ExiProgen™ Dialysis Kit, 16 Rxn

바이오니아의 ExiProgen™ 장비를 이용하여 단백질을 전자동으로 dialysis 할 수 있는 kit 입니다. 교환 하고자 하는 buffer를 kit 내의 카트리지에 넣어준 후 ExiProgen™에 장착하고, 이미 설치되어 있는 protocol를 가동시키면 buffer 교환이 전자동으로 이루어지며, 최종 목적 단백질을 원하는 buffer에 용해된 상태로 회수할 수 있습니다.
₩99,000
카탈로그 번호
K-7240

Features and Benefits


Efficiency

적은 양의 buffer를 이용하여 원하는 buffer로 교환할 수 있습니다.


Convenience 

추가적인 냉장 시설 없이 저온에서 buffer를 교환할 수 있습니다.


Fully Automatic

시료 로딩 후 전자동으로 모든 과정이 진행됩니다.


Parallel Processing

16종의 단백질의 buffer를 동시에 교환할 수 있습니다.

Kit Contents and Storage Condition

 

ExiProgen™ Dialysis Kit (K-7240)

96 well plate

2 ea (Cartridge ①, ②)

Store at 4 - 8

Disposable filter tip

2 pack (8 ea/pack)

Dialysis tube in 2 ml tube

1 pack (16 ea/pack)

Protection cover

1 ea

Manual

1 ea

 

Application

단백질 buffer 교환, 단백질 최적 buffer screening

 

Experimental Data

Protocol number in ExiProgen

No. 913 : Storage_12

No. 914 : Storage_6

Buffer exchange

700 µl * 24 times

700 µl * 12 times

Cartridge

2 ea (1.4 ml * 12 well)

1 ea (1.4 ml * 6 well)

 

 

Figure.1. Comparison of dialysis efficiency according to different protocol using 500 mM imidazole.

Ⅰ: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 0.2 mM EDTA, 0.05% NaN3,

Ⅱ: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 0.2 mM EDTA, 0.05% NaN3, 10% Glycerol

Ⅲ: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 0.2 mM EDTA, 0.05% NaN3, 20% Glycerol

Ⅳ: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 0.2 mM EDTA, 0.05% NaN3, 30% Glycerol

Ⅴ: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 0.2 mM EDTA, 0.05% NaN3, 40% Glycerol

Ⅵ: 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 0.2 mM EDTA, 0.05% NaN3, 50% Glycerol

Ⅶ: PBS

Ⅷ: 100 mM HEPES (pH7.6), 100 mM NaCl, 0.2 mM EDTA, 0.05% NaN3

 

 

 

 

Figure. 2. Comparison of dialysis efficiency between manual and automation using 500 mM imidazole.

Buffer Ⅰ; 50 mM Tris-Cl (pH7.6), 100 mM NaCl, 50% Glycerol. Buffer Ⅱ; PBS

Manual dialysis : Dialyzed with dialysis buffer of 100-fold sample volume at 4 ° C.           

 Exiprogen™ : no. 913 : Storage_12

Manual

• Manual Exiprogen™ Dialysis Kit_

MSDS

 

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

• EN ISO 13485 - certificate


Cat. no.

Product Description

Price

K-7240

ExiProgen™ Dialysis Kit, 16 rxn

99,000

 

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