SARS-CoV-2 Mutation Research

COVID-19 virus are constantly evolving to new variants which have different shedding capacity and susceptibility to vaccine, which is mainly caused by the change of spike protein. To prevent the super-spreading of the variants, the S protein should be sequenced more quickly and efficiently. 
AccuPower^® SARS-CoV-2 S gene Amplification Kit provides the more fast and convenient solution for sequencing of S gene. The kit contains every RT-PCR components including primers and enzymes. Just add your RNA sample into 4 tubes of kit, you can amplify whole S gene for direct sanger sequencing.
You can also analyze the S-Protein gene sequence of fewer positive samples such as 100 copies or less using a Nested PCR kit.
Direct Sanger Sequencing is possible after PCR purification without cloning.

Step 2: Viral RNA Extraction

Spin column type	Magnetic Bead type	Automatic type
AccuPrep® Viral RNA Extraction Kit	MagListo™ 5M Viral DNA/RNA Extraction Kit	ExiPrep™96 Lite

카탈로그 번호	제품명	규격
K-3033	AccuPrep® Viral RNA Extraction Kit	100 reactions
K-3624	MagListo™ 5M Viral DNA/RNA Extraction Kit	100 reactions
TM-1011	MagListo™ 2-12h Magnetic Separation Rack	2 ml x 12 holes
A-5250	ExiPrep™96 Lite	-

Step 3: Amplification & Diagnosis

Diagnostics kit	Real-Time PCR
COVID-19 Diagnostics Kit	Exicycler™ 96/384 Real-Time Quantitative Thermal Block

카탈로그 번호	제품명
A-2060-1	Exicycler™ 96 Real-Time Quantitative Thermal Block
A-2061	Exicycler™ 384 Real-Time Quantitative Thermal Block

Step 4: S gene Amplification

AccuPower® SARS-CoV-2 S gene Amplification Kit (K-2520)

Kit Components

workflow

카탈로그 번호	제품명	규격
K-2520	AccuPower® SARS-CoV-2 S gene Amplification Kit	① 20 µl/rxn, 96 tests ② 20 µl/rxn, 24 tests
Related Instruments
A-2041	AllInOneCycler™ PCR System	-
A-7040	ExiSpin™	-

Step 5: PCR Clean-up

카탈로그 번호	제품명	규격
K-3037	AccuPrep® PCR/Gel Purification	200 reactions
K-3038		50 reactions

Step 6: Sanger Sequencing service

Fast and cost-effective Bioneer's Sanger sequencing service

카탈로그 번호	제품명
N-8250	Sequencing Primer set for SARS-CoV-2 S gene Analysis
Sequencing Service

* The primer is free if you use the Sanger sequencing service in Bioneer.