AccuPower® PCR PreMix (with UDG)

AccuPower® PCR PreMix (with UDG)는 임상 진단이나 실험 시, PCR 반응에 있어 심각한 문제를 야기할 수 있는 교차 오염을 최소화할 수 있는 제품입니다. PCR은 민감하고 신속한 검사법 중 하나이지만, 이전에 증폭된 PCR 산물의 오염에 의해 위양성의 문제가 흔히 발생할 수 있습니다. 본 제품은 uracil DNA glycosylase system을 적용하여 이러한 문제점을 극복했습니다.

₩83,000
카탈로그 번호
K-2012-1-CFG

특장점

  • 교차 오염 방지

    Uracil DNA glycosylase system 이 적용되어 교차 오염(Carryover contamination)에 의한 위양성을 최소화로 방지할 수 있습니다.

    accupower

  • 편리성

    각 PCR tube에 1회 반응씩 PCR 반응물이 건조되어 있어 누구나 손쉽게 template DNA 및 primer 만을 첨가하여 PCR 반응을 수행할 수 있습니다.

  • 안정성

    PCR 반응 혼합물에 안정화제를 첨가하여, -20℃에서 2년까지 효소 활성이 안정적으로 유지됩니다.

  • 재현성

    ISO 9001 품질 시스템 하에서 대량 생산되어 lot 간 편차를 최소화 되었으며, 반복적인 실험에도 재현성 있는 실험 결과를 제공합니다.

응용 및 적용

• Conventional PCR

• Primer extension

• TA cloning

• Gene sequencing

• Molecular diagnosis

실험 자료


premix figure1

Figure. Comparison of sensitivity between AccuPower® PCR PreMix and AccuPower® PCR PreMix (with UDG)
Sensitivity test was operated using serial diluted human genomic DNA. Reaction mixture was incubated at 37°C for 2 min followed by 95°C for 5 min, 30 cycles of 20 sec at 95°C, 20 sec at 55°C, 30 sec at 72°C. AccuPower® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.

The amount of DNA used to test is represented below.

Lane 1; 10 ng of Human genomic DNA
Lane 2; 1 ng of Human genomic DNA
Lane 3; 100 pg of Human genomic DNA
Lane 4; 10 pg of Human genomic DNA
M; 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)

제품 규격/사양

Enzyme

Top DNA polymerase

5' to 3' exonuclease activity

No

3' to 5' exonuclease activity

No

Terminal transferase activity

Yes

Fragment Size

Up to 10 kb

실험 자료

premix figure2

Figure 1.Comparison of amplification quality using PCR products (not including uracil base)
between AccuPower® PCR PreMix and AccuPower® PCR PreMix (with UDG).

Amplification quality test was operated using serial diluted PCR products not including uracil base.
AccuPower® PCR PreMix was also tested for negative control. Reaction mixture was incubated at 37°C for 2 min, followed by 95°C for 5 min, 30 cycles of 20 sec at 95°C, 20 sec at 55°C, 30 sec at 72°C.
AccuPower® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.

The copy number of PCR products (copy) used to test is represented below.

Lane 1; 1011 copy
Lane 2; 1010 copy
Lane 3; 10copy
Lane 4; 10copy
Lane 5; 10copy
Lane 6; 10copy
Lane 7; 10copy
Lane 8; 10copy
Lane 9; 10copy
Lane 10; 10copy
Lane N; No template control
M; 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)


premix figure3

Figure 2. Efficiency of uracil DNA glycosylase using PCR products (including uracil base)
Efficiency test of uracil DNA glycosylase was operated using serial diluted PCR products including uracil base.
AccuPower® PCR PreMix was also tested for negative control. Reaction mixture was incubated at 37°C for 2 min, followed by 95°C for 5 min, 30 cycles of 20 sec at 95°C, 20 sec at 55°C, 30 sec at 72°C. AccuPower® PCR PreMix (with UDG) contains dUTP besides dATP, dGTP, dCTP and dTTP.

The copy number of PCR products used to test is represented below.

Lane 1; 1011 copy
Lane 2; 1010 copy
Lane 3; 10copy
Lane 4; 10copy
Lane 5; 10copy
Lane 6; 10copy
Lane 7; 10copy
Lane 8; 10copy
Lane 9; 10copy
Lane 10; 10copy
Lane N; No template control
M; 100 bp DNA ladder (Bioneer Cat. No: D-1030-1)

Manual

Manual_AccuPower® PCR PreMix (with UDG)


MSDS

MSDS_AccuPower® PCR PreMixs (with UDG)


Brochure

All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate

카탈로그 번호 제품명 가격
K-2012-1 AccuPower® PCR PreMix (with UDG), 0.2 ml thin-wall 8-strip tubes with attached cap / 96 tubes ₩83,000
K-2016-1 AccuPower® PCR PreMix (with UDG), 0.2 ml thin-wall 8-strip tubes with attached cap / 480 tubes ₩311,000
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