MagListo™ Protein G Kit (20 reactions)
MagListo™ Protein G Kit는 자성분리방식으로 항체를 빠르고 간편하게 정제할 수 있는 제품으로, Protein G Magnetic Nanobead와 항체 정제에 필요한 buffer로 구성되어 있습니다. Magnetic Nanobead는 고순도(> 95%) protein G가 코팅된 silica bead를 사용하고 있으며 silica bead안에는 magnetic particles이 포함되어 있습니다. 이러한 bead는 특이적으로 항체를 인지하여 항체정제, immunoprecipitation, 항원-항체 상호작용 연구, 단백질 복합체 연구, cell separation 등에 응용할 수 있습니다.
Features and Benefits
- Fast Binding: 강력한 자성력은 bead의 빠른 회수율로 실험을 보다 빠르고 손실 없이 진행할 수 있습니다.
- Large Surface Area: 평균직경 400 nm의 magnetic nanobead는 표면적이 넓고 결합력이 우수하여 높은 binding capacity를 보장합니다.
- Specificity:균일한 구형의 nanobead로 비특이적 결합을 감소시켰습니다.
Kit Components
| Kit components | Cat. No. K-7710 |
| AccuNanoBead™ Protein G Magnetic NanoBeads | 1 ml x 1 ea (4%) |
| Binding & Washing Buffer | 20 ml x 2 ea |
| Elution Buffer | 1 ml |
| Neutralization Buffer | 1 ml |
| Manual | 1 ea |
Specifications
| Feature | Specification |
| Matrix | Silica-coated Fe3O4 |
| Concentration | 40 mg/ml (4% suspension) |
| Max. Binding Capacity | > 30 μg Human IgG/mg beads sample |
| Storage and Stability | Beads can be stored at 2~8°C until the expiration date. Do not freeze and vortex vigorously. |
Application
Antibody purification, Antigen purification, Immunoprecipitation, Protein-protein interaction, and Cell separation etc.
Experimental Data
Figure 1. Comparison of protein binding efficiency of AccuNanoBead™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit with a competitor.
A) Elution fraction contains Human IgG purified with Protein G magnetic bead from Bioneer(MagListo™), a competitor L or a competitor Q. Upper and lower bands are heavy (HC) and light chain (LC) of purified Human IgG in SDS-PAGE gel, respectively.
B) Comparison of binding capacity of Bioneer’s MagListo™ and competitor’s. Amount of bound IgG is quantified by UV-spectrophotometer. All reactions are carried out for 1 hour at room temperature. Human IgG is purchased from Sigma (Cat. no. I4506). M: protein marker (Bioneer, Cat. No. D-2020), Bioneer: MagListo™ Protein G Kit.
Figure 2. Purification of Human IgG with AccuNanoBead™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit.
IgG was purified from human whole blood. Arrows point to heavy and light chains of IgG, respectively.
M: protein marker, W: human whole blood, U: unbound fraction, E1 & 2: elution fraction 1 & 2.
Manual
• Manual_MagListo™ Protein G Kit
MSDS
• MSDS_MagListo™ Protein G Kit_Magnetic Nanobeads
• MSDS_MagListo™ Protein G Kit_Binding&Washing buffer
• MSDS_MagListo™ Protein G Kit_Elution buffer
• MSDS_MagListo™ Protein G Kit_ Neutralization buffer
Brochure
Quality Assurance
Bioneer is the holder of Quality Management System Certificates for the following standards.
| Cat. No. | Product Description | Price |
| K-7710 | MagListo™ Protein G Kit, 1 x 1 mL | ₩210,000 |
Related Products
| Cat. No. | Related Kits | Price |
| TA-1021-1 | AccuNanoBead™ Protein G Magnetic Nanobeads, size 400nm, 40mg/1ml | ₩152,000 |
| TA-1021-2 | AccuNanoBead™ Protein G Magnetic Nanobeads, size 400nm, 200mg/10ml | ₩522,000 |
| TA-1021-3 | AccuNanoBead™ Protein G Magnetic Nanobeads, size 400nm, 500mg/25ml | ₩990,000 |
| TM-1010 | MagListo™-2 Magnetic Separation Rack, 2ml tube x8 holes | ₩255,000 |