AccuTarget™ GAPDH Positive Control siRNA

AccuTarget™ Positive control siRNA는 target gene에 대하여 높은 효율의 knockdown효과를 나타냅니다.
널리 사용되는Housekeeping gene인 GAPDH에 대한 효율적인 siRNA를 제공하며 reporter system으로 GFP와 Luciferase에 대한 Positive Control siRNA도 제공합니다.

₩68,000
카탈로그 번호
SP-1001-CFG

개요

  • AccuTarget ™ PC siRNA (Human GAPDH, GFP, Luciferase) : 고효율의 타겟 유전자 KD 효과를 검증하였습니다.
  • AccuTarget ™ NC siRNA (Human, Mouse, Rat 공용) : 타겟 유전자와 homology가 낮은 서열의 제품입니다.
  • Fluorescently labeled siRNA에 의해 간단하게 siRNA delivery 모니터링 할 수 있습니다.
  • AccuTarget ™ Control siRNA Sets (PC+ NC) : 간편하고 저렴한 control set를 함께 구매할 수 있습니다.
  • 다양한 scale (5, 10, 20 nmole) 및 purification (BioRP, HPLC) 선택할 수 있습니다.

특장점

  • 높은 효율

    90% 이상의 knockdown 효율을 갖는 positive control siRNA 입니다.

  • 편리한 모니터링

    형광물질로 labeling되어 편리하게 Transfection Rate에 대해 효능 검증이 가능한 negative control sets 입니다.

  • 합리적인 가격

    연구비용 대비 극대화된 효율을 확인할 수 있습니다.

실험 자료

• Positive and Negative Control siRNA

control figure1   control figure2

A) Northern Blotting B) qRT-PCR

Figure 1. Effects of Human GAPDH Positive Control siRNA.
HeLa cells were transfected separately with AccuTarget ™ Human GAPDH Positive Control and Negative Control siRNA using Lipofectamine 2000 (Invitrogen) at a final concentration of 100 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to northern blot and real-time PCR analysis. As can be seen from Fig.1-B, about 3% GAPDH mRNA remained.

• GFP Control siRNA

control figure3

Figure 2. HeLa cells in a 24-well plate were cotransfected with 200 ng of CMV-GFP plasmid and 10 nM of GFP siRNA using Lipofectamine 2000 transfection reagent. Next day, the expression of GFP was observed by using a Nikon Eclipse TS100 epifluorescence microscope. In contrast to bright green fluorescence of GFP protein in NC-siRNA-transfected cells, no fluorescence was detected from GFP-siRNA-transfected cells, indicating efficient knockdown of GFP by using our positive control GFP-siRNA.

• Luciferase Control siRNA

control figure4

Figure 3. HeLa cells in a 6-well plate were cotransfected with 400 ng of CMV-luc plasmid and 10 nM of luciferase siRNA using lipofectamine 2000 transfection reagent. Next day, cells were harvested and assayed for luciferase activity. As shown in Fig. 3, cotransfection with our positive control luciferase siRNA led to efficient knockdown of luciferase activity (85% - 95% knockdown compared to luciferase activity of NC-siRNA-transfected cells).

• Mouse Positive Control siRNA

control figure5

Figure 4. Effects of Mouse Lamin A/C Positive Control siRNA.
Hepa1-6 cells were transfected separately with AccuTarget ™ Mouse Lamin A/C Positive Control and Negative Control siRNA using Lipofectamine RNAimax (Invitrogen) at a final concentration of 1 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to real-time PCR analysis.


control figure6

Figure 5. Effects of Mouse Ppib (Cyclophilin B) Positive Control siRNA.
Hepa1-6 & NIH 3T3 cells were transfected separately with AccuTarget ™ Mouse Ppib (Cyclophilin B) Positive Control and Negative Control siRNA using Lipofectamine RNAimax (Invitrogen) at a final concentration of 5 & 1 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to real-time PCR analysis. 

Manual

Manual_AccuTarget ™ Genome-wide Predesigned siRNA library

Manual_Protocol for siRNA Annealing

Manual_AccuTarget ™ Real-Time PCR Primer Library


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AccuTarget ™ Positive Control siRNAs

