AccuPower® 2X GreenStar™ qPCR Master Mix

AccuPower® 2X GreenStar™ qPCR  Master Mix는 dsDNA binding dye와 바이오니아의 PyroHotStart 특허기술을 적용하여 반응특이성과 PCR 증폭효율을 높인 Real-Time PCR 제품입니다.

₩131,000
카탈로그 번호
K-6251-CFG

특장점

  • 특이성

    Hotstart Taq DNA polymerase을 사용하여 비특이 증폭산물에 의한 실험 실패를 최소화하고, 미량의 template DNA를 효과적으로 증폭할 수 있습니다.

  • 편리성

    2X master mix type으로 dsDNA binding dye를 따로 첨가하지 않고 solution mix 하는 데 발생될 수 있는 error 를 최소화하여 손쉽게 사용이 가능합니다.

  • 안정성

    Real time PCR 반응물에 안정화제가 들어 있어 장기간 냉동보관이 가능하며 오랫동안 성능이 유지을 유지할 수 있습니다.

  • 재현성

    ISO 9001 품질 시스템 하에서 대량 생산되어 lot 간 편차가 최소화 되었으며, 반복적인 실험에도 재현성 있는 실험 결과를 제공합니다.

응용 및 적용

• Real-time quantification of DNA and cDNA targets

• Gene expression profiling

• Microbial & Viral pathogen detection

실험 자료

accupower figure1

Figure. Highly reproducible Ct values
Amplification of an 90 bp target gene was detected using serially diluted LP(Legionella Pneumoniae) genomic DNA (106~101copies) with AccuPower® 2X Greenstar™ qPCR master mix. As shown in Figure, highly reproducible Ct values were achieved within each Lot. set of triplicates

제품 규격/사양

5' to 3' exonuclease activity

No

3' to 5' exonuclease activity

No

3' – A Overhang

Yes

실험 자료

Equipment Compatibility

Using a Bioneer Exicycler™ 96

- The annealing temperature can be set to 55~65°C, depending on the primer Tm value.

- The annealing time should be set for 5~20 seconds. Longer annealing time results in increased efficiency, and a shorter time decreases non-specific amplification.

accupower figure1

Using a ABI 7500

accupower figure1

Using a Bio-Rad

accupower figure1

Figure 1. Comparison of the specificity of the intercalating dye-based real-time PCR products. Amplification of a 90 bp target gene was detected using serially diluted LP (Legionella Pneumoniae) genomic DNA (10ndilution;105~101copies). As shown in the Figure 1, very small amount of primer dimers was observed in AccuPower® 2X GreenStar™ qPCR Master mix compared with other kits.




accupower figure1

Figure 2. Gene expression analysis AccuTarget ™ Validated Real-Time PCR Primer Library for Human is designed by Bioneer’s bioinformatics tool and targeting for human genome.  cDNA was synthesized using  human PTGS2 target primer of those and human total RNA identically quantified from Hela cell and blood cell with AccuPower® CycleScript RT PreMix (Cat. No. K-2044, Bioneer). Gene analysis was carried out both Hela cell and blood cell by operating real-time PCR reaction (95°C 10 min, 1 cycle and 95°C 10 sec, 58°C 25 sec, 72°C 30 sec, 41 cycles) using the cDNA, AccuPower® GreenStar™ qPCR Master Mix and Exicycler™ 96 Real-Time Thermal Block (Cat. No. A-2060, Bioneer).

Manual

Manual_AccuPower® 2X GreenStar™ qPCR Master Mix


MSDS

MSDS_AccuPower® 2X GreenStar™ qPCR Master Mix


Brochure

AccuPower® 2X GreenStar™ qPCR Master Mix_Flyer

All about Real-Time PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate


카탈로그 번호 제품명 가격
K-6251 AccuPower® 2X Greenstar™ qPCR Master Mix with 80X ROX Dye / 100 Rxn, 50 μL reaction ₩138,000
K-6252 AccuPower® 2X Greenstar™ qPCR Master Mix with 80X ROX Dye / 200 Rxn, 50 μL reaction ₩235,000
K-6253 AccuPower® 2X Greenstar™ qPCR Master Mix / 100 Rxn, 50 μL reaction ₩131,000
K-6254 AccuPower® 2X Greenstar™ qPCR Master Mix / 200 Rxn, 50 μL reaction ₩233,000
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