AccuTarget™ qPCR Screening Kit

반드시 로그인 후 주문서를 작성해주시기 바랍니다.

Intercalating dye type의 Real time PCR을 이용한 유전자 screening을 통해 mRNA수준에서 gene expression level을 확인할 수 있는 pre-designed primer set 입니다.
MIQE guideline을 준수하기 위하여 정밀한 bioinformatics tools을 사용하여 design 되었습니다.
in silico 상에서 비특이적인 증폭이 발생하지 않는 것이 검증된 제품입니다.

카탈로그 번호	제품명	규격	가격
SH-0000-10	AccuTarget™ Human qPCR Screening Kit	10X	₩180,000
SH-0000-20		20X	₩250,000
SH-0000-30		30X	₩320,000
SM-0000-10	AccuTarget™ Mouse qPCR Screening Kit	10X	₩180,000
SM-0000-20		20X	₩250,000
SM-0000-30		30X	₩320,000

Species

Pathway name & Plate map

Concentration

Add/Remove Rows

제품 개요

개요

Real time PCR의 primer는 실험 결과에 큰 영향을 주기에 반드시 정밀한 디자인을 통해 실험에 활용하여야 합니다. 본 제품은 당사의 primer 및 probe design 노하우와 NCBI database RefSeq (Reference Sequence)에 등재된 최신 유전자 정보를 이용하여 표적 특이적 증폭과 낮은 dimer 형성 확률을 보이는 primer set을 디자인 후 제공해 드립니다.

AccuTarget ™ qPCR Screening kit는 각 well에 유전자 발현을 확인할 수 있는 84개 primer set가 96 well plate에 구성된 제품입니다. Control 유전자 8개까지 포함하여 총 92개의 primer set입니다.

또한 규격별(10X/20X/30X)로 96 well plate에 분주되어 있어 사용량만큼 qPCR plate에 분주하여 실험 목적에 맞게 screening용으로 사용할 수 있습니다.

Ex) PCR Plate layout

특장점

전문적인 디자인
표적 특이성, Tm value, PCR product size 등 primer design의 필수 조건을 고려하여 최적화된 primer set을 디자인합니다.
정확하고 경제적인 서비스

알고리즘과 BLAST를 통하여 표적 특이적 증폭이 확인된 primer를 제공하여 잘못 디자인된 primer set으로 소요되는 시간 및 비용을 절감할 수 있습니다.
간편한 사용

10X, 20X, 30X로 선택 주문이 가능하여 실험 목적에 맞는 사용량으로 분주하여 간편하게 사용 가능합니다.

응용 및 적용

Gene expression profile
NGS 또는 Microarray 실험 후 검증
제품 QC
Cancer Inflammation and cytokine profiling
Stem cells
Signal transduction pathways
Biomarker screening and Validation

실험 자료

AccuTarget ™ qPCR Screening kit를 이용하여 다양한 방법으로 결과 분석가능
Ex) Fold change, Scatters plot, Heatmap, etc.

