▶ 제품 규격/사양
| Product specifications of scale |
| Scale |
Mini |
Midi |
| Expected RNA yield |
~ 100 μg |
~ 500 μg |
| Minimum elution volume |
50 µL |
500 µL |
| Preparation time |
~ 5 min |
~ 10 min |
| Product details by sample type |
| Sample |
Starting amount |
RNA yield |
Expected purity* |
| Cultured cells |
104 - 108 cells |
15 - 20 μg |
A260/280 > 2.0
A260/230 > 1.7
|
| Liver |
25 - 50 mg |
10 - 60 μg |
| Spleen |
100 mg |
30 - 60 μg |
| Plant tissue |
100 mg |
70 - 80 μg |
*시료의 종류에 따라 측정 값의 차가 있을 수 있습니다.
▶ 구성품
| Components |
Volume |
Quantity* |
| Magnetic Nanobead-RNA (50 mg/mL) |
6 mL |
1 ea |
| RD Buffer |
30 mL |
1 ea |
| RWMB1 Buffer |
25 mL |
1 ea |
| RWB2 Buffer |
15 mL |
1 ea |
| WE Buffer |
40 mL |
1 ea |
| ER Buffer |
25 mL |
1 ea |
| 1.5 mL Tube |
50 ea |
1 pack |
| One Page Protocol |
- |
1 ea |
*mini-50 rxn, midi-5 rxn
▶ 실험 자료
Comparison of RNA extracted with EcoQprep™ Universal RNA Kit and Competitor A Kit (column type). Total RNA was extracted using EcoQprep™ Universal RNA Kit and Competitor A kit (column type), and analyzed by agarose gel electrophoresis. Concentration and purity were measured using a Nanodrop.
 |
 |
 |
| Product |
Conc.
(ng/μL) |
Purity |
| A260/A280 |
A260/A230 |
| EcoQprep™ |
249.9 |
2.08 |
2.19 |
| 282.8 |
2.05 |
2.24 |
| Competitor A |
228.6 |
2.07 |
2.17 |
| 205.2 |
2.08 |
1.54 |
|
Figure 1. Cultured cell RNA extraction using EcoQprep™ Universal RNA Kit.
RNA extraction from 1 x 106 cells of HeLa cell.
DNase was not treated.
|
|
| Product |
Conc.
(ng/μL) |
Purity |
| A260/A280 |
A260/A230 |
| EcoQprep™ |
249.9 |
2.08 |
2.19 |
| 282.8 |
2.05 |
2.24 |
| Competitor A |
228.6 |
2.07 |
2.17 |
| 205.2 |
2.08 |
1.54 |
|
Figure 2. Plant RNA extraction using EcoQprep™ Universal RNA Kit.
RNA extraction from 100 mg of Brassica oleracea var. italica. DNase was not treated.
|
|
| Product |
Conc.
(ng/μL) |
Purity |
| A260/A280 |
A260/A230 |
| EcoQprep™ |
249.9 |
2.08 |
2.19 |
| 282.8 |
2.05 |
2.24 |
| Competitor A |
228.6 |
2.07 |
2.17 |
| 205.2 |
2.08 |
1.54 |
|
Figure 3. Animal tissue RNA extraction using EcoQprep™ Universal RNA Kit.
RNA extraction 20 mg of Mus musculus liver tissue. DNase was not treated.
|
|
 |
 |
 |
| Prep kit |
No. |
Conc. (ng/μL) |
Purity |
| A260/A280 |
A260/A230 |
| EcoQprep™ |
1 |
161.4 |
2.03 |
2.03 |
| 2 |
224.7 |
2.03 |
1.00 |
| 3 |
244.2 |
2.05 |
1.99 |
| 4 |
262.5 |
2.05 |
2.11 |
| 5 |
254.5 |
2.04 |
2.07 |
| 6 |
245.3 |
2.06 |
1.91 |
| 7 |
188.2 |
2.04 |
1.86 |
| 8 |
250.8 |
2.03 |
1.98 |
|
Figure 4. Cultured cell RNA extraction using EcoQprep™ Universal RNA Kit on ExiPrep™ 96 Lite.
RNA extraction from 1 x 106 cells of HeLa. DNase was not treated. (average of concentration is 228.9 ng/μL, average A260/A280 is 2.04).
|
|
| Prep kit |
No. |
Conc. (ng/μL) |
Purity |
| A260/A280 |
A260/A230 |
| EcoQprep™ |
1 |
704.2 |
2.16 |
2.40 |
| 2 |
586.8 |
2.20 |
2.39 |
| 3 |
823.1 |
2.17 |
2.43 |
| 4 |
629.7 |
2.19 |
2.33 |
| 5 |
700.0 |
2.17 |
2.34 |
| 6 |
707.0 |
2.18 |
2.30 |
| 7 |
609.4 |
2.20 |
2.22 |
| 8 |
516.2 |
2.18 |
2.35 |
|
Figure 5. Plant tissue RNA extraction using EcoQprep™ Universal RNA Kit on ExiPrep™ 96 Lite.
RNA extraction from ~ 100 mg of Brassica oleracea. DNase was not treated.
(average of concentration is 659.5 ng/μL, average A260/A280 is 2.18).
|
|
| Prep kit |
No. |
Conc. (ng/μL) |
Purity |
| A260/A280 |
A260/A230 |
| EcoQprep™ |
1 |
774.6 |
2.01 |
2.33 |
| 2 |
690.2 |
2.03 |
2.41 |
| 3 |
694.4 |
2.07 |
1.91 |
| 4 |
706.9 |
2.02 |
2.39 |
| 5 |
759.8 |
2.03 |
2.37 |
| 6 |
670.4 |
2.02 |
2.40 |
| 7 |
802.5 |
2.03 |
2.37 |
| 8 |
884.3 |
2.06 |
2.40 |
|
Figure 6. Animal tissue RNA extraction using EcoQprep™ Universal RNA Kit on ExiPrep™ 96 Lite.
RNA extraction from ~ 20 mg of mouse kidney. DNase was not treated. (average of concentration is 747.9 ng/μL, average A260/A280 is 2.03).
|
|
