T4 DNA Ligase

유전자 재조합 실험에 주로 사용되는 본 제품은 DNA 사이의 결합에 사용됩니다. T4 DNA ligase는 duplex DNA 또는 RNA에 있는 5'-인산기 말단과 3'-수산기 말단 부위 사이에 phosphodiester bond 형성을 촉진하여 duplex DNA, RNA, 또는 DNA/RNA hybrids 에 있는 단일가닥 틈 사이를 repair 할 뿐만 아니라 blunt-end 그리고 cohesive-end 끝 말단 부위로부터 DNA를 연결시킵니다.

₩53,000
In stock
SKU
E-3061-CFG

Features and Benefits

High Speed:
DNA ligations in 5 minutes for cohesive end and in 10 minutes for blunt end DNA at 25°C

Flexibility:
Suitable for all common DNA ligations

Reproducibility:
재현성 있는 결과를 위해 바이오니아의 전 제품은 엄격한 ISO 품질 시스템하에서 생산 됩니다.







Enzyme Properties

Heat Inactivation: 70°C for 10 minutes


Application

Blunt or cohesive-end ligation
Repair of nicks in double-stranded nucleic acids


Reagents Supplied

10 x Reaction Buffer: 500 mM Tris-HCI (pH 7.8), 100 mM MgCl2, 50 mM DTT, 10 mM ATP, 25 μg/ml BSA


Concentration

200U/μl


Storage Conditions

50% glycerol containing 10 mM Tris-HCI (pH 7.5), 50 mM KCI, 1 mM EDTA, 10 mM 2-Mercaptoethanol


Store Temperature

-20°C


Unit Definition

0.01 Weiss unit of enzyme is defined as the amount of enzyme required to give 90% ligation of Hind III fragments of lambda DNA in 30 minutes, at 16°C in 20 μl of the assay mixture.



enzyme ligase figure1

Lane 1: DNA fragment (digested with EcoR V)
Lane 2, 3, 4, 5: T4 DNA Ligase 1 U, 16°C, 10, 20, 30 and 60 minutes
Lane 6, 7, 8, 9: T4 DNA Ligase 1 U, 25°C, 10, 20, 30 and 60 minutes
Lane 10, 11, 12,13: T4 DNA Ligase 1 U, 37°C, 10, 20, 30 and 60 minutes
Lane 14: Lambda DNA (digested with Hind III)
Lane 15, 16, 17: T4 DNA Ligase 1 U, 16°C, 10, 20 and 30 minutes
Lane 18, 19, 20: T4 DNA Ligase 1 U, 25°C, 10, 20 and 30 minutes
Lane 21, 22, 23: T4 DNA Ligase 1 U, 37°C, 10, 20 and 30 minutes

Manual

• Manual_T4 DNA Ligase


MSDS

• MSDS_T4 10X Reaction buffer

• MSDS_T4 DNA Ligase

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

• EN ISO 13485 - certificate


Cat. No. Product Description Price Add to Cart
E-3061 T4 DNA Ligase, 20,000 U ₩53,000 add cart
E-3062 T4 DNA Ligase, 100,000 U(5 x 20,000 U) ₩211,000 add cart


















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