AccuPrep® Universal RNA Extraction Kit

AccuPrep® Universal RNA Extraction Kit는 cultured cell, mammalian tissue, plant tissue 로부터 RNA를 빠르게 추출할 수 있는 제품입니다. 본 제품은 높은 RNA binding capacity를 갖는 silica filter가 사용된 spin column 방식의 제품으로, 다양한 시료에 최적화된 lysis (binding) buffer를 비롯하여 washing buffer 및 elution buffer로 구성되어 있습니다. AccuPrep® Universal RNA Extraction Kit에 의해 추출된 RNA는 RT-PCR, quantitative real time RT-PCR 등에 사용할 수 있습니다.
₩170,000
카탈로그 번호
K-3140-CFG

Features and Benefits

- 배양 세포, 동물 조직, 식물 조직 등 다양한 샘플 시료로부터 고순도, 고수율의 RNA 추출 가능

- 높은 binding capacity를 갖는 silica based RNA binding column 사용

- 유기용매를 사용하지 않아 안전함

 

Reagents

K-3141

K-3140

RB Buffer

30 ml x 1 ea

60 ml x 1 ea

RWA1 Buffer

40 ml x 1 ea

80 ml x 1 ea

RWA2 Buffer

70 ml x 1 ea

70 ml x 2 ea

ER Buffer

10 ml x 1 ea

20 ml x 1 ea

AccuPrep® Binding Column-Ⅲ

50 ea

100 ea

1.5 ml Tube (for collection)

50 ea

100 ea

Quick Manual

1

1

 

Specifications

 

Amount

Typical yield

Cultured cells

104 – 108 cells

15 – 20 μg

Mammalian tissue

25 – 50 mg

10 - 60 μg

Plant tissue

100 mg

30 - 60 μg

Expected purity

A260/280 > 1.9

*RNA content can vary greatly between cell types.

 

Applications

cDNA synthesis
RT-PCR
Quantative real-time RT-PCR
Northern blot analysis

 

Experimental Data


Figure 1. Cultured cell RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 1×106 of HeLa cell. DNase was not treated.
1: Bioneer, 2: Company Q

Figure 2. Plant RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 100 mg of Brassica oleracea var. italica. DNase was not treated.
1: Bioneer, 2: Company Q

Figure 3. Animal tissue RNA extraction using AccuPrep® Universal RNA Extraction Kit
RNA extraction from 20 mg of Mus musculus liver. DNase was not treated.
1: Bioneer, 2: Company Q

Manuals

Manual_AccuPrep® Universal RNA Extraction Kit

 

Brochure

AccuPrep® nucleic acid extraction kit series

MSDS

MSDS AccuPrep® Universal RNA Extraction Kit

 

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001:2000 - certificate

Cat. No. Product Description Price
K-3140 AccuPrep® Universal RNA Extraction Kit, 100 reactions  ₩300,000
K-3141 AccuPrep® Universal RNA Extraction Kit, 50 reactions ₩170,000

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