제품 규격/사양

1. Pathway list

A~B	C	D~F
[0001] Adherens Junctions [0002] Adipogenesis [0003] Aging [0004] Allergy and Asthma [0005] Alzheimers Disease [0006] Amino Acid Metabolism I [0007] Amino Acid Metabolism II [0008] AMPK Signaling [0009] Androgen Receptor Signaling Targets [0010] Angiogenesis [0011] Angiogenic Growth Factor [0012] Antibacterial Response [0013] Antifungal Response [0014] Antiviral Response [0015] Apoptosis [0016] Atherosclerosis [0017] Autophagy [0018] Breast Cancer	[0019] cAMP Calcium Signaling PathwayFinder [0020] Cancer Drug Resistance [0021] Cancer Drug Target [0022] Cancer Inflammation and Immunity Crosstalk [0023] Cancer PathwayFinder [0024] Cancer Stem Cells [0025] Cardiotoxicity [0026] Cardiovascular Disease [0027] Cell Cycle [0028] Cell Death PathwayFinder [0029] Cell Junction PathwayFinder [0030] Cell Lineage Identification [0031] Cell Motility [0032] Cell Surface Markers [0033] Cellular Senescence [0034] Cellular Stress Responses [0035] Chemokines and Receptors [0036] Circadian Rhythms [0037] Common Cytokines [0038] Crohns Disease [0039] Cystic Fibrosis [0040] Cytokines and Chemokines [0041] Cytoskeleton Regulators	[0042] Dendritic and Antigen Presenting Cell [0043] Diabetes [0044] DNA Damage Signaling Pathway [0045] DNA Repair [0046] Dopamine and Serotonin Pathway [0047] Drug Metabolism [0048] Drug Metabolism Phase I Enzymes [0049] Drug Metabolism Phase II Enzymes [0050] Drug Transporters [0051] EGF PDGF Signaling Pathway [0052] Embryonic Stem Cells [0053] Endothelial Cell Biology [0054] Epigenetic Chromatin Modification Enzymes [0055] Epigenetic Chromatin Remodeling Factors [0056] Epithelial to Mesenchymal Transition [0057] Estrogen Receptor Signaling [0058] Extracellular Matrix and Adhesion Molecules [0059] Fatty Acid Metabolism [0060] Fatty Liver [0061] Female Infertility [0062] Fibrosis [0063] Focal Adhesions
G~H	I~L	M~N
[0064] GABA and Glutamate [0065] Gap Junctions [0066] Glucocorticoid Signaling [0067] Glucose Metabolism [0068] Glycosylation [0069] GPCR Signaling PathwayFinder [0070] Growth Factors [0071] Heat Shock Proteins and Chaperones [0072] Hedgehog Signaling Pathway [0073] Hematopoiesis [0074] Hepatotoxicity [0075] Hippo Signaling Pathway [0076] HIV Host Response [0077] Homeobox HOX Genes [0078] Huntingtons Disease [0079] Hypertension [0080] Hypoxia Signaling Pathway [0081] Hypoxia Signaling Pathway Plus	[0082] IL6 STAT3 Signaling Pathway [0083] IL6 STAT3 Signaling Pathway Plus [0084] Immunotoxicity [0085] Induced Pluripotent Stem Cells [0086] Inflammasomes [0087] Inflammatory Cytokines and Receptors [0088] Inflammatory Response and Autoimmunity [0089] Innate and Adaptive Immune Responses [0090] Insulin Resistance [0091] Insulin Signaling Pathway [0092] Interferons and Receptors [0093] JAK STAT Signaling Pathway [0094] let-7a Targets [0095] Leukemia [0096] Lipoprotein Signaling and Cholesterol Metabolism [0097] Liver Cancer [0098] Lung Cancer [0099] Lymphoma	[0100] Macular Degeneration [0101] Male Infertility [0102] MAP Kinase Signaling Pathway [0103] Mesenchymal Stem Cells [0104] Mitochondria [0105] Mitochondrial Energy Metabolism [0106] Molecular Toxicology PathwayFinder [0107] mTOR Signaling [0108] Multiple Sclerosis [0109] MYC Targets [0110] Necrosis [0111] Nephrotoxicity [0112] Neurogenesis [0113] Neuronal Ion Channels [0114] Neurotoxicity [0115] Neurotransmitter Receptors [0116] Neurotrophins and Receptors [0117] NFkB Signaling Pathway [0118] NFkB Signaling Pathway Plus [0119] NFkB Signaling Targets [0120] Nitric Oxide Signaling Pathway [0121] Notch Signaling Pathway [0122] Notch Signaling Pathway Plus [0123] Notch Signaling Targets [0124] Nuclear Receptors and Coregulators
O~R	S~T	U~W
[0125] Obesity [0126] Oncogenes and Tumor Suppressor Genes [0127] Osmotic Stress [0128] Osteogenesis [0129] Osteoporosis [0130] Oxidative Stress [0131] Oxidative Stress Plus [0132] p53 Signaling Pathway [0133] Pain Neuropathic and Inflammatory [0134] Parkinsons Disease [0135] Phagocytosis [0136] PI3K AKT Signaling Pathway [0137] Polycomb and Trithorax Complexes [0138] Polycomb and Trithorax Target Genes [0139] Polycystic Kidney Disease [0140] PPAR Targets [0141] Pre Eclampsia [0142] Primary Cilia [0143] Prostate Cancer [0144] Protease Activated Receptor Signaling [0145] Protein Phosphatases [0146] Retinoic Acid Signaling	[0147] Signal Transduction PathwayFinder [0148] Skeletal Muscle Myogenesis and Myopathy [0149] Stem Cell [0150] Stem Cell Signaling [0151] Stem Cell Transcription Factors [0152] Stress and Toxicity PathwayFinder [0153] Synaptic Plasticity [0154] T Cell Anergy and Immune Tolerance [0155] T Helper Cell Differentiation [0156] T Cell and B Cell Activation [0157] Telomeres and Telomerase [0158] Terminal Differentiation Markers [0159] TGFb BMP Signaling Pathway [0160] Human TGFb Signaling Targets [0161] Th1 and Th2 Responses [0162] Th17 Response [0163] Tight Junctions [0164] TNF Signaling Pathway [0165] Toll Like Receptor Signaling Pathway [0166] Transcription Factors [0167] Transplant Rejection [0168] Tumor Metastasis [0169] Type I Interferon Response [0170] Tyrosine Kinases	[0171] Ubiquitination Ubiquitinylation [0172] Unfolded Protein Response [0173] Unfolded Protein Response Plus [0174] VEGF Signaling [0175] WNT Signaling Pathway [0176] WNT Signaling Pathway Plus [0177] WNT Signaling Targets [0178] Wound Healing

