AccuPower® ProFi Taq PCR PreMix

AccuPower® ProFi Taq PCR PreMix는 Bioneer 에서 개발한 ProFi Taq DNA polymerase을 사용하여 높은 정확성과 증폭효율로 긴 template DNA를 정확하게 증폭할 수 있는 제품입니다. ProFi Taq DNA polymerase와 dNTPs, reaction buffer 등 PCR 수행에 필요한 구성성분이 혼합되어 1회 분량씩 진공 건조되어 있어, template DNA 와 primers의 첨가만으로 간편하게 고성능 PCR product를 얻을 수 있습니다. 

₩87,000
카탈로그 번호
K-2631-CFG

특장점

  • 고효율, 고감도 PCR 증폭

    뛰어난 민감도와 최고의 증폭효율로 정확한 PCR product를 얻을 수 있습니다.

  • Long Range PCR

    Human DNA 20 kb 까지 증폭할 수 있습니다.

  • 사용 편의성

    PCR 수행에 필요한 성분이 진공 건조되어 있어 template와 Primer의 첨가만으로도 편리한 실험이 가능합니다.

  • 안정성

    PCR 반응 혼합액에 안정화제가 포함되어 있어 상온배송이 가능하고 solution type 제품보다 안정성이 뛰어납니다.

  • 재현성

    ISO 9001 품질 시스템 하에서 대량 생산되어 lot 간 편차가 최소화 되었으며, 반복적인 실험에도 재현성 있는 실험 결과를 제공합니다.

응용 및 적용

• Primer extension

• long-range amplification from genomic DNA

• High amplification efficiency

• Excellent performance on difficult templates

• Amplification of low-copy targets

• High yield and high sensitivity PCR

실험 자료

accupower

Figure. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reaction using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target : human Insulin receptor gene.
Lane M : 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human genomic DNA
Lane 2 : 1 ng of human genomic DNA
Lane 3 : 100 pg of human genomic DNA
Lane 4 : 10 pg of human genomic DNA

제품 규격/사양

5' to 3' exonuclease activity

Yes

3' to 5' exonuclease activity

Yes

3'- A overhang

Yes

PCR product size

~ 30 kb

실험 자료

accupower

Figure 1. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
cDNA synthesized from 10-fold serial-diluted human total RNA from 10 ng to 10 pg using AccuPower® RocketScript ™ Cycle RT PreMix.(Bioneer, Cat. No K-2201) wase used as a template for PCR amplification. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 33 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target : human GAPDH gene.
Lane M : 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human total cDNA
Lane 2 : 1 ng of human total cDNA
Lane 3 : 100 pg of human total cDNA
Lane 4 : 10 pg of human total cDNA



accupower

Figure 2. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec and 68°C for 4 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Lane M : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 2 kb fragment (human tumor protein p53 gene)
Lane 2 : 3 kb fragment (human tumor protein p53 gene)
Lane 3 : 4.5kb fragment (human DNA cross-link repair 1A gene)
Lane 4 : 8 kb fragment (human hemoglobin epsilon 1 gene



accupower

Figure 3. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec and 68°C for 15 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Human genomic DNA was used as a template for PCR amplification.

Lane M1 : Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2 : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 11 kb fragment
Lane 2 : 13.5 kb fragment
Lane 3 : 17.6 kb fragment
Lane 4 : 21.4 kb fragment



accupower

Figure 4. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec, 65°C for40 sec, and 68°C for 20 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Lambda DNA was used as a template for PCR amplification.

Lane M1 : Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2 : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 15 kb fragment
Lane 2 : 20 kb fragment
Lane 3 : 25 kb fragment
Lane 4 : 30 kb fragment

Manual

Manual_AccuPower® ProFi Taq PCR PreMix


MSDS

MSDS_AccuPower® ProFi Taq PCR PreMix


Brochure

AccuPower® ProFi Taq PCR PreMix

All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate

카탈로그 번호 제품명 가격
K-2631 AccuPower® ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes, 20 μl reaction/tube ₩87,000
K-2632 AccuPower® ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes, 20 μl reaction/tube ₩329,000
K-2633 AccuPower® ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes, 50 μl reaction/tube ₩134,000
K-2634 AccuPower® ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes, 50 μl reaction/tube ₩502,000
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