▶ 제품 규격/사양
Scale |
Micro |
Mini
|
Midi
|
Maxi
|
Whole blood |
Amount of Starting Sample |
100 μL |
200 μL |
2 mL |
4 mL |
Typical DNA Yield [μg] |
< 5 μg |
< 10 μg |
< 80 μg |
< 150 μg |
Cultured cells |
Amount of Starting Sample |
~ 1 x 104 cells |
~ 1 x 106 cells |
~ 5 x 106 cells |
~ 1 x 107 cells |
Typical DNA Yield [μg] |
< 120 ng |
< 12 μg |
< 60 μg |
< 120 μg |
Animal tissue |
Amount of Starting Sample |
~ 10 mg |
~ 25 mg |
~ 100 mg |
~ 250 mg |
Typical DNA Yield [μg] |
< 5 μg |
< 10 μg |
< 40 μg |
< 120 μg |
Bacteria (Gram (-), (+)) |
Amount of Starting Sample |
- |
~ 1 x 109 cells |
~ 5 x 109 cells |
~ 1 x 1010 cells |
Typical DNA Yield [μg] |
- |
< 15 μg |
< 80 μg |
< 150 μg |
DNA purity |
A260/280 > 1.8 |
Turnaround Time |
> 5 min |
> 5 min |
> 10 min |
> 15 min |
Various kinds of forensic sample |
Amount of Starting Sample |
Dried body fluid spot or fingerprint (FTA card, paper, cloth, etc) |
~ 7 mm or ~ 2 cm2 |
Hair |
1 cm |
Bone and teeth |
~ 100 mg |
Chewing gum |
~ 30 mg |
Cigarette butts |
~ 2 cm2 |
Buccal swab |
Single piece of swab |
Urine |
~ 15 mL |
Saliva |
1-100 μL |
Typical DNA Yield [μg] |
~ 5 μg |
DNA purity |
A260/280 > 1.8 |
Turnaround Time |
> 10 min |
* 시료의 종류에 따라 측정 값의 차이가 있을 수 있습니다.
▶ 구성품
Components |
Volume |
Quantity* |
TL Buffer |
15 mL |
1 ea |
GB Buffer |
15 mL |
1 ea |
WM1 Buffer |
30 mL |
1 ea |
WB2 Buffer |
10 mL |
1 ea |
WE Buffer |
40 mL |
1 ea |
EA Buffer |
25 mL |
1 ea |
Proteinase K powder, lyophilized |
25 mg |
1 ea |
RNase A powder, lyophilized |
24 mg |
1 ea |
Poly(A), lyophilized |
1 mg |
1 ea |
Magnetic Nanobead |
6 mL |
1 ea |
1.5 mL Tube |
50 ea |
1 pack |
One Page Protocol |
- |
1 ea |
* Mini-50 rxn, Midi-7 rxn, Maxi-3 rxn
▶ 실험 자료

|
M: 1 kb ladder (Cat. No. D-1040, Bioneer) BIONEER: EcoQprep™ Genomic DNA Kit A: Competitor A Kit
|
Sample |
EcoQprep™ |
Competitor A |
Yield (μg) |
Purity (A260/280) |
Yield (μg) |
Purity (A260/280) |
Whole blood (Human blood) |
6.45 μg |
1.85 |
5.53 μg |
1.85 |
Animal tissue (porcrine skeletal muscle tissue) |
5.48 μg |
1.86 |
5.60 μg |
1.85 |
HeLa Cell |
24.1 μg |
1.92 |
23.3 μg |
2.00 |
E. coli |
14.7 μg |
1.83 |
15.5 μg |
2.05 |
Figure 1. Comparison of Genomic DNA Extraction from Various Samples using EcoQprep™ Genomic DNA Kit and Competitor’s kit (column type).
(a) Whole blood: DNA extracted from 200 µL
(b) Animal tissue: DNA extracted from 15 mg of porcine skeletal muscle tissue
(c) HeLa cell: DNA extracted from 1x10⁶ cells/rxn
(d) Escherichia coli : DNA extracted from 1x10⁹ cells/rxn (DH5α)
 |
M: 1 kb ladder (Cat. No. D-1040, Bioneer) Mini, Midi: EcoQprep™ Genomic DNA Kit
|
Scale |
Mini |
Midi |
Sample |
Starting amount |
Yield (μg) |
Purity (A260/280) |
Starting amount |
Yield (μg) |
Purity (A260/280) |
Whole blood (Human blood) |
100 μL |
3 μg |
1.80 |
1 mL |
30.85 μg |
1.82 |
Animal tissue (porcrine skeletal muscle tissue) |
20 mg |
5.48 μg |
1.81 |
100 mg |
23.9 μg |
1.81 |
HeLa Cell |
1 x 106 cells |
13.6 μg |
1.83 |
5 x 106 cells |
75.3 μg |
1.83 |
E. coli |
1 x 109 cells |
14.7 μg |
1.83 |
5 x 109 cells |
83.9 μg |
1.80 |
Figure 2. Genomic DNA Extraction from Various Samples using EcoQprep™ Genomic DNA Kit at Mini and Midi scales.

