AccuPower® Multiplex PCR PreMix

AccuPower® Multiplex PCR PreMix는 한 tube 내에서 한 번 반응으로 20개 target gene의 PCR 산물을 얻을 수 있는 제품입니다. 또한 antibody-based HotStart Top DNA Polymerase를 적용하여 DNA polymerase가 낮은 온도에서 반응하면서 발생할 수 있는 mis-priming, primer-dimer와 같은 비특이 반응을 줄여줍니다. 본 제품은 multiplex PCR 기술을 이용한 일반적인 assay 및 유전자 진단 관련 genotyping assay에도 적용이 가능하며, cDNA를 이용한 semi-quantitative gene expression 실험에 적합합니다.

Application Note

• Application Note_Multiplex PCR PreMix PCR_Protozoa Penal


₩105,000
카탈로그 번호
K-2111-CFG

특장점

  • Multiplex PCR

    한 tube 내에서 20개의 멀티플렉스 증폭산물을 생성할 수 있습니다.

  • 특이성 & 효율성

    Hotstart Top DNA polymerase를 사용하여 mis-priming 및 primer dimer 등을 불필요한 반응을 최소화 하여 동일 PCR cycle 에서 다른 제품보다 뛰어남 특이도와 증폭효율을 나타내는 제품입니다.

  • 편리성

    정확한 특이도를 통해 불필요한 PCR 조건 선정 과정 없이 template DNA 와 primer 만을 첨가하여 누구나 손쉽게 원하는 타겟을 한 개부터 여러 개를 동시에 증폭 시킬 수 있는 제품입니다.

  • 안정성

    PCR 반응 혼합액에 안정화제가 포함되어 있어 상온배송이 가능하고 solution type 제품보다 안정성면에서 뛰어납니다.

  • 재현성

    ISO 9001 품질 시스템 하에서 대량 생산되어 lot 간 편차가 최소화 되었으며, 반복적인 실험에도 재현성 있는 실험 결과를 제공합니다.

응용 및 적용

• STR analysis

• Molecular diagnostic analysis

• Qualitative, semi-qualitative gene expression assay

• Mutant screening

• Transgenic organism analysis

• Genotyping assay

실험 자료

figure3

Figure. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit.
10-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).

Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl2).
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 65°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer)

제품 규격/사양

Enzyme

HotStart Top DNA polymerase

5' to 3' exonuclease

No

3' to 5' exonuclease

No

3' – A overhang

Yes

Fragment Size

Up to 1 kb

실험 자료

figure1

Figure 1. Single PCR and multiplex PCR using AccuPower® Multiplex PCR PreMix

Each lane from left to right indicates the single and multiplex PCR product using AccuPower® Multiplex PCR PreMix.
a) 10 plex multiplex PCR, b) 20 plex multiplex PCR
M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, Bioneer)



figure2

Figure 2. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

6-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of Human genomic DNA diluents were tested (Lane 1; Human genomic DNA 100 ng, Lane 2; Human genomic DNA 10 ng, Lane 3; Human genomic DNA 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.).
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl2).
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 60°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, Bioneer)



figure4

Figure 3. Comparison of amplification quality using Lapchip® between AccuPower® Multiplex PCR PreMix and other supplier's master mix.

a) Virtual gel image. Gel image illustrates data reproducibility of the Lapchip® 90 system.
b) Overlay of expression level using Bioneer's multiplex PCR PreMix and other company's multiplex PCR kit. The electropherogram displays the data between 10 PCR products yield using 10-plex primer sets to illustrate the amplification efficiency.
c) The graph shows the total concentration of PCR products between AccuPower® Multiplex PCR PreMix and other supplier's master mix.



figure5

Figure 4. Comparison of amplification quality between AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit.

20-plex primers were added into AccuPower® Multiplex PCR PreMix and other supplier's multiplex PCR kit. A series of human genomic DNA diluents were tested (100 ng ~ 1 ng). All data were obtained using Mygenie 96 machine (Applied Bioneer co.)
Supplier Q; Q company multiplex PCR kit, Supplier S; S company multiplex PCR kit, Supplier I; I company Taq polymerase for mutiplex PCR (0.5U, added 2 mM MgCl2)
Rxn. Condition: 95°C for 10 min, followed by 35 cycles of 30 sec at 95°C, 30 sec at 57°C, 60 sec at 72°C M; 25/100 bp Mixed DNA Ladder (cat. No. D-1020, bioneer) Trademark: 'AccuPower®' is a registered trademark of Bioneer Corporation.

Manual

Manual_AccuPower® Multiplex PCR PreMix


MSDS

MSDS_AccuPower® Multiplex PCR PreMix


Brochure

All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate

카탈로그 번호 제품명 가격
K-2111 AccuPower® Multiplex PCR PreMix, 0.2ml thin-wall 8-tube strips with attached cap / 96 tubes, 20 μl reaction ₩105,000
K-2112 AccuPower® Multiplex PCR PreMix, 0.2ml thin-wall 8-tube strips with attached cap / 96 tubes, 50 μl reaction ₩158,000
K-2113 AccuPower® Multiplex PCR PreMix, 0.2ml thin-wall 8-tube strips with attached cap / 480 tubes, 20 μl reaction ₩376,000
K-2114 AccuPower® Multiplex PCR PreMix, 0.2ml thin-wall 8-tube strips with attached cap / 480 tubes, 50 μl reaction ₩564,000
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