MagListo™ 5M Forensic Sample DNA Extraction Kit (100 reactions)

MagListo™ Forensic Sample DNA Extraction Kit은 Magnetic Nanobead가 적용된 제품으로서 MagListo™ Magnetic Separation Rack을 이용하여 다양한 종류의 법의학 시료로부터 total DNA를 간편하게 추출할 수 있는 제품입니다. 추출된 total DNA는 PCR, Quantitative real time PCR, SNP genotyping, STR analysis 등 다양한 실험에 사용할 수 있습니다.

Magnetic bead 방식

₩300,000
카탈로그 번호
K-3615

특장점

  • Magnetic Nanobead를 이용하여 다양한 법의학 시료(전혈, 타액, 소변, 지문, 머리카락, 손톱, 뼈 등) 로부터 total DNA 추출 가능

  • Extraction Kit와 MagListo™ Separation Rack 외에 고가의 추가 장비 불필요

응용 분야

• PCR

• Real-time PCR

• SNP genotyping

• STR analysis

제품 규격/사양

Feature Specification
Sample type Various kind of forensic sample
Technology Magnetic nano bead
Hands-on time < 10 min
Expected purity A260/280 > 1.8

* 시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.

구성품

구성품 K-3615
Buffer ① (Lysis) 40 ml x 1 ea
Buffer ② (Binding) 30 ml x 1 ea
Buffer ③ (1st Washing) 80 ml x 1 ea
Buffer ④ (2nd Washing) 80 ml x 1 ea
Buffer ⑤ (3rd Washing) 80 ml x 1 ea
Buffer ⑤ (Elution) 15 ml x 1 ea
Poly (A), lyophilized 1 mg x 1 ea
Proteinase K, lyophilized 25 mg x 2 ea
Magnetic Nano Bead - DNA 1.8 ml x 6 ea
사용설명서 1 ea

실험 자료

(A) Urine



Product Sample volume
10 ml 5 ml 1 ml NTC
MagListo 32.79 33.53 35.24 product
32.33 33.08 35.21
Q 32.48 33.36 38.93
32.76 33.08 40.16



(B) Saliva & Blood



Saliva Kit Blood Kit
MagListo Q MagListo
1 µl 33.98 33.40 1 µl 31.42
33.66 33.51 31.32
10 µl 30.55 30.93 10 µl 28.32
30.44 31.28 28.14
100 µl 26.91 30.93 100 µl 28.32
27.66 27.64 25.51
NTC U.D.
U.D.



(C) Saliva (10 ul) & Blood (10 ul) stains from clothes (7 mm diameter)



Sample Kit
MagListo Q
Saliva 30.30 32.05
30.60 32.14
Blood 29.15 29.69
29.14 29.89
NTC U.D.
U.D.



(D) Saliva (10 ul) and fingerprint on paper (7 mm diameter)



Sample Kit
MagListo Q
Saliva 28.07 28.1
28.43 28.59
Fingerprint 36.33 36.45
36.29 36.45
NTC U.D.
U.D.



(E) Saliva on cotton swab and chewing gum



Kit Ct
Swab Gum
MagListo 26.41 32.10
28.29 32.25
Q 26.87 31.21
26.45 31.10
NTC U.D.
U.D.



(F) Hair 1 strand (25 cm)



Sample Kit
MagListo Q
w/ root 29.99 30.7
30.04 30.4
w/o root 33.39 34.66
33.08 34.22
NTC U.D.
U.D.



(G) Nail clipping (15 mg)



Kit Ct
MagListo 28.00
27.98
Q 30.04
29.46
NTC U.D.
U.D.

Figure 1. Comparison of real-time PCR data of DNA extracted using MagListo™ and competitor’s product (single-column type).



Figure 2. Comparison of total DNA from skeletal muscle tissue purified with MagListo™ and competitor’s kit (single column type).

M; 1 kb Ladder
1 mg
MagListo™; yield: 1.35 ug purity(A260/280): 1.95
Competitor; yield: 0.57 ug purity(A260/280): 1.86
5 mg
MagListo™; yield: 5.22 ug purity(A260/280): 1.90
Competitor; yield: 2.81 ug purity(A260/280): 1.88


Figure 3. Comparison of DNA extraction time from forensic sample purified with MagListo™ and competitor’s kit (single column type).

N: Number of Samples

Manual

Manual_MagListo™ 5M Forensic sample DNA Extraction Kit


MSDS

• MSDS_MagListo™ 5M Forensic Sample DNA Extraction Kit


Brochure

MagListo™ nucleic acid extraction kit series


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate


카탈로그 번호 제품명 가격
K-3615 MagListo™ 5M Forensic Sample DNA Extraction Kit, 100 reaction ₩300,000
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