Figure 3. Comparison of real-time qPCR data of genomic DNA from HeLa cell (1 x 101 - 1 x 105) purified with EcoQprep™ Genomic DNA Kit and competitor’s kit (single column type).
(a) Amplication curve of GAPDH from HeLa cell (1 x 101 - 1 x 105) genomic DNA purified with Bioneer EcoQprep™ Genomic DNA Kit.
The premix used was Accupower® Plus DualStar™ qPCR PreMix (K-6600).
(b) Comparison of standard curves from qPCR results using genomic DNA purified with Bioneer EcoQprep™ DNA Kit and competitor A kit.

Sample |
Whole blood |
Saliva |
Fingerprint |
EcoQprep™
|
28.54 |
28.07 |
36.33 |
28.18 |
28.43 |
36.29 |
Competitor A
|
28.64 |
28.1 |
36.45 |
29.08 |
28.59 |
36.45 |
Figure 4. Genomic DNA Extraction from Paper Using EcoQprep™ Genomic DNA Kit Compared with a Competitor’s kit (column type).
After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Blood: DNA extracted from a 7 mm diameter punched sample of dried human whole blood (25 µL) on Whatman FTA card.
(b) Saliva: DNA extracted from a 7 mm diameter punched sample of dried human saliva (10 µL) on paper.
(c) Fingerprint: DNA extracted from a 7 mm diameter punched sample of fingerprint traces on paper.

Sample |
Whole blood |
Saliva |
EcoQprep™
|
29.15 |
30.30 |
29.14 |
30.60 |
Competitor A
|
29.69 |
32.05 |
29.89 |
32.14 |
Figure 5. Genomic DNA Extraction from Fabric Using EcoQprep™ Genomic DNA Kit Compared with a Competitor’s kit (column type).
After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Blood: DNA extracted from a 7 mm diameter punched samples of human blood (10 µL) stains on fabric.
(b) Saliva: DNA extracted from a 7 mm diameter punched samples of human saliva (10 µL) stains on fabric.

Scale |
Bone |
Nail Clipping |
Hair (with root) |
Hair (without root |
Cotton Swab |
Chewing Gum |
EcoQprep™ |
29.63 |
28.00 |
29.99 |
33.39 |
26.41 |
32.10 |
29.48 |
27.98 |
30.04 |
33.08 |
26.29 |
32.25 |
Competitor A |
29.63 |
30.04 |
30.7 |
34.66 |
26.87 |
31.21 |
28.75 |
29.46 |
30.4 |
34.22 |
26.45 |
31.10 |
Figure 6. Genomic DNA Extraction Using EcoQprep™ Genomic DNA Kit Compared with a Competitor’s kit (column type).
After DNA extraction, real-time PCR is performed using the AccuPower® Plus DualStar™ qPCR PreMix with GAPDH as the target.
(a) Bone: DNA extraction from porcine bone 100 mg.
(b) Nail Clipping: DNA extraction from 15 mg of nail clipping.
(c) Hair: DNA extraction from 1 strand of hair with or without root.
(d) Cotton swab: DNA extraction from cotton swab with human saliva.
(e) Chewing Gum: DNA extraction from chewing gum with human saliva.