◈ Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate

주문 정보

▶ 기본제품

카탈로그 번호	제품명	규격	가격
SH-0000-10	AccuTarget™ Human qPCR Screening Kit	10X	₩SH-0000-10
SH-0000-20		20X	₩SH-0000-20
SH-0000-30		30X	₩SH-0000-30
SM-0000-10	AccuTarget™ Mouse qPCR Screening Kit	10X	₩SM-0000-10
SM-0000-20		20X	₩SM-0000-20
SM-0000-30		30X	₩SM-0000-30

▶Custom 제품

카탈로그 번호	제품명	규격	가격
SPC-0001-10	AccuTarget™ qPCR Screening Kit-Pathway Custom Kit (1~5개 유전자 primer set 추가/변경 시)	10X	Inquire
SPC-0001-20		20X	Inquire
SPC-0001-30		30X	Inquire
SWC-0001-10	AccuTarget™ qPCR Screening Kit-Whole Custom Kit (Pathway 상관없이 원하는 종류로 92개(84개 primer set+8개 control)의 primer set 구성 시)	10X	Inquire
SWC-0001-20		20X	Inquire
SWC-0001-30		30X	Inquire

▶Primer set 변경/추가

Primer set 변경/추가 등을 원하시면 Custom 제품을 이용하실 수 있습니다.
기본 Pathway 번호와 제외할 well 번호 그리고 추가할 Gene ID/고유번호를 적어 order@bioneer.co.kr 로 보내주시기 바랍니다.
추가할 Gene ID는 Prdesigned siRNA 에서 검색하시어 Gene ID/고유번호를 알려주시면 됩니다.
검색이 되지 않는 유전자의 경우 Gene name 및 ID를 메일에 적어주시기 바랍니다.

Custom 제품 문의: order@bioneer.co.kr
학술 문의: qPCRarray@bioneer.com

AccuTarget™ qPCR Screening Kit

개요

특장점

응용 및 적용

실험 자료

◈ Manual

◈ Flyer

◈ MSDS

◈ Quality Assurance

▶Primer set 변경/추가