AccuNanoBead™ C18 Magnetic Nanobeads, size 400 nm (0.5 g/25 ml)
Magnetic Protein Purification
Magnetic Biosimilar Separation
Magnetic Antibody Separation
FE-SEM and TEM Images of Magnetic Nanobeads
Figure 1. FE-SEM image of Magnetic Nanobeads; SEM images show that Magnetic Nanobeads are sphere shape.
EDS Analysis of Magnetic Nanobeads
Figure 2. EDS analysis result of Magnetic Nanobeads; EDS shows that Magnetic Nanobeads are composed of silica and Iron oxide.
Size Distribution of Magnetic Nanobeads
Figure 3. Size distribution diagram of silica magnetic nanobeads. The average size of magnetic beads is approximately 400 nm.
Protein Purification Method by using Magnetic Nanobeads
First, load the protein sample to tube and then put Magnetic Nanobeads to combine protein sample together with Magnetic Nanobeads. Fix Magnetic Nanobeads with external Magnetic field to wash the solution and then elute protein from Magnetic Nanobeads. Using Magnetic Nanobeads to use in purification is much faster to separate protein compare to an existing method.
Figure 4. Recombinant Protein and Antibody protein purification protocol by Magnetic Nanobeads.
Comparison of Protein Purification Beads
Figure 5. (a) SEM image of 400 nm Bioneer magnetic nanobeads, (b) SEM image of A company magnetic beads, (c) SEM image of B company magnetic beads, (d) SEM image of C company magnetic beads, (e) SEM image of D company magnetic beads Figure 5 shows scanning electron microscope images of Bioneer magnetic nanobeads and different company magnetic beads(b~e). Scanning electron microscope image of synthesized magnetic nanobeads shows that magnetic nanobeads of Bioneer is 400 nm size and spherical shape. And different company magnetic beads are micro meter size and various shape.
Comparison of Protein Purification Yield
The protein purification yields of Bioneer Ni-NTA magnetic nanobeads and other company magnetic beads are listed in Table 1. These results indicate that the purification yield of Bioneer Magnetic beads is higher than that of four different company magnetic beads. In addition, purity of protein purification was more than 90%. His-Tagged DNA Polymerase protein of the 97 kDa expressed in E. coli was used in experiment. Purification yield is amount of purified target protein from loading protein. Bioneer magnetic nanobeads represents the highest purification yield compared with that of four different company magnetic beads because the surface area of magnetic beads is increased due to the size effect.
Table 1. Comparison of protein purification yield of bioneer beads and other company beads.
Figure 6. Comparison of elution protein of bioneer beads and other company beads.
Figure 7. SDS-PAGE image of purified 94 kDa DNA Polymerase with Bioneer magnetic Nanobeads.
Bioneer is the holder of Quality Management System Certificates for the following standards.