MagListo™ 5M Tissue Total RNA Extraction Kit (100 reactions)

MagListo™ 5M Tissue Total RNA Extraction Kit 은 Magnetic Nanobead와 MagListo™ Magnetic Separation Rack을 이용하여 다양한 조직과 세포에서 total RNA 를 고순도로 빠르게 추출할 수 있는 제품입니다. 자성분리방식으로 세포 분쇄물에서 total RNA를 분리하고 농축 및 정제하는 과정을 거치기 때문에 원심분리방법에 비해 빠르고 간편하게 total RNA를 분리할 수 있습니다. 본 제품은 한 kit를 사용하여 mini, midi scale로 total RNA를 추출할 수 있어 별도의 kit를 구매할 필요가 없으며, midi prep을 위한 vacuum system이나 air pressure system을 구비할 필요가 없어 실험비용을 절감할 수 있습니다.

₩349,000
In stock
SKU
K-3613

Features and Benefits

  • MagListo™ Extraction Kit와 MagListo™ Magnetic Separation Rack 외의 고가 추가 장비 불필요
  • Animal tissue, cultured cell 에서 고순도 total RNA 추출
  • Mini, midi scale의 kit를 따로 구매할 필요 없이 한 kit로 RNA 추출
  • Magnetic NanoBead를 이용하여 Mini-10분, Midi-15분만에 total RNA 추출

Kit Components

Reagents *K-3613
Buffer ① (Binding) 25 ml x 2 ea
Buffer ② (1st Washing) 100 ml x 1 ea
Buffer ③ (2nd Washing) 100 ml x 1 ea
Buffer ④ (3rd Washing) 120 ml x 1 ea
Buffer ⑤ (Elution) 25 ml x 1 ea
Magnetic Nano Bead - RNA 1.8 ml x 6 ea
  *mini-100 rxn, midi-10 rxn

Specifications

  mini scale midi scale
Starting sample ≤ 30 mg tissue
3 x 106 cells
≤ 200 mg tissue
1 x 106 cells
Extraction time < 10 min < 15 min
Minimum elution volume 50 µl 500 µl
Expected RNA yield Up to 100 μg Up to 500 μg
Expected purity A260/280 > 1.9
  
 *RNA content can vary greatly depending on tissue types.


Typical Yields of Total RNA

Tissue type (10 mg) Yield (µg)
Kidney 20 - 30
Liver 40 - 60
Spleen 20 - 40
Thymus 30 - 50
Lung 10 - 20

Applications

RT-PCR and real-time RT-PCR
cDNA synthesis
Northern, dot, and slot blot analyses
Primer extension
Microarrays


Experimental Data

Prep kit No. Yield (μg) 260/280 260/230
MagListo 1 58.6 2.09 2.03
2 60.3 2.09 1.95
3 55.7 2.08 2.02
4 58.7 2.09 1.97
Competitor 5 36.8 2.04 1.76
6 38.3 2.05 1.78
7 34.5 2.03 1.91
8 37.9 2.05 1.48

Figure 1. Comparison of total RNA purified from liver tissue (10 mg) with MagListo™ 5M Tissue Total RNA Extraction Kit and competitor’s kit (spin column type).


Prep kit No. Yield (μg) 260/280 260/230
MagListo 1 30.8 2.02 2.02
2 34.6 2.02 2.03
3 33.7 2.03 1.95
4 35.2 2.06 1.91
Competitor 5 27.8 2.03 1.95
6 26.7 2.03 1.87
7 28.3 2.03 1.76
8 27.6 2.04 1.75

Figure 2. Comparison of total RNA purified from kidney tissue (10 mg) with MagListo™ 5M Tissue Total RNA Extraction Kit and competitor’s kit (spin column type).



Prep kit No. Yield (μg) 260/280 260/230
MagListo 1 24.5 2.06 1.78
2 25.2 2.07 1.88
3 22.8 2.06 1.94
4 21.6 2.06 1.87
5 22.1 2.07 1.92
6 22.6 2.06 1.82

Figure 3. Electrophoresis data of total RNA purified from Hela cells using MagListo™ 5M Tissue Total RNA Extraction Kit.



Prep kit RNA Quality Score 5S Area 5S %Total 18S Area 18S %Total 28S Area 28S %Total rRNA Area Ratio
[28S/18S]
rRNA Height Ratio
[28S/18S]
MagListo 10.0 2.05 0.01 25.27 0.15 96.37 0.59 3.81 1.90
10.0 2.06 0.01 29.47 0.18 111.78 0.68 3.79 1.90
10.0 1.54 0.02 16.09 0.16 54.10 0.55 3.36 1.73
10.0 1.79 0.01 23.08 0.14 80.96 0.50 3.51 1.80
Competitor 10.0 1.96 0.01 31.72 0.15 119.04 0.57 3.75 1.90
10.0 1.77 0.01 23.25 0.16 102.63 0.70 4.41 2.06
10.0 1.44 0.01 20.33 0.15 84.37 0.61 4.15 2.06
10.0 1.44 0.01 25.22 0.14 99.41 0.54 3.94 1.93

Table1. Result of capillary electrophoresis of total RNA extraction from Hela cell using LabChip.



N: Number of sample

Figure 4. Comparison of RNA extraction time between MagListo™ 5M Tissue Total RNA Extraction Kit and competitor’s kit (spin column type).


Manuals

Manual_MagListo™ 5M Tussue total RNA Extraction Kit
Quickmanual_MagListo™ 5M Tussue total RNA Extraction Kit


MSDS

MSDS_MagListo™ 5M Tissue total RNA Extraction Kit


Brochure

MagListo™ Tissue total RNA Extraction Kit - Brochure Download
MagListo™ Flyer -


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.
• ISO 9001 - certificate










Cat. No. Product Description Price
K-3613 MagListo™ 5M Tissue Total RNA Extraction Kit, 100 rxns in mini ₩349,000

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