AccuPrep® Plasmid Mini Extraction Kit

AccuPrep® Plasmid Mini Extraction Kit는 Birnboim의 alkaline lysis method에 기초하여 고순도, 고농도의 plasmid DNA를 추출할 수 있는 제품입니다. 본 제품은 높은 DNA binding capacity를 가지고 있는 silica based filter를 사용하여 큰 size의 plasmid DNA나 많은 양의 plasmid DNA 추출에 매우 효과적입니다. 추출된 plasmid DNA는 sequencing, sub-cloning, transformation 등 다양한 실험에 사용될 수 있습니다.

₩50,000
카탈로그 번호
K-3030-CFG

Features and Benefits

  • 높은 binding capacity를 갖는 silica based DNA binding column 사용
  • endA+ strain을 위한 Endonuclease A denaturation buffer 제공 (PB Buffer)


Components K-3030
(200 reaction)
K-3030-1
(50 reaction)
PA1 Buffer 60 ml X 1 ea 15 ml X 1 ea
P2 Buffer  60 ml X 1 ea 15 ml X 1 ea
PA3 Buffer 80 ml X 1 ea 20 ml X 1 ea
PB Buffer 75 ml X 1 ea 20 ml X 1 ea
W2 Buffer 80 ml X 2 ea 40 ml X 2 ea
EA Buffer 25 ml X 1 ea 15 ml X 1 ea
RNase A 6 mg X 1 ea 1.5 mg X 1 ea
Binding Column 200 ea 50 ea
Collection tubes 200 ea 50 ea
Quick Manual 1 ea 1 ea




Specifications

Starting culture volume: 1 ml ~ 10 ml
Column binding capacity: > 20 µg
Elution volume: 50 – 100 µl
Expected DNA yield: up to 20 µg
Preparation time: ~ 20  min


Applications

Sub-cloning
Sequencing
Transformation
Trasnfection
in-vitro transcription/translation


Procedure

accupower


Experimental Data

Figure 1. Improved yield.

accupower

AccuPrep® Plasmid Mini Extraction Kit는 high & low copy number 의 plasmid DNA 모두에서 높은 수율을 나타냅니다.
Lane B; AccuPrep® Plasmid Mini Extraction Kit, Lane 2, 3; Competitors

Figure 2. Restriction enzyme digestion test.

accupower
200 ng of extracted plasmid DNA (pBluescript SK(+)) was digested with XbaI. Each enzymes completely recognize its restriction enzyme site and digest.
Lane M; Molecular weight marker (λDNA/HindIII + EcoRI, Cat. No. D-1070, Bioneer)
Lane 1; AccuPrep® Plasmid Mini Extraction Kit
Lane 2, 3; Competitors


accupower
200 ng of extracted plasmid DNA (pBluescript SK(+)) was digested with various restriction enzymes. Each enzymes completely recognize its restriction enzyme site and digest.
Lane M; Molecular weight marker (λDNA/HindIII + EcoRI, Cat. No., D-1070, Bioneer)
K; Kpn I, X; Xho I, SI; Sal I, H; Hind III, E; EcoR I, P; Pst I, Sm; Sma I, B; Bam HI, S; Spe I, Xb; Xba I, N; Not I, Sc;Sac I




Manual

• Manual_AccuPrep Plasmid Mini Extraction Kit


Brochure

AccuPrep® nucleic acid extraction kit series


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate


















Cat. No. Product Description Price
K-3030 AccuPrep® Plasmid Extraction Kit 200 reactions ₩100,000
K-3030-1 AccuPrep® Plasmid Extraction Kit 50 reactions ₩50,000
KB-0101 RNase A Powder, 6 mg ₩30,000
























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