AccuNanoBead™ Protein G Magnetic Nanobeads

AccuNanoBead™ Protein G Magnetic Nanobeads 는 고순도(> 95%) Protein G가 coating된 silica magnetic nano bead입니다. 이러한 bead는 특이적으로 항체를 인지하여 항체정제, immunoprecipitation, 항원-항체 상호작용 연구, 단백질 복합체 연구, cell separation 등에 응용할 수 있습니다.
₩152,000
카탈로그 번호
TA-1021-1-CFG

AccuNanoBead™ Protein G Magnetic Nanobeads 는 고순도(> 95%) Protein G가 coating된 silica magnetic nano bead입니다. 이러한 bead는 특이적으로 항체를 인지하여 항체정제, immunoprecipitation, 항원-항체 상호작용 연구, 단백질 복합체 연구, cell separation 등에 응용할 수 있습니다.

Features and Benefits

 Fast Binding: 강력한 자성력은 비드의 빠른 회수율로 실험을 보다 빠르고 손실 없이 진행할 수 있습니다.
 Large Surface Area: 평균직경 400 nm의 magnetic nanobeads는 표면적이 넓고 결합력이 우수하여 높은
                                     binding capacity를 보장합니다.
 Specificity:균일한 구형의 nanobead로 비특이적 결합을 감소시켰습니다.
 Components

Kit components Cat. No. TA-1021-1
Protein G magnetic nano beads 1mL X 1ea (4 %)
Manual 1 ea

















Specifications

Feature Specification
Matrix Silica-coated Fe3O4
Concentration 40 mg/ml (4% suspension)
Max. Binding Capacity > 30 μg Human IgG/mg beads sample
Storage and Stability Beads can be stored at 2~8°C until the expiration date. Do not freeze and vortex vigorously.

Application

Antibody purification, Antigen purification, Immunoprecipitation, Protein-protein interaction, and Cell separation etc.

Experimental Data


figure1

Figure 1. Comparison of protein binding efficiency of AccuNanoBead™ Protein G Magnetic Nanobeads with a competitor.
A) Eluted fraction after binding of Human IgG followed by washing through Protein G magnetic bead from Bioneer (AccuNanoBead™) or from a competitor L. Upper and lower bands are heavy (HC) and light chain (LC) of purified Human IgG in SDS-PAGE gel, respectively.
B) Comparison of binding capacity in Bioneer’s AccuNanoBead™ with competitor’s. Amount of bound IgG is quantified by UV-spectrophotometer. All reaction is carried out for 1 hour at room temperature. Human IgG is purchased from Sigma (Cat. no. I4506).
M: protein marker (Bioneer, Cat. No. D-2020), Bioneer: AccuNanoBead™ Protein G Magnetic Nanobeads.



figure2

Figure 2. IgG purification of AccuNanoBead™ Protein G Magnetic Nanobeads from human whole blood
IgG was purified from human whole blood. Arrows point to heavy and light chains of IgG, respectively.
M: protein marker, W: human whole blood, U: unbound fraction, E1 & 2: elution fraction 1 & 2. 


Manual

AccuNanoBead™ Protein G Magnetic Nanobeads


MSDS

• MSDS_AccuNanoBead™ Protein G Magnetic Nanobeads


Brochure

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Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate


Cat. No. Product Description Price Add to Cart
TA-1021-1 AccuNanoBead™ Protein G Magnetic Nanobeads, size 400nm, 40mg/1ml ₩152,000 add cart
TA-1021-2 AccuNanoBead™ Protein G Magnetic Nanobeads, size 400nm, 200mg/10ml ₩522,000 add cart
TA-1021-3 AccuNanoBead™ Protein G Magnetic Nanobeads, size 400nm, 500mg/25ml ₩990,000 add cart

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