AccuTarget™ Mouse cyclophilin B c Positive Control

AccuTarget™ Positive control siRNA는 target gene에 대하여 높은 효율의 knockdown효과를 나타냅니다.
널리 사용되는Housekeeping gene인 GAPDH에 대한 효율적인 siRNA를 제공하며 reporter system으로 GFP와 Luciferase에 대한 Positive Control siRNA도 제공합니다.

₩68,000
카탈로그 번호
SP-5001-CFG

Features and Benefits

Excellent Performance:
90% 이상의 knockdown 효율을 갖는 positive control siRNA

Monitoring of Transfection Rate:
형광물질로 labeling되어 편리하게 효능 검증이 가능한 negative control sets

Competitive Pricing:
연구비용 대비 극대화된 효율


Positive and Negative Control siRNA

control figure1   control figure2

A) Northern Blotting B) qRT-PCR

Figure 1. Effects of Human GAPDH Positive Control siRNA.
HeLa cells were transfected separately with AccuTarget™ Human GAPDH Positive Control and Negative Control siRNA using Lipofectamine 2000 (Invitrogen) at a final concentration of 100 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to northern blot and real-time PCR analysis. As can be seen from Fig.1-B, about 3% GAPDH mRNA remained.

GFP Control siRNA

control figure3

Figure 2. HeLa cells in a 24-well plate were cotransfected with 200 ng of CMV-GFP plasmid and 10 nM of GFP siRNA using Lipofectamine 2000 transfection reagent. Next day, the expression of GFP was observed by using a Nikon Eclipse TS100 epifluorescence microscope. In contrast to bright green fluorescence of GFP protein in NC-siRNA-transfected cells, no fluorescence was detected from GFP-siRNA-transfected cells, indicating efficient knockdown of GFP by using our positive control GFP-siRNA.

GFP Control siRNA

control figure4

Figure 3. HeLa cells in a 6-well plate were cotransfected with 400 ng of CMV-luc plasmid and 10 nM of luciferase siRNA using lipofectamine 2000 transfection reagent. Next day, cells were harvested and assayed for luciferase activity. As shown in Fig. 3, cotransfection with our positive control luciferase siRNA led to efficient knockdown of luciferase activity (85% - 95% knockdown compared to luciferase activity of NC-siRNA-transfected cells).

Mouse Positive Control siRNA

control figure5

Figure 4. Effects of Mouse Lamin A/C Positive Control siRNA.
Hepa1-6 cells were transfected separately with AccuTarget™ Mouse Lamin A/C Positive Control and Negative Control siRNA using Lipofectamine RNAimax (Invitrogen) at a final concentration of 1 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to real-time PCR analysis.


control figure6

Figure 5. Effects of Mouse Ppib (Cyclophilin B) Positive Control siRNA.
Hepa1-6 & NIH 3T3 cells were transfected separately with AccuTarget™ Mouse Ppib (Cyclophilin B) Positive Control and Negative Control siRNA using Lipofectamine RNAimax (Invitrogen) at a final concentration of 5 & 1 nM. Total cellular RNA was isolated from transfected cells 24 hours after transfection and subjected to real-time PCR analysis. 

AccuTarget™ Positive Control siRNAs

Cat. No. Description Purification Guaranteed Yield Price
SP-1001 AccuTarget™ GAPDH Positive Control siRNA BioRP 5 nmole 68,000원
SP-1002 AccuTarget™ GAPDH Positive Control siRNA BioRP 10 nmole 96,000원
SP-1003 AccuTarget™ GAPDH Positive Control siRNA BioRP 20 nmole 116,000원
SP-1011 AccuTarget™ GAPDH Positive Control siRNA HPLC 5 nmole 94,000원
SP-1012 AccuTarget™ GAPDH Positive Control siRNA HPLC 10 nmole 132,000원
SP-1013 AccuTarget™ GAPDH Positive Control siRNA HPLC 20 nmole 192,000원
SP-2001 AccuTarget™ GFP Positive Control siRNA BioRP 5 nmole 68,000원
SP-2002 AccuTarget™ GFP Positive Control siRNA BioRP 10 nmole 96,000원
SP-2003 AccuTarget™ GFP Positive Control siRNA BioRP 20 nmole 116,000원
SP-2011 AccuTarget™ GFP Positive Control siRNA HPLC 5 nmole 94,000원
SP-2012 AccuTarget™ GFP Positive Control siRNA HPLC 10 nmole 132,000원
SP-2013 AccuTarget™ GFP Positive Control siRNA HPLC 20 nmole 192,000원
SP-3001 AccuTarget™ Luciferase Positive Control siRNA BioRP 5 nmole 68,000원
SP-3002 AccuTarget™ Luciferase Positive Control siRNA BioRP 10 nmole 96,000원
SP-3003 AccuTarget™ Luciferase Positive Control siRNA BioRP 20 nmole 116,000원
SP-3011 AccuTarget™ Luciferase Positive Control siRNA HPLC 5 nmole 94,000원
SP-3012 AccuTarget™ Luciferase Positive Control siRNA HPLC 10 nmole 132,000원
SP-3013 AccuTarget™ Luciferase Positive Control siRNA HPLC 20 nmole 192,000원

