ProFi Taq DNA Polymerase

ProFi Taq DNA polymerase 는 Bioneer가 합성 생물학 기술을 이용하여 개발한 recombinant Taq DNA polymerase로써 뛰어난 증폭 효율과 정확성을 동시에 충족시키는 DNA polymerase 입니다. ProFi Taq DNA polymerase는 Taq DNA polymerase를 개선한 효소로써 기존의 Taq DNA polymerase와 비교하여 뛰어난 증폭 성능, 높은 efficiency를 가지는 제품입니다. ProFi Taq DNA polymerase 는 human genomic DNA의 경우 21 kb까지 DNA fragment 증폭이 가능하며 Lambda DNA 의 경우 30 kb까지 DNA fragment 증폭이 가능합니다. ProFi Taq DNA polymerase 는 high effiency 및 long-range PCR에 매우 적합한 제품이며 그 외에도 complex genomic DNA 또는 cDNA templates, low-copy targets등 다양한 PCR 증폭반응에 사용하실 수 있습니다. 

₩100,000
카탈로그 번호
E-2201-CFG

Features and Benefits

Sensitivity:
Lambda DNA 및 human gDNA를 template로 사용하여 sensitivity를 확인한 결과, 타사 제품에 비해 높은 증폭 효율 및 민감도를 가집니다.

Long Range PCR :
Lambda DNA의 경우 30 kb, human gDNA의 경우 21 kb의 PCR 산물을 효과적으로 증폭시킬 수 있습니다.

Reproducibility :

재현성 있는 결과를 위해 바이오니아의 전 제품은 one batch에서 대량생산되고 엄격한 ISO 품질 검사를 통해 항상 균일한 품질을 보증합니다.



Specifications

5' to 3' exonuclease activity : Yes
3' to 5' exonuclease activity : Yes
3' – A overhang : Yes
PCR product size : Up to 30 kb


Applications

- Primer extension
- Long-range amplification from genomic DNA
- High amplification efficiency
- Excellent performance on difficult template
- Amplification of low-copy targets
- High yield and high sensitivity PCR


Experimental Data


Figure 1. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reaction using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target: human insulin receptor gene
Lane M; 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1; 10 ng of human genomic DNA
Lane 2; 1 ng of human genomic DNA
Lane 3; 100 pg of human genomic DNA
Lane 4; 10 pg of human genomic DNA


Figure2. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase. 
cDNA synthesized from 10-fold serial-diluted human total RNA from 10 ng to 10 pg using AccuPower® RocketScript™ Cycle RT PreMix (Bioneer, Cat. No. K-2201) was used as a template for PCR amplification. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 33 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target: human GAPDH gene
Lane M; 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1; 10 ng of human total cDNA
Lane 2; 1 ng of human total cDNA
Lane 3; 100 pg of human total cDNA
Lane 4; 10 pg of human total cDNA


Figure 3 . Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec and 68°C for 4 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Lane M; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 2 kb fragment (human tumor protein p53 gene)
Lane 2; 3 kb fragment (human tumor protein p53 gene)
Lane 3; 4.5 kb fragment (human DNA cross-link repair 1A gene)
Lane 4; 8 kb fragment (human hemoglobin epsilon 1 gene



Figure 4. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec and 68°C for 15 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Human genomic DNA was used as a template for PCR amplification.

Lane M1; Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 11 kb fragment
Lane 2; 13.5 kb fragment
Lane 3; 17.6 kb fragment
Lane 4; 21.4 kb fragment



Figure 5 . Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec, 65°C for40 sec, and 68°C for 20 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Lambda DNA was used as a template for PCR amplification.

Lane M1; Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 15 kb fragment
Lane 2; 20 kb fragment
Lane 3; 25 kb fragment
Lane 4; 30 kb fragment

Cat. No. Product Description Price
E-2201 ProFi Taq DNA Polymerase 250 U, 10 mM dNTPs, 10X reaction buffer with MgCl2 ₩120,000
E-2202 ProFi Taq DNA Polymerase 250 U, 10 mM dNTPs, 10X reaction buffer without MgCl2, 20 mM MgCl2 ₩120,000
E-2203 ProFi Taq DNA Polymerase 250 U, 10X reaction buffer with MgCl2 ₩100,000
E-2204 ProFi Taq DNA Polymerase 250 U, 10X reaction buffer without MgCl2, 20 mM MgCl2 ₩100,000
E-2205 ProFi Taq DNA Polymerase 1000 U, 10 mM dNTPs, 10X reaction buffer with MgCl2 ₩420,000
E-2206 ProFi Taq DNA Polymerase 1000 U, 10 mM dNTPs, 10X reaction buffer without MgCl2, 20 mM MgCl2 ₩420,000
E-2207 ProFi Taq DNA Polymerase 1000 U, 10X reaction buffer with MgCl2 ₩350,000
E-2208 ProFi Taq DNA Polymerase 1000 U, 10X reaction buffer without MgCl2, 20 mM MgCl2 ₩350,000











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