Pfu DNA Polymerase

Pfu DNA Polymerase는 Pyrococus furiosus (bacteria) 에서 유래되었습니다.
3' → 5' exonuclease (proofreading) activity 를 지니고 있어 fidelity가 높은 제품입니다. 또한 뛰어난 특이성을 가지고 있어 nonspecific product의 발생이 적으며, 교정(Proofreading) 기능이 있어 DNA 증폭 시 발생하는 error rate 를 감소시켜주는 제품입니다.

₩90,000
카탈로그 번호
E-2015-CFG

특장점

  • High Fidelity PCR

    3' → 5' exonuclease (proofreading) 활성을 갖습니다.

  • 높은 특이성

    Taq DNA polymerase보다 높은 특이성을 가지고 있어 nonspecific product의 발생이 적습니다.

  • Terminal Transferase Activity

    Terminal transeferase 활성이 없어 blunt-ended PCR product를 보장합니다.

  • 재현성

    ISO 9001 품질시스템 하에서 생산되어 각 batch에 대한 균일한 품질의 제품이 공급되기 때문에 재현성 있는 결과를 얻을 수 있습니다.

응용 및 적용

  • Gene synthesis
  • PCR or primer extension requested high fidelity
  • Blunt-end PCR cloning or mutagenesis requested high fidelity

실험 자료

Amplication of limiting amounts of template DNA


Figure.  Lambda DNA was amplified using 1 units of enzyme in 20 µl reaction volume.

제품 규격/사양

5' to 3' exonuclease activity

No

3' to 5' exonuclease activity

Yes

3' – A overhang

No

Fragment size

< 20 kb

제품 구성

10X reaction buffer: MgSO4, Tris-HCl, KCl, (NH4)2SO4, Acetylated BSA pH 9.0

10 mM dNTPs mix: 2.5 mM of each dNTP

농도

250 Units (2.5 U/μl)

저장 조건

50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, Stablizers, 50 % Glycerol pH 8.2

보관 온도

-20°C

단위(Unit) 정의

One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.

실험 자료

Amplication of limiting amounts of template DNA


Figure 1.  Human DNA was amplified using 1 units of enzyme in 20 µl reaction volume.


Figure 2.  Amplification of fragments ranging from 2 kb to 5 kb from template Lambda DNA 100 pg with 1 units of Pfu DNA Polymerase.

director: yang, sung-jun tel: 649

Manual

Manual_Pfu DNA Polymerase


MSDS

MSDS_10 mM dNTPs

MSDS_10X Reaction buffer for Pfu DNA polymerase

MSDS_Dilution buffer for Pfu DNA polymerase

MSDS_Pfu DNA polymerase


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 – certificate


카탈로그 번호 제품명 가격
D-3001 10 mM dNTP (each 2.5 mM), 1 ml ₩40,000
E-2015 Pfu DNA Polymerase 250 U, 10X reaction buffer, without dNTPs ₩90,000
E-2015-1 Pfu DNA Polymerase 250 U, 10X reaction buffer, with 10 mM dNTPs ₩100,000
E-2016 Pfu DNA Polymerase 1,000 U, 10X reaction buffer, without dNTPs ₩315,000
E-2016-1 Pfu DNA Polymerase 1,000 U, 10X reaction buffer, with 10 mM dNTPs ₩350,000
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