tube, 500 ea / pk ₩79,200
50050 50 ml tube, 500 ea / pk ₩107,800
HT-15-NG Graduated 1.5 ml Microcentrifuge Tubes, Natural, 500 ea ₩19,200
HT-20-NG Standard 2.0 ml Microcentrifuge Tubes, Natural, 500 ea ₩21,600
TM-1010 MagListo™-2 Magnetic Separation Rack, 2 ml tube x 8 holes ₩255,000
TM-1020 MagListo™-15 Magnetic Separation Rack, 15 ml tube x 6 holes ₩455,000
TM-1030 MagListo™-50 Magnetic Separation Rack, 50 ml tube x 3 holes ₩455,000
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