AccuPrep® Plasmid Mini Extraction Kit

AccuPrep® Plasmid Mini Extraction Kit는 alkaline lysis method에 기초하여 고순도, 고농도의 plasmid DNA를 추출할 수 있는 제품입니다. 추출된 plasmid DNA는 sequencing, sub-cloning, transformation 등 다양한 실험에 사용될 수 있습니다.

Spin column 방식

₩50,000
카탈로그 번호
K-3030-CFG

특장점

  • 최대 30 µg의 높은 binding capacity를 갖는 silica based DNA binding column 사용

  • endA+ strain을 위한 Endonuclease A denaturation buffer 제공 (PB Buffer)

  • Washing buffer에 ethanol이 첨가되어 있어 편리하게 사용 가능

응용 분야

• Gene cloning

• Sequencing

• Transformation

• Transfection

in-vitro transcription / translation

제품 규격/사양

Feature Specification
Starting culture voulme 1 - 10 ml
Preparation time < 20 min
Elution volume 50 - 100 µl
Expected DNA yield* Up to 20 µg
Expected DNA purity A260/280 > 1.8

* Plasmid 종류에 따라 측정 값의 차가 있을 수 있습니다.

구성품

구성품 K-3030
(200 reaction)
K-3030-1
(50 reaction)
PA1 Buffer (Resuspension) 60 ml X 1 ea 15 ml X 1 ea
P2 Buffer (Lysis) 60 ml X 1 ea 15 ml X 1 ea
PA3 Buffer (Neutralization) 80 ml X 1 ea 20 ml X 1 ea
PB Buffer (Endonuclease A denaturation) 75 ml X 1 ea 20 ml X 1 ea
W2 Buffer (Washing) 80 ml X 2 ea 40 ml X 2 ea
EA Buffer (Elution) 25 ml X 1 ea 15 ml X 1 ea
RNase A powder, lyophilized 6 mg X 1 ea 1.5 mg X 1 ea

AccuPrep® Binding Column-II

200 ea 50 ea
Collection Tube (for filtration) 200 ea 50 ea
사용설명서 1 ea 1 ea

작업 순서

accupower

실험 자료

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Figure 1. Improved yield.

AccuPrep® Plasmid Mini Extraction Kit는 high & low copy number 의 plasmid DNA 모두에서 높은 수율을 나타냅니다.

- Lane B; AccuPrep® Plasmid Mini Extraction Kit
- Lane 2, 3; Competitors




accupower

Figure 2. Restriction enzyme digestion test.

200 ng of extracted plasmid DNA (pBluescript SK(+)) was digested with XbaI. Each enzymes completely recognize its restriction enzyme site and digest.

- Lane M; Molecular weight marker (λDNA/HindIII + EcoRI, Cat. No. D-1070, Bioneer)
- Lane 1; AccuPrep® Plasmid Mini Extraction Kit
- Lane 2, 3; Competitors




accupower

200 ng of extracted plasmid DNA (pBluescript SK(+)) was digested with various restriction enzymes. Each enzymes completely recognize its restriction enzyme site and digest.

- Lane M; Molecular weight marker (λDNA/HindIII + EcoRI, Cat. No., D-1070, Bioneer)
- K; Kpn I            - X; Xho I
- SI; Sal I             - H; Hind III
- E; EcoR I           - P; Pst I
- Sm; Sma I        - B; Bam HI
- S; Spe I             - Xb; Xba I
- N; Not I            - Sc; Sac I


카탈로그 번호 제품명 가격
K-3030 AccuPrep® Plasmid Extraction Kit 200 reactions ₩100,000
K-3030-1 AccuPrep® Plasmid Extraction Kit 50 reactions ₩50,000
KB-0101 RNase A Powder, 6 mg ₩30,000
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