Pfu DNA Polymerase

Pfu DNA Polymerase는 Pyrococus furious라는 bacteria에서 유래되었으며, 3' → 5' exonuclease (proofreading) activity를 지니고 있어 기존의 Taq DNA Polymerase보다 heat stability와 fidelity가 뛰어납니다. 또한 뛰어난 specificity를 가지고 있어 nonspecific product의 발생이 적으며, proofreading 기능이 있어 DNA 증폭 시 발생하는 error rate을 감소시킵니다. Pfu DNA Polymerase를 이용하여 Gene cloning, Polymerase Chain Reaction(PCR), primer extension 등 다양한 실험에 사용하실 수 있습니다.

₩90,000
카탈로그 번호
E-2015-CFG

Features and Benefits

High Fidelity PCR:
3' → 5' exonuclease (proofreading) 활성을 갖습니다.

Thermostability:
95℃에서 한 시간 후 thermostable activity를 94-99% 유지합니다.

Terminal Transferase Activity:
Terminal transeferase 활성이 없어 blunt-ended PCR product를 보장합니다.

Reproducibility:
재현성 있는 결과를 위해 바이오니아의 전 제품은 one batch에서 대량생산되고 엄격한 ISO 품질 검사를 통해 항상 균일한 품질을 보증합니다.






Enzyme Properties

5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: Yes
3' – A overhang: No
Fragment size:  < 20 kb

Applications

Gene synthesis
PCR or primer extension requested high fidelity
Blunt-end PCR cloning or mutagenesis requested high fidelity


Reagents Supplied

10X reaction buffer: MgSO4, Tris-HCl, KCl, (NH4)2SO4, Acetylated BSA pH 8.8
dNTP mix: 2.5 mM of each dNTP (optional)


Concentration

250 U/100 μl


Store Temperature

-20°C


Unit Definition

One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.


Activity Test

Amplication of limiting amounts of template DNA: comparison of Lambda DNA (A), bacterial genomic DNA (B), human genomic DNA (C).

* Lambda DNA was amplified using 2.5 units of enzyme in 50 µL reaction volume.
* Bactierial DNA was amplified using 2.5 units of enzyme in 50 µL reaction volume.

figure1

* Human DNA was amplified using 2.5 units of enzyme in 50 µL reaction volume.


figure2

Amplification of fragments ranging from 500 bp to 5 kb from template Lambda DNA 100 pg with 2.5 units of Pfu DNA Polymerase.


figure3


Notice to Purchaser

FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
This product is not available for sale in the USA.


Manual

• Manual_pfu DNA Polymerase


MSDS

• MSDS_10 mM dNTPs

• MSDS_Pfu 10X Reaction buffer

• MSDS_Pfu Dilution buffer

• MSDS_Pfu DNA polymerase


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

• EN ISO 13485 - certificate


Cat. No. Product Description Price
D-3001 10 mM dNTP (each 2.5 mM), 1 ml ₩40,000
E-2015 Pfu DNA Polymerase 250 U, 10X reaction buffer, without dNTPs ₩90,000
E-2015-1 Pfu DNA Polymerase 250 U, 10X reaction buffer, with 10 mM dNTPs ₩100,000
E-2016 Pfu DNA Polymerase 1,000 U, 10X reaction buffer, without dNTPs ₩315,000
E-2016-1 Pfu DNA Polymerase 1,000 U, 10X reaction buffer, with 10 mM dNTPs ₩350,000












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