EcoQprep™ mRNA Kit

Table 1. Yield and purity of total RNA and mRNA purified from various samples.

Total RNA was purified using the AccuPrep^® Universal RNA Extraction Kit, and polyadenylated mRNA was purified using the EcoQprep™ mRNA Kit.
RNA yield (µg) and purity (A₂₆₀/A₂₈₀) were measured with a spectrophotometer. The mRNA ratio in total RNA (%) was calculated from the proportion of mRNA yield relative to total RNA yield. Samples tested included HeLa cells (2 × 10⁵ cells), mouse liver (10 mg), mouse brain (10 mg), and broccoli (40 mg).

Total RNA	mRNA	Number of HeLa Cells	Total RNA	mRNA
		2 x 105	20.98	20.60
		2 x 104	25.04	24.57
		2 x 103	29.05	28.13
		2 x 102	24.85	31.90
		2 x 101	UD	35.01
		2 x 100	UD	37.51

Figure 1. Comparison of real-time two-step RT-qPCR amplification of HPRT1 from HeLa cells using total RNA and mRNA purification kits.

HeLa cells (2 × 10⁵ – 2 × 10⁰ cells) were used as input samples. Total RNA was purified with the AccuPrep^® Universal RNA Extraction Kit, and polyadenylated mRNA was purified with the EcoQprep™ mRNA Kit. Amplification of the HPRT1 gene was performed by two-step RT-qPCR.
[Total RNA] Amplification curves obtained from total RNA.
[mRNA] Amplification curves obtained from mRNA.
[Table] Ct values of HPRT1 detected from serial dilutions of HeLa cells. UD = undetermined.