AccuFect™ Transfection Reagent

▶ 제품 규격/사양

▶ 실험 자료

1. Transfection Reagent 종류에 따른 GFP DNA의 발현 효율 비교
   
   
   Figure 1. Comparison of expression level of GFP DNA using three types of transfection reagents from Competitor I, Competitor S, and BIONEER.
   GFP DNA was transfected into HuH-7 cell using each transfection reagent, and the expression level was observed after 48 hours. BIONEER’s AccuFect™ Transfection Reagent showed the greatest expression level. Expressed ß-actin shows that the same amount of protein is used for each experiment.
   Lane 1: Negative control (No GFP DNA)
   Lane 2: Competitor I’s Transfection Reagent
   Lane 3: Competitor S’s Transfection Reagent
   Lane 4: BIONEER’s AccuFect™ Transfection Reagent
   
   
   
2. AccuFect™ Transfection Reagent를 이용한 293T 세포주에서의 RFP DNA 발현
   
   
   Figure 2. (left) Fluorescent image and (right) fluorescent + bright field image showing the expression level of RFP DNA.
   RFP DNA was transfected into 293T cells using AccuFect™ Transfection Reagent from BIONEER and the transfection efficiency was measured to be around 95%.
   
   
   
3. AccuFect™ Transfection Reagent의 293T와 BHK-21 세포주에서의 GFP DNA 발현
   
   
   

   Figure 3. BIONEER’s AccuFect™ Transfection Reagent showed higher expression level of GFP DNA in both 293T cells and BHK-21 cells than that of the Competitor’s.
   Figure 3a-d). (top) Fluorescent image and (bottom) fluorescent and bright-field image showing the expression level of GFP DNA in:
       a)   293T cells using Competitor’s transfection reagent.
       b)   293T cells using BIONEER’s AccuFect™ Transfection Reagent.
       c)   BHK-21 cells using Competitor’s transfection reagent.
       d)   BHK-21 cells using BIONEER’s AccuFect™ Transfection Reagent.
   
   

4. AccuFect™ Transfection Reagent 이용한 siRNA HPRT1 발현 비교
   
   
   

   Figure 4. Comparison of HPRT1 expression of siRNA using transfection reagents from Competitor and BIONEER.
   BIONEER’s AccuFect™ Transfection Reagent shows a significantly better suppression of gene expression by siRNA.
   
   
   

5. AccuFect™ Transfection Reagent 사용 가능한 세포주 종류
   
   

   Cell Line	Efficiency (% GFP)	Best record
   293T	>90%	98%
   BHK-21	>80%	96%
   HuH-7	>75%	95%
   HepG2	>75%	92%
   Vero	>40%	82%
   HMEC-1	>40%	65%
   U118 MG	>25%	43%
   SKNSH	>25%	58%
   CHO-K1	>70%	90%
   MDCK	>65%	79%
   Other cell lines which can be transfected
   THP-1	K562	C6/36
   A549	HT-29	HeLa
   HUVEC	SP2/0	P3X63Ag8
   PBMCs	SF9	S2

   
   Table 1. AccuFect™ Transfection Reagent can be used to transfect many different cell lines.