카탈로그 번호 제품명 정제 공급량 가격
SP-1001 AccuTarget ™ GAPDH Positive Control siRNA BioRP 5 nmole 68,000원
SP-1002 AccuTarget ™ GAPDH Positive Control siRNA BioRP 10 nmole 96,000원
SP-1003 AccuTarget ™ GAPDH Positive Control siRNA BioRP 20 nmole 116,000원
SP-1011 AccuTarget ™ GAPDH Positive Control siRNA HPLC 5 nmole 94,000원
SP-1012 AccuTarget ™ GAPDH Positive Control siRNA HPLC 10 nmole 132,000원
SP-1013 AccuTarget ™ GAPDH Positive Control siRNA HPLC 20 nmole 192,000원
SP-2001 AccuTarget ™ GFP Positive Control siRNA BioRP 5 nmole 68,000원
SP-2002 AccuTarget ™ GFP Positive Control siRNA BioRP 10 nmole 96,000원
SP-2003 AccuTarget ™ GFP Positive Control siRNA BioRP 20 nmole 116,000원
SP-2011 AccuTarget ™ GFP Positive Control siRNA HPLC 5 nmole 94,000원
SP-2012 AccuTarget ™ GFP Positive Control siRNA HPLC 10 nmole 132,000원
SP-2013 AccuTarget ™ GFP Positive Control siRNA HPLC 20 nmole 192,000원
SP-3001 AccuTarget ™ Luciferase Positive Control siRNA BioRP 5 nmole 68,000원
SP-3002 AccuTarget ™ Luciferase Positive Control siRNA BioRP 10 nmole 96,000원
SP-3003 AccuTarget ™ Luciferase Positive Control siRNA BioRP 20 nmole 116,000원
SP-3011 AccuTarget ™ Luciferase Positive Control siRNA HPLC 5 nmole 94,000원
SP-3012 AccuTarget ™ Luciferase Positive Control siRNA HPLC 10 nmole 132,000원
SP-3013 AccuTarget ™ Luciferase Positive Control siRNA HPLC 20 nmole 192,000원
SP-4001 AccuTarget ™ Mouse Lamin A/C Positive Control BioRP 5nmole 68,000원
SP-4002 AccuTarget ™ Mouse Lamin A/C Positive Control BioRP 10nmole 96,000원
SP-4003 AccuTarget ™ Mouse Lamin A/C Positive Control BioRP 20nmole 116,000원
SP-4011 AccuTarget ™ Mouse Lamin A/C Positive Control HPLC 5nmole 94,000원
SP-4012 AccuTarget ™ Mouse Lamin A/C Positive Control HPLC 10nmole 132,000원
SP-4013 AccuTarget ™ Mouse Lamin A/C Positive Control HPLC 20nmole 192,000원
SP-5001 AccuTarget ™ Mouse cyclophilin B c Positive Control BioRP 5nmole 68,000원
SP-5002 AccuTarget ™ Mouse cyclophilin B c Positive Control BioRP 10nmole 96,000원
SP-5003 AccuTarget ™ Mouse cyclophilin B c Positive Control BioRP 20nmole 116,000원
SP-5011 AccuTarget ™ Mouse cyclophilin B c Positive Control HPLC 5nmole 94,000원
SP-5012 AccuTarget ™ Mouse cyclophilin B c Positive Control HPLC 10nmole 132,000원
SP-5013 AccuTarget ™ Mouse cyclophilin B c Positive Control HPLC 20nmole 192,000원

AccuTarget ™ Negative Control siRNAs

카탈로그 번호 제품명 정제 공급량 가격
SN-1001 AccuTarget ™ Negative Contol siRNA BioRP 5 nmole 68,000원
SN-1002 AccuTarget ™ Negative Contol siRNA BioRP 10 nmole 96,000원
SN-1003 AccuTarget ™ Negative Contol siRNA BioRP 20 nmole 116,000원
SN-1011 AccuTarget ™ Negative Contol siRNA HPLC 5 nmole 94,000원
SN-1012 AccuTarget ™ Negative Contol siRNA HPLC 10 nmole 132,000원
SN-1013 AccuTarget ™ Negative Contol siRNA HPLC 20 nmole 192,000원
SN-1021 AccuTarget ™ Fluorescein-labeled Negative Control siRNA HPLC 5 nmole 134,000원
SN-1022 AccuTarget ™ Fluorescein-labeled Negative Control siRNA HPLC 10 nmole 188,000원
SN-1023 AccuTarget ™ Fluorescein-labeled Negative Control siRNA HPLC 20 nmole 264,000원

AccuTarget ™ Control siRNA Sets

카탈로그 번호 제품명 정제 공급량 가격
SS-1001 AccuTarget ™ GAPDH Control siRNA Set BioRP 5 nmole positive control + 2 nmole negative control 81,000원
SS-1002 AccuTarget ™ GFP Control siRNA Set BioRP 5 nmole positive control + 2 nmole negative control 81,000원
SS-1003 AccuTarget ™ Luciferase Control siRNA Set BioRP 5 nmole positive control + 2 nmole negative control 81,000원
SS-1011 AccuTarget ™ GAPDH Control siRNA Set HPLC 5 nmole positive control + 2 nmole negative control 112,000원
SS-1012 AccuTarget ™ GFP Control siRNA Set HPLC 5 nmole positive control + 2 nmole negative control 112,000원
SS-1013 AccuTarget ™ Luciferase Control siRNA Set HPLC 5 nmole positive control + 2 nmole negative control 112,000원
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