▶ 제품 규격/사양
| 5' to 3' exonuclease activity |
No |
| 3' to 5' exonuclease activity |
No |
| 3' – A overhang |
No |
| Strand displacement |
Yes |
| Fragment size |
Up to 9 kb |
▶ 제품 구성
• 5X reaction buffer: Tris-HCl, KCl, MgCl2(pH 8.1)
• 100 mM DTT
• 10 mM dNTPs mix: 2.5 mM of each dNTP
▶ 농도
10,000 Units (200 U/μl)
▶ 저장 조건
50% glycerol containing 20 mM Tris-HCl (pH 7.6), 150 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 mM (NH4)2SO4, Stabilizers.
▶ 보관 온도
-20°C
▶ 단위(Unit) 정의
One unit is defined as the amount of enzyme required to incorporates 1 nmole of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A) oligo (dT) as template primer.
