AccuPrep® Stool DNA Extraction Kit (100 reactions)

AccuPrep® Stool DNA Extraction Kit는 인간을 비롯한 다양한 동물의 fresh 혹은 frozen stool 로부터 다양한 PCR 저해물질을 제거하여 고순도의 genomic DNA를 추출할 수 있는 제품입니다.

Spin column 방식

₩133,000
카탈로그 번호
K-3036

특장점

  • 인간 및 다양한 동물들의 stool로부터 고순도의 genomic DNA 추출 가능

  • 최대 30 µg의 높은 binding capacity를 갖는 silica based DNA binding column 사용

  • 다양한 stool sample에 최적화된 Stool Lysis buffer (SL Buffer)를 사용하여 다양한 PCR 저해물질 (heparin, bilirubin 등)이 완벽히 제거된 genomic DNA 추출 가능

  • Washing buffer에 ethanol이 첨가되어 있어 편리하게 사용 가능

응용 분야

• PCR

• Real-time PCR

• SNP genotyping

• Pathogen characterization

제품 규격/사양

시료 종류 시료 양 DNA 수율
Stool 100 mg 2 - 5 μg

* 시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.

구성품

구성품 K-3036
SL Buffer (Stool Lysis) 50 ml x 1ea
SB Buffer (Binding) 50 ml x 1ea
WA1 Buffer (1st Washing) 40 ml x 1ea
W2 Buffer (2nd Washing) 80 ml x 1ea
EA Buffer (Elution) 25 ml x 1ea
Proteinase K, lyophilized 25 mg x 2 ea
AccuPrep® Binding Column-I 100 ea
Collection Tube (for filtration) 100 ea
1.5 ml tubes (for elution) 100 ea
Quick Manual 1 ea

작업 순서

Stool_DNA_Extraction_Ki

실험 자료

Stool_DNA_Extraction_Ki

Figure 1. Gel electrophoresis of stool genomic DNA extracted by AccuPrep® Stool DNA Extraction Kit

- Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (D-1070, Bioneer)
- Lane M2; Molecular weight marker, 1 kb ladder (D-1040, Bioneer)
- Lane 1~3; DNA purified with Bioneer's AccuPrep® Stool DNA Extraction Kit.
100 ng of intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)  

Stool_DNA_Extraction_Ki

Figure 2. PCR results of H. pylori DNA extracted with AccuPrep® Stool DNA Extraction Kit

- Lane M; Molecular weight marker, 100 bp ladder (D-1030, Bioneer)
- Lane 1~4; PCR results for H. pylori detection

제품 규격/사양

시료 종류 시료 양 DNA 수율
Stool 100 mg 2 - 5 μg

* 시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.

구성품

구성품 K-3036
SL Buffer (Stool Lysis) 50 ml x 1ea
SB Buffer (Binding) 50 ml x 1ea
WA1 Buffer (1st Washing) 40 ml x 1ea
W2 Buffer (2nd Washing) 80 ml x 1ea
EA Buffer (Elution) 25 ml x 1ea
Proteinase K, lyophilized 25 mg x 2 ea
AccuPrep® Binding Column-I 100 ea
Collection Tube (for filtration) 100 ea
1.5 ml tubes (for elution) 100 ea
Quick Manual 1 ea

작업 순서

Stool_DNA_Extraction_Ki

실험 자료

Stool_DNA_Extraction_Ki

Figure 1. Gel electrophoresis of stool genomic DNA extracted by AccuPrep® Stool DNA Extraction Kit

- Lane M1; Molecular weight marker, λ DNA/EcoR l + Hind lll markers (D-1070, Bioneer)
- Lane M2; Molecular weight marker, 1 kb ladder (D-1040, Bioneer)
- Lane 1~3; DNA purified with Bioneer's AccuPrep® Stool DNA Extraction Kit.
100 ng of intact DNA (left) and Hind III (E-1721, Bioneer) enzyme digested DNA (right)  

Stool_DNA_Extraction_Ki

Figure 2. PCR results of H. pylori DNA extracted with AccuPrep® Stool DNA Extraction Kit

- Lane M; Molecular weight marker, 100 bp ladder (D-1030, Bioneer)
- Lane 1~4; PCR results for H. pylori detection


카탈로그 번호 제품명 가격
K-3036 AccuPrep® Stool DNA Extraction Kit,100 reactions ₩133,000
KB-0111 Proteinase K Powder, 25 mg X 2 ₩70,000
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