Taq DNA Polymerase

Thermus aquaticus YT1에서 유래된 DNA polymerase gene을 Escherichia coli로부터 발현시킨 후 정제 과정을 거친 내열성의 Taq DNA polymerase입니다. 

₩65,000
카탈로그 번호
E-2011-CFG

특장점

  • 높은 효율/민감도

    높은 증폭 효율과 template에 대해 높은 민감도를 갖습니다.

  • 최적화된 버퍼 제공

    Enzyme에 최적화된 버퍼를 제공하여 안정적인 PCR 반응을 수행합니다.

  • 재현성

    ISO 9001 품질시스템 하에서 생산되어 각 batch에 대한 균일한 품질의 제품이 공급되기 때문에 재현성 있는 결과를 얻을 수 있습니다.

응용 및 적용

  • Real-time quantification of DNA and cDNA targets using dual probe and dsDNA binding dye
  • Gene expression profiling
  • Microbial & viral pathogen detection

실험 자료

Figure 1. Taq DNA Polymerase의 sensitivity test. 
Taq DNA Polymerase 1 unit으로 DNA Polymerase의 활성을 확인한 실험  (A) human genomic DNA (B) Lambda DNA 
M; 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1; 100 ng template DNA
Lane 2; 10 ng template DNA
Lane 3; 1 ng template DNA
Lane 4; 100 pg template DNA
Lane 5; 10 pg template DNA
Lane 6; 1 pg template DNA
Lane 7; 100 fg template DNA

제품 규격/사양

5' to 3' exonuclease activity

Yes

3' to 5' exonuclease activity

No

3' – A overhang

Yes

Fragment size

Up to 10 kb

제품 구성

10X reaction buffer with (or without) MgCl2: Tris-HCl, KCl, 15 mM MgCl2, pH 9.0

Dilution buffer: 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol, pH 8.0

10 mM dNTPs mix: 2.5 mM of each dNTP

농도

500 units (5 U/μl)

저장 조건

20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0

보관 온도

-20°C

단위(Unit) 정의

One unit is defined as the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72℃.

실험 자료

Figure 1. Taq DNA polymerase serial dilution test.Taq DNA Polymerase를 1/5, 1/10, 1/15, 1/20로 희석하여
human genomic DNA (A), Lambda DNA (B) 를 template로 하여 PCR 반응

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1; 5 U of Taq DNA polymerase
Lane 2; 1 U of Taq DNA polymerase
Lane 3; 0.5 U of Taq DNA polymerase
Lane 4; 0.33 U of Taq DNA polymerase
Lane 5; 0.25 U of Taq DNA polymerase

Figure 2. Taq DNA Polymerase의 long kb DNA 증폭 실험.
5 kb 부터 8 kb 까지 Lambda DNA 20 pg을 template로 이용하여 증폭 test.

M; 1 kb DNA Ladder (Cat. No. D-1040, Bioneer)
Lane 1; 5 kb PCR product
Lane 2; 6 kb PCR product
Lane 3; 7 kb PCR product
Lane 4; 8 kb PCR product


카탈로그 번호 제품명 가격
E-2011 Taq DNA Polymerase, 500 U, 10 mM dNTP, 10X Reaction Buffer with MgCl2 ₩90,000
E-2011-1 Taq DNA Polymerase, 500 U, 10 mM dNTP, 10X Reaction Buffer, 20 mM MgCl2 ₩90,000
E-2011-2 Taq DNA Polymerase, 500 U, 10X Reaction Buffer with MgCl2 ₩65,000
E-2011-3 Taq DNA Polymerase, 500 U, 10X Reaction Buffer, 20 mM MgCl2 ₩65,000
E-2013 Taq DNA Polymerase, 2,000 U, 10 mM dNTP, 10X Reaction Buffer with MgCl2 ₩315,000
E-2013-1 Taq DNA Polymerase, 2,000 U, 10 mM dNTP, 10X Reaction Buffer, 20 mM MgCl2 ₩315,000
E-2013-2 Taq DNA Polymerase, 2,000 U, 10X Reaction Buffer with MgCl2 ₩227,000
E-2013-3 Taq DNA Polymerase, 2,000 U, 10X Reaction Buffer, 20 mM MgCl2 ₩227,000
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