MagListo™ 5M Tissue Total RNA Extraction Kit (100 reactions)

MagListo™ 5M Tissue Total RNA Extraction Kit 은 Magnetic Nanobead가 적용된 제품으로서  MagListo™ Magnetic Separation Rack을 이용하여 다양한 동물조직과 배양세포로부터 total RNA를 빠르게 추출할 수 있는 제품입니다. 추출된 RNA는 RT-PCR, Quantitative real time RT-PCR, microarray analysis 등 다양한 실험에 사용할 수 있습니다.

Magnetic bead 방식

₩349,000
카탈로그 번호
K-3613

특장점

  • 다양한 동물 조직과 배양세포로부터 Total RNA 추출 가능

  • Mini, midi scale을 하나의 kit로 사용 가능 (Mini-10분, Midi-15분)

  • Extraction Kit와 MagListo™ Magnetic Separation Rack 외의 고가 추가 장비 불필요

응용 분야

• RT-PCR and real-time RT-PCR

• cDNA synthesis

• Northern blot analysis

• Primer extension

• Microarrays

제품 규격/사양

Scale Mini Midi
Starting sample ≤ 30 mg tissue
3 x 106 cells
≤ 200 mg tissue
1 x 106 cells
Extraction time < 10 min < 15 min
Minimum elution volume 50 µl 500 µl
Expected RNA yield Up to 100 μg Up to 500 μg
Expected purity A260/280 > 1.9

*시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.


Tissue type (10 mg) Yield (µg)
Kidney 20 - 30
Liver 40 - 60
Spleen 20 - 40
Thymus 30 - 50
Lung 10 - 20

구성품

구성품 K-3613*
Buffer ① (Binding) 25 ml x 2 ea
Buffer ② (1st Washing) 100 ml x 1 ea
Buffer ③ (2nd Washing) 100 ml x 1 ea
Buffer ④ (3rd Washing) 120 ml x 1 ea
Buffer ⑤ (Elution) 25 ml x 1 ea
Magnetic Nanobead 1.8 ml x 6 ea
사용설명서 1 ea

*mini-100 rxn, midi-10 rxn

실험 자료

Prep kit No. Yield (μg) 260/280 260/230
MagListo 1 58.6 2.09 2.03
2 60.3 2.09 1.95
3 55.7 2.08 2.02
4 58.7 2.09 1.97
Competitor 5 36.8 2.04 1.76
6 38.3 2.05 1.78
7 34.5 2.03 1.91
8 37.9 2.05 1.48

Figure 1. Comparison of total RNA purified from liver tissue (10 mg) with MagListo™ 5M Tissue Total RNA Extraction Kit and competitor’s kit (spin column type).


Prep kit No. Yield (μg) 260/280 260/230
MagListo 1 30.8 2.02 2.02
2 34.6 2.02 2.03
3 33.7 2.03 1.95
4 35.2 2.06 1.91
Competitor 5 27.8 2.03 1.95
6 26.7 2.03 1.87
7 28.3 2.03 1.76
8 27.6 2.04 1.75

Figure 2. Comparison of total RNA purified from kidney tissue (10 mg) with MagListo™ 5M Tissue Total RNA Extraction Kit and competitor’s kit (spin column type).



Prep kit No. Yield (μg) 260/280 260/230
MagListo 1 24.5 2.06 1.78
2 25.2 2.07 1.88
3 22.8 2.06 1.94
4 21.6 2.06 1.87
5 22.1 2.07 1.92
6 22.6 2.06 1.82

Figure 3. Electrophoresis data of total RNA purified from Hela cells using MagListo™ 5M Tissue Total RNA Extraction Kit.


Prep kit RNA Quality Score 5S Area 5S %Total 18S Area 18S %Total 28S Area 28S %Total rRNA Area Ratio
[28S/18S]
rRNA Height Ratio
[28S/18S]
MagListo 10.0 2.05 0.01 25.27 0.15 96.37 0.59 3.81 1.90
10.0 2.06 0.01 29.47 0.18 111.78 0.68 3.79 1.90
10.0 1.54 0.02 16.09 0.16 54.10 0.55 3.36 1.73
10.0 1.79 0.01 23.08 0.14 80.96 0.50 3.51 1.80
Competitor 10.0 1.96 0.01 31.72 0.15 119.04 0.57 3.75 1.90
10.0 1.77 0.01 23.25 0.16 102.63 0.70 4.41 2.06
10.0 1.44 0.01 20.33 0.15 84.37 0.61 4.15 2.06
10.0 1.44 0.01 25.22 0.14 99.41 0.54 3.94 1.93

Table1. Result of capillary electrophoresis of total RNA extraction from Hela cell using LabChip.




Figure 4. Comparison of RNA extraction time between MagListo™ 5M Tissue Total RNA Extraction Kit and competitor’s kit (spin column type).

N: Number of sample

Manual

Manual_MagListo™ 5M Tussue total RNA Extraction Kit

Quickmanual_MagListo™ 5M Tussue total RNA Extraction Kit


MSDS

MSDS_MagListo™ 5M Tissue total RNA Extraction Kit


Brochure

MagListo™ nucleic acid extraction kit series


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate


카탈로그 번호 제품명 가격
K-3613 MagListo™ 5M Tissue Total RNA Extraction Kit, 100 rxns in mini ₩349,000

관련 제품

카탈로그 번호 제품명 가격
1750-00 1 ml tube with 8-cap strip, 100 ea / pk ₩35,000
50015 15 ml tube, 500 ea / pk ₩79,200
50050 50 ml tube, 500 ea / pk ₩107,800
HT-15-NG Graduated 1.5 ml Microcentrifuge Tubes, Natural, 500 ea ₩19,200
HT-20-NG Standard 2.0 ml Microcentrifuge Tubes, Natural, 500 ea ₩21,600
TM-1010 MagListo™-2 Magnetic Separation Rack, 2 ml tube x 8 holes ₩255,000
TM-1020 MagListo™-15 Magnetic Separation Rack, 15 ml tube x 6 holes ₩455,000
TM-1030 MagListo™-50 Magnetic Separation Rack, 50 ml tube x 3 holes ₩455,000
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