AccuPower® Multiplex PCR PreMix

AccuPower® Multiplex PCR PreMix는 한 tube 내에서 한 번 반응으로 20개 target gene의 PCR 산물을 얻을 수 있는 제품입니다. 또한 antibody-based HotStart Toq DNA Polymerase를 적용하여 DNA polymerase가 낮은 온도에서 반응하면서 발생할 수 있는 mis-priming, primer-dimer와 같은 비특이 반응을 줄여줍니다. 본 제품은 multiplex PCR 기술을 이용한 일반적인 assay 및 유전자 진단 관련 genotyping assay에도 적용이 가능하며, cDNA를 이용한 semi-quantitative gene expression 실험에 적합합니다.

₩100,000
카탈로그 번호
K-2111-CFG

Features and Benefits

Multiplex PCR : 
한 tube 내에서 20개의 multiplex PCR 증폭산물 생성할 수 있습니다.

Specificity
HotStart Top DNA polymerase는 반응 전(zero cycle)의 비특이 증폭산물의 생성을 최소화합니다. PCR 온도가 증가하면서 활성화된 HotStart Top DNA polymerase는 정확한 target만을 빠르게 증폭합니다.

Ease-of-Use:
각각의 PCR tube에 HotStart Top DNA polymerase와 PCR 수행에 필요한 모든 구성 성분이 포함되어 있어 template DNA, primer set, D.W.만 넣어 바로 multiplex PCR 반응을 수행할 수 있습니다. 또한 전기 영동 시에 필요한 tracking dye 와 침강제가 포함되어 있어 sample loading buffer를 추가로 넣을 필요가 없으므로 편리하게 사용할 수 있습니다. 

Stability: 
PCR 반응 혼합물에 안정화제를 첨가하여 건조시켜, 장기간 보관하더라도 활성을 안정적으로 유지합니다.

Reproducibility:
ISO 9001 품질 시스템 하에서 one-batch system으로 대량 생산되어 각 batch에 대한 철저한 QC를 거친 후 균일한 품질의 제품으로 공급되기 때문에 사용자가 대량의 시료를 반복적으로 처리할 때 각 tube마다 발생하는 편차 문제를 해결하여 재현성있는 실험 결과를 제공합니다.


Specifications

Enzyme: HotStart Top DNA polymerase
5' to 3' exonuclease: No
3' to 5' exonuclease: No
3' – A overhang: Yes
Fragment Size: Up to 1 kb

Applications

STR analysis
Molecular diagnostic analysis
Qualitative, semi-qualitative gene expression assay
Mutant screening
Transgenic organism analysis
Genotyping assay

Experimental data



figure1

Figure 1. Single PCR and multiplex PCR using AccuPower® Multiplex PCR PreMix

Each lane from left to right indicates the single and multiplex PCR product using AccuPower® Multiplex PCR PreMix.
a) 10 plex multiplex PCR, b) 20plex multiplex PCR
M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, Bioneer)


figure2

Figure 2. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

6-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of Human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2mM MgCl2).
Rxn. Condition: 95°C for 10 min, followed by 35cycles of 30 sec at 95°C, 30 sec at 60°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, Bioneer)


figure3

Figure 3. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

10-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2mM MgCl2).
Rxn. Condition: 95°C for 10 min, followed by 35cycles of 30 sec at 95°C, 30 sec at 65°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer)


figure4

Figure 4. Comparison of amplification quality using Lapchip® between AccuPower® Multiplex PCR PreMix and other supplier's master mix.

a) Virtual gel image. Gel image illustrates data reproducibility of the Lapchip® 90 system.
b) Overlay of expression level using Bioneer's multiplex PCR PreMix and other company's multiplex PCR kit. The electropherogram displays the data between 10 PCR products yield using 10-plex primer sets to illustrate the amplification efficiency.
c) The graph shows the total concentration of PCR products between AccuPower® Multiplex PCR PreMix and other supplier's master mix.


figure5

Figure 5. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

20-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (100 ng ~ 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.)
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2mM MgCl2)
Rxn. Condition: 95°C for 10 min, followed by 35cycles of 30 sec at 95°C, 30 sec at 57°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer) Trademark: 'AccuPower®' is a registered trademark of Bioneer Corporation.




Application Note

• Application Note_Multiplex PCR PreMix PCR_Protozoa Penal


Manual

• Manual_AccuPower® Multiplex PCR PreMix


MSDS

• MSDS_AccuPower® Multiplex PCR PreMix


Brochure

• All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

• EN ISO 13485 - certificate










Cat. No. Product Description Price
K-2111 AccuPower® Multiplex PCR PreMix, 0.2ml thin-wall 8-tube strips with attached cap / 96 tubes, 20 μl reaction ₩100,000
K-2112 AccuPower® Multiplex PCR PreMix, 0.2ml thin-wall 8-tube strips with attached cap / 96 tubes, 50 μl reaction ₩150,000
K-2113 AccuPower® Multiplex PCR PreMix, 0.2ml thin-wall 8-tube strips with attached cap / 480 tubes, 20 μl reaction ₩358,000
K-2114 AccuPower® Multiplex PCR PreMix, 0.2ml thin-wall 8-tube strips with attached cap / 480 tubes, 50 μl reaction ₩537,000




















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