MagListo™ Protein G Kit (20 reactions)
특장점
- 신속성
Magnetic nanobead의 강력한 자성력으로 실험을 보다 빠르고 손실 없이 진행할 수 있습니다.
- 우수한 결합력
평균 직경 400 nm의 magnetic nanobead는 표면적이 넓고 결합력이 우수하여 높은 binding capacity를 보장합니다.
- Specificity
균일한 구형의 nanobead 사용으로 비특이적 결합을 감소시켰습니다.
응용 분야
• 항체정제
• immunoprecipitation
• 항원-항체 상호작용 연구
• 단백질 복합체 연구
• cell separation
▶ 제품 구성
| 구성품 | 보관 온도 | |
| AccuNanoBead ™ Protein G Magnetic NanoBeads | 1 ml x 1 ea (4%) | 2 - 8°C 보관 |
| Binding & Washing Buffer | 20 ml x 2 ea | |
| Elution Buffer | 1 ml x 2 ea | |
| Neutralization Buffer | 1 ml | |
| Manual | 1 ea | |
▶ 제품 사양
| Feature | Specification |
| Matrix | Silica-coated Fe3O4 |
| Concentration | 40 mg/ml (4% suspension) |
| Max. Binding Capacity | > 30 μg Human IgG/mg beads sample |
| Storage and Stability | Beads can be stored at 2 - 8°C until the expiration date. Do not freeze and vortex vigorously. |
▶ Binding affinity table
▶ 실험 결과
Figure 1. Comparison of protein binding efficiency of AccuNanoBead ™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit with a competitor.
A) Elution fraction contains Human IgG purified with Protein G magnetic bead from Bioneer(MagListo™), a competitor L or a competitor Q. Upper and lower bands are heavy (HC) and light chain (LC) of purified Human IgG in SDS-PAGE gel, respectively.
B) Comparison of binding capacity of Bioneer’s MagListo™ and competitor’s. Amount of bound IgG is quantified by UV-spectrophotometer. All reactions are carried out for 1 hour at room temperature. Human IgG is purchased from Sigma (Cat. no. I4506). M: protein marker (Bioneer, Cat. No. D-2020), Bioneer: MagListo™ Protein G Kit.
Figure 2. Purification of Human IgG with AccuNanoBead™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit.
IgG was purified from human whole blood. Arrows point to heavy and light chains of IgG, respectively.
- M: protein marker
- W: human whole blood
- U: unbound fraction
- E1 & 2: elution fraction 1 & 2.
◈ Manual
• Manual_MagListo™ Protein G Kit
◈ MSDS
• MSDS_MagListo™ Protein G Kit_Magnetic Nanobeads
• MSDS_MagListo™ Protein G Kit_Binding&Washing buffer
• MSDS_MagListo™ Protein G Kit_Elution buffer
• MSDS_MagListo™ Protein G Kit_ Neutralization buffer
◈ Brochure
◈ Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
| 카탈로그 번호 | 제품명 | 가격 |
| K-7710 | MagListo™ Protein G Kit, 1 x 1 mL | ₩210,000 |
▶ 관련 제품
| 카탈로그 번호 | 관련 제품 |
| TA-1021-1 | AccuNanoBead ™ Protein G Magnetic Nanobeads, size 400 nm, 40 mg/1 ml |
| TA-1021-2 | AccuNanoBead ™ Protein G Magnetic Nanobeads, size 400 nm, 200 mg/10 ml |
| TA-1021-3 | AccuNanoBead ™ Protein G Magnetic Nanobeads, size 400 nm, 500 mg/25 ml |
| TM-1010 | MagListo ™-2 Magnetic Separation Rack, 2 ml tube x 8 holes |
바이오니아의 재조합단백질 합성 및 정제에 관련된 제품군은 아래와 같이 분류되며, 용도에 따라서 선택이 가능합니다.
Exiprogen™ 데모 신청은 sales@bioneer.co.kr 로 문의하여 주시기 바랍니다.
| 과정 | 제품 종류 | 사용 장비 | 서비스 |
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DNA Template 준비 |
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단백질 합성 / 정제 / 투석 |
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