SP-4001
AccuTarget™ Mouse Lamin A/C Positive Control BioRP 5nmole 68,000원

SP-4002
AccuTarget™ Mouse Lamin A/C Positive Control BioRP 10nmole 96,000원

SP-4003
AccuTarget™ Mouse Lamin A/C Positive Control BioRP 20nmole 116,000원

SP-4011
AccuTarget™ Mouse Lamin A/C Positive Control HPLC 5nmole 94,000원

SP-4012
AccuTarget™ Mouse Lamin A/C Positive Control HPLC 10nmole 132,000원

SP-4013
AccuTarget™ Mouse Lamin A/C Positive Control HPLC 20nmole 192,000원

SP-5001
AccuTarget™ Mouse cyclophilin B c Positive Control BioRP 5nmole 68,000원

SP-5002
AccuTarget™ Mouse cyclophilin B c Positive Control BioRP 10nmole 96,000원

SP-5003
AccuTarget™ Mouse cyclophilin B c Positive Control BioRP 20nmole 116,000원

SP-5011
AccuTarget™ Mouse cyclophilin B c Positive Control HPLC 5nmole 94,000원

SP-5012
AccuTarget™ Mouse cyclophilin B c Positive Control HPLC 10nmole 132,000원

SP-5013
AccuTarget™ Mouse cyclophilin B c Positive Control HPLC 20nmole 192,000원


AccuTarget™ Negative Control siRNAs

Cat. No. Description Purification Guaranteed Yield Price
SN-1001 AccuTarget™ Negative Contol siRNA BioRP 5 nmole 68,000원
SN-1002 AccuTarget™ Negative Contol siRNA BioRP 10 nmole 96,000원
SN-1003 AccuTarget™ Negative Contol siRNA BioRP 20 nmole 116,000원
SN-1011 AccuTarget™ Negative Contol siRNA HPLC 5 nmole 94,000원
SN-1012 AccuTarget™ Negative Contol siRNA HPLC 10 nmole 132,000원
SN-1013 AccuTarget™ Negative Contol siRNA HPLC 20 nmole 192,000원
SN-1021 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 5 nmole 134,000원
SN-1022 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 10 nmole 188,000원
SN-1023 AccuTarget™ Fluorescein-labeled Negative Control siRNA HPLC 20 nmole 264,000원


AccuTarget™ Control siRNA Sets

Cat. No. Description Purification Guaranteed Yield Price
SS-1001 AccuTarget™ GAPDH Control siRNA Set BioRP 5 nmole positive control + 2 nmole negative control 81,000원
SS-1002 AccuTarget™ GFP Control siRNA Set BioRP 5 nmole positive control + 2 nmole negative control 81,000원
SS-1003 AccuTarget™ Luciferase Control siRNA Set, BioRP 5 nmole positive control + 2 nmole negative control 81,000원
SS-1011 AccuTarget™ GAPDH Control siRNA Set HPLC 5 nmole positive control + 2 nmole negative control 112,000원
SS-1012 AccuTarget™ GFP Control siRNA Set HPLC 5 nmole positive control + 2 nmole negative control 112,000원
SS-1013 AccuTarget™ Luciferase Control siRNA Set HPLC 5 nmole positive control + 2 nmole negative control 112,000원
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