특장점
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배양 세포, 동물 조직, 식물 조직 등 다양한 샘플 시료로부터 고순도, 고수율의 RNA 추출 가능
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최대 120 µg의 높은 binding capacity를 갖는 silica based RNA binding column 사용
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Column을 개별 멸균 포장하여 오염을 최소화
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Washing buffer에 ethanol이 첨가되어 있어 편리하게 사용 가능
응용 분야
• cDNA synthesis
• RT-PCR
• Quantative real-time RT-PCR
• Northern blot analysis
▶ 제품 규격/사양
| Column loading volume |
800 μl |
| Max binding capacity |
~ 120 μg |
| Elution volume |
30 μl - 100 μl |
| Preparation time |
~ 20 min |
| 시료 종류 |
시료 양 |
RNA 수율 |
순도* |
| Cultured cells |
104 – 108 cells |
15 – 20 μg |
A260/280 > 2.0
A260/230 > 1.7 |
| Mammalian tissue |
25 – 50 mg |
10 - 60 μg |
| Plant tissue |
100 mg |
30 - 60 μg |
*시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.
▶ 구성품
| 구성품 |
K-3141
(50 reaction) |
K-3140
(100 reaction) |
| RB Buffer (RNA Binding) |
30 ml x 1 ea |
60 ml x 1 ea |
| RWA1 Buffer (1st Washing) |
40 ml x 1 ea |
80 ml x 1 ea |
| RWA2 Buffer (2nd Washing) |
70 ml x 1 ea |
70 ml x 2 ea |
| ER Buffer (Elution) |
10 ml x 1 ea |
20 ml x 1 ea |
| AccuPrep® Binding Column-Ⅲ |
50 ea |
100 ea |
| 1.5 ml Tube (for collection) |
50 ea |
100 ea |
| 사용설명서 |
1 ea |
1 ea |
▶ 실험 자료
Figure 1. Cultured cell RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 1×106 of HeLa cell. DNase was not treated.
1: Bioneer, 2: Company Q
Figure 2. Plant RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 100 mg of Brassica oleracea var. italica. DNase was not treated.
1: Bioneer, 2: Company Q
Figure 3. Animal tissue RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 20 mg of Mus musculus liver. DNase was not treated.
1: Bioneer, 2: Company Q
