AccuPower® PCR PreMix (with UDG)

AccuPower® PCR PreMix (with UDG)는 임상 진단이나 실험 시, PCR 반응에 있어 심각한 문제를 야기할 수 있는 crossover contamination이나 carryover contamination을 최소화할 수 있는 제품입니다. PCR은 민감하고 신속한 검사법 중 하나이지만, 이전에 증폭된 PCR 산물의 오염에 의해 위양성의 문제가 흔히 발생할 수 있습니다. 본 제품은 uracil DNA glycosylase system을 적용하여 이러한 문제점을 극복했습니다.

₩83,000
In stock
SKU
K-2012-1-CFG

Features and Benefits

Prevention of Carryover Contamination:
Uracil DNA glycosylase system 적용으로 반복적인 PCR 실험으로 인해 공기 중에 남아있던 산물이 유입되어 나타나는 carryover contamination을 방지합니다. Uracil DNA Glycosylase (UDG)는 DNA 가닥 내 uracil과 deoxyribose간의 N-glycosylic bond를 가수분해 함으로써 uracil이 삽입된 template을 제거합니다. UDG는 PCR cycling 전 37℃에서 2분 반응을 통해 활성화되어, uracil이 들어간 DNA를 제거하므로 공기 중 남아있던 산물의 유입에 의한 carryover 오염을 방지할 수 있으며, PCR cycling 중 고온에서 불활성화됩니다.

accupower

Ease-of-Use:
Uracil DNA glycosylase, dUTP, DNA polymerase, dNTPs를 포함하여 PCR 수행에 필요한 모든 반응물이 동결 건조된 premix 형태로 1회 분량씩 개별 포장되어 있습니다. 실험자는 template DNA, primer set, D.W.만 넣어 바로 PCR 반응을 수행할 수 있습니다. 또한 전기 영동 시에 필요한 tracking dye와 침강제가 포함되어 있어 별도로 sample loading buffer를 첨가할 필요가 없으므로 간편하게 사용할 수 있습니다.

Stability: 
PCR 반응 혼합물에 안정화제를 첨가하여, -20℃에서 2년까지 효소 활성이 안정적으로 유지됩니다.

Reproducibility:
ISO 9001 품질 시스템 하에서 one-batch system으로 대량 생산되어 각 batch에 대한 철저한 QC를 거친 후 균일한 품질의 제품으로 공급되기 때문에 사용자가 대량의 시료를 반복적으로 처리할 때 각 tube마다 발생하는 편차 문제를 해결하여 재현성있는 실험 결과를 제공합니다.

Specifications

Enzyme: Top DNA polymerase
5' to 3' exonuclease activity: No
3' to 5' exonuclease activity: No
Terminal transferase activity: Yes
Fragment Size: Up to 10 kb


Applications

- Conventional PCR
- Primer extension
- TA cloning
- Gene sequencing
- Molecular diagnosis


premix figure1

Figure 1. Comparison of sensitivity between AccuPower® PCR PreMix and AccuPower® PCR PreMix (with UDG)
Sensitivity test was operated using serial diluted human genomic DNA. Reaction mixture was incubated at 37°C for 2min followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C. AccuPower® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.

The amount of DNA used to test is represented below.

Lane 1; 10 ng of Human genomic DNA
Lane 2; 1 ng of Human genomic DNA
Lane 3; 100 pg of Human genomic DNA
Lane 4; 10 pg of Human genomic DNA
M; 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)


premix figure2

Figure 2.Comparison of amplification quality using PCR products (not including uracil base) between AccuPower® PCR PreMix and AccuPower® PCR PreMix (with UDG).

Amplification quality test was operated using serial diluted PCR products not including uracil base. AccuPower® PCR PreMix was also tested for negative control. Reaction mixture was incubated at 37°C for 2min, followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C. AccuPower® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP. The copy number of PCR products (copy) used to test is represented below.

Lane 1; 1011copy
Lane 2; 1010copy
Lane 3; 109copy
Lane 4; 108copy
Lane 5; 107copy
Lane 6; 106copy
Lane 7; 105copy
Lane 8; 104copy
Lane 9; 103copy
Lane 10; 102copy
Lane N; No template control
M; 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)


premix figure3

Figure 3.Efficiency of uracil DNA glycosylase using PCR products (including uracil base)
Efficiency test of uracil DNA glycosylase was operated using serial diluted PCR products including uracil base. AccuPower® PCR PreMix was also tested for negative control. Reaction mixture was incubated at 37°C for 2min, followed by 95°C for 5min, 30 cycles of 20sec at 95°C, 20sec at 55°C, 30sec at 72°C. AccuPower® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.

The copy number of PCR products used to test is represented below.

Lane 1; 1011copy
Lane 2; 1010copy
Lane 3; 109copy
Lane 4; 108copy
Lane 5; 107copy
Lane 6; 106copy
Lane 7; 105copy
Lane 8; 104copy
Lane 9; 103copy
Lane 10; 102copy
Lane N; No template control
M; 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)


Manual

• Manual_AccuPower® PCR PreMix(with UDG)


MSDS

• MSDS_AccuPower® PCR PreMixs(with UDG)


Brochure

• All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

• EN ISO 13485 - certificate














Cat. No. Product Description Price
K-2012-1 AccuPower® PCR PreMix (with UDG), 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes ₩83,000
K-2016-1 AccuPower® PCR PreMix (with UDG), 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes ₩311,000


























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