AccuTarget™ GAPDH Positive Control siRNA

AccuTarget™ Positive control siRNA는 target gene에 대하여 높은 효율의 knockdown효과를 나타냅니다.
널리 사용되는Housekeeping gene인 GAPDH에 대한 효율적인 siRNA를 제공하며 reporter system으로 GFP와 Luciferase에 대한 Positive Control siRNA도 제공합니다.

₩68,000
In stock
SKU
SP-1001-CFG

Features and Benefits

Excellent Performance:
90% 이상의 knockdown 효율을 갖는 positive control siRNA

Monitoring of Transfection Rate:
형광물질로 labeling되어 편리하게 효능 검증이 가능한 negative control sets

Competitive Pricing:
연구비용 대비 극대화된 효율


Positive and Negative Control siRNA

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A) Northern Blotting B) qRT-PCR

Figure 1. Effects of Human GAPDH Positive Control siRNA.
HeLa cells were transfected separately with AccuTarget™ Human GAPDH Positive Control and Negative Control siRNA using Lipofectamine 2000 (Invitrogen) at a final concentration of 100 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to northern blot and real-time PCR analysis. As can be seen from Fig.1-B, about 3% GAPDH mRNA remained.

GFP Control siRNA

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Figure 2. HeLa cells in a 24-well plate were cotransfected with 200 ng of CMV-GFP plasmid and 10 nM of GFP siRNA using Lipofectamine 2000 transfection reagent. Next day, the expression of GFP was observed by using a Nikon Eclipse TS100 epifluorescence microscope. In contrast to bright green fluorescence of GFP protein in NC-siRNA-transfected cells, no fluorescence was detected from GFP-siRNA-transfected cells, indicating efficient knockdown of GFP by using our positive control GFP-siRNA.

GFP Control siRNA

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Figure 3. HeLa cells in a 6-well plate were cotransfected with 400 ng of CMV-luc plasmid and 10 nM of luciferase siRNA using lipofectamine 2000 transfection reagent. Next day, cells were harvested and assayed for luciferase activity. As shown in Fig. 3, cotransfection with our positive control luciferase siRNA led to efficient knockdown of luciferase activity (85% - 95% knockdown compared to luciferase activity of NC-siRNA-transfected cells).

Mouse Positive Control siRNA

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Figure 4. Effects of Mouse Lamin A/C Positive Control siRNA.
Hepa1-6 cells were transfected separately with AccuTarget™ Mouse Lamin A/C Positive Control and Negative Control siRNA using Lipofectamine RNAimax (Invitrogen) at a final concentration of 1 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to real-time PCR analysis.


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Figure 5. Effects of Mouse Ppib (Cyclophilin B) Positive Control siRNA.
Hepa1-6 & NIH 3T3 cells were transfected separately with AccuTarget™ Mouse Ppib (Cyclophilin B) Positive Control and Negative Control siRNA using Lipofectamine RNAimax (Invitrogen) at a final concentration of 5 & 1 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to real-time PCR analysis. 

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