AccuPrep® Universal RNA Extraction Kit

AccuPrep® Universal RNA Extraction Kit는 cultured cell, mammalian tissue, plant tissue 로부터 RNA를 빠르게 추출할 수 있는 제품입니다. 추출된 RNA는 RT-PCR, quantitative real time RT-PCR, microarray analysis 등 다양한 실험에 사용할 수 있습니다.

Spin column 방식

₩170,000
카탈로그 번호
K-3140-CFG

특장점

  • 배양 세포, 동물 조직, 식물 조직 등 다양한 샘플 시료로부터 고순도, 고수율의 RNA 추출 가능

  • 최대 120 µg의 높은 binding capacity를 갖는 silica based RNA binding column 사용

  • Column을 개별 멸균 포장하여 오염을 최소화

  • Washing buffer에 ethanol이 첨가되어 있어 편리하게 사용 가능

응용 분야

• cDNA synthesis

• RT-PCR

• Quantative real-time RT-PCR

• Northern blot analysis

제품 규격/사양

Column loading volume 800 μl
Max binding capacity ~ 120  μg
Elution volume 30  μl - 100  μl
Preparation time ~ 20 min

시료 종류 시료 양 RNA 수율 순도*
Cultured cells 104 – 108 cells 15 – 20 μg A260/280 > 2.0
A260/230 > 1.7
Mammalian tissue 25 – 50 mg 10 - 60 μg
Plant tissue 100 mg 30 - 60 μg

*시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.

구성품

구성품 K-3141
(50 reaction)
K-3140
(100 reaction)
RB Buffer (RNA Binding) 30 ml x 1 ea 60 ml x 1 ea
RWA1 Buffer (1st Washing) 40 ml x 1 ea 80 ml x 1 ea
RWA2 Buffer (2nd Washing) 70 ml x 1 ea 70 ml x 2 ea
ER Buffer (Elution) 10 ml x 1 ea 20 ml x 1 ea
AccuPrep® Binding Column-Ⅲ 50 ea 100 ea
1.5 ml Tube (for collection) 50 ea 100 ea
사용설명서 1 ea 1 ea

실험 자료


Figure 1. Cultured cell RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 1×106 of HeLa cell. DNase was not treated.
1: Bioneer, 2: Company Q

Figure 2. Plant RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 100 mg of Brassica oleracea var. italica. DNase was not treated.
1: Bioneer, 2: Company Q

Figure 3. Animal tissue RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 20 mg of Mus musculus liver. DNase was not treated.
1: Bioneer, 2: Company Q

Manuals

Manual_AccuPrep® Universal RNA Extraction Kit

 

Brochure

AccuPrep® nucleic acid extraction kit series

 

MSDS

• MSDS AccuPrep® Universal RNA Extraction Kit

 

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2000 - certificate

카탈로그 번호 제품명 가격
K-3140 AccuPrep® Universal RNA Extraction Kit, 100 reactions  ₩300,000
K-3141 AccuPrep® Universal RNA Extraction Kit, 50 reactions ₩170,000
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