▶ 실험 자료
합성된 모든 siRNA은 MALDI-TOF mass spectrometry를 통해 품질이 엄격히 제어되며 PAGE분석을 통해 duplex structure를 확인합니다 (Figure. 1, 2).
Figure 1. MALDI-TOF mass spectrometry analysis of a custom siRNA. All siRNAs are processed by MALDI-TOF mass spectrometry to ensure its quality.
Figure 2. PAGE data of double-stranded custom siRNA. Complementary single-stranded RNA strands were hybridized to form siRNA duplex and analyzed by 15% non-denaturing PAGE.
- SS: single-stranded RNA
- DS: double-stranded siRNA
Figure 3. Knockdown efficiency of AccuTarget ™ siRNA Library. To determine the knockdown efficiency, HeLa cells were transfected with individual siRNAs at 100 nM concentration. 24 hours post-transfection, total RNA was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm: 83.8% of tested siRNAs induced > 70% knockdown and 38.1% of tested siRNAs elicited > 90% knockdown.
Figure 4. Knockdown efficiency of AccuTarget ™ Human Validated Cell Cycle siRNA set. HeLa cells were transfected with individual siRNAs targeting 33 different genes at a concentration of 20 nM. Total RNAs was isolated and the level of target mRNA was measured by qRT-PCR. This data demonstrates the effectiveness of the Turbo si-Designer algorithm.
바이오니아는 즉시 실험에 사용할 수 있도록 제작된 25,368 개의 siRNA로 이루어진 AccuTarget ™ Premade Human siRNA Library를 제공합니다. 이러한 Premade siRNA Library는 10, 20, 50또는 100 nmole Guaranteed yield 로 주문하실 수 있습니다. 다양한 Cellular Processes, 암, 질병 관련 연구목적의 25개 Pathway별 siRNA Library set를 제공합니다. 이러한 siRNA는 0.1, 0.25, 0.5, 1 nmole Guaranteed Yield 로 주문할 수 있습니다. 또한 높은 Knockdown 효능이 검증된 21개의 Pre-validated Library를 제공합니다. Pre-validated siRNA Library는 10, 20, 50, 또는 100 nmole Guaranteed Yield로 주문하실 수 있습니다.
▶ siRNA Set
Bioneer는 다양한 Cellular Processes, 암, 질병관련 연구목적의 25개 Gene Family 및 Pathway별로 siRNA Library Set를 제공합니다. 이러한 siRNA는 0.1, 0.25, 0.5, 1 nmole Guaranteed Yield 로 주문하실 수 있습니다.
| 카탈로그 번호 |
제품명 |
No. of Genes |
No. of siRNAs |
| SHS-0010 |
AccuTarget ™ Human Antioxidant siRNA set |
38 |
114 |
| SHS-0020 |
AccuTarget ™ Human Apoptosis siRNA set |
270 |
810 |
| SHS-0250 |
AccuTarget ™ Human Cancer siRNA set |
1,114 |
3,342 |
| SHS-0030 |
AccuTarget ™ Human Caspase siRNA set |
33 |
99 |
| SHS-0040 |
AccuTarget ™ Human Cell cycle siRNA set |
107 |
321 |
| SHS-0050 |
AccuTarget ™ Human Cyclase siRNA set |
21 |
63 |
| SHS-0060 |
AccuTarget ™ Human Cytochrome P450 siRNA set |
50 |
150 |
| SHS-0070 |
AccuTarget ™ Human Deaminase siRNA set |
22 |
66 |
| SHS-0080 |
AccuTarget ™ Human GPCR signaling pathway siRNA set |
699 |
2,097 |
| SHS-0090 |
AccuTarget ™ Human Helicase siRNA set |
108 |
342 |
| SHS-0100 |
AccuTarget ™ Human Isomerase siRNA set |
97 |
291 |
| SHS-0110 |
AccuTarget ™ Human Kinase siRNA set |
670 |
2,010 |
| SHS-0120 |
AccuTarget ™ Human Ligase siRNA set |
261 |
783 |
| SHS-0130 |
AccuTarget ™ Human Lyase siRNA set |
115 |
345 |
| SHS-0140 |
AccuTarget ™ Human Motor siRNA set |
120 |
360 |
| SHS-0150 |
AccuTarget ™ Human NF-kB pathway siRNA set |
36 |
108 |
| SHS-0160 |
AccuTarget ™ Human Nucleic acid binding siRNA set |
2,471 |
7,413 |
| SHS-0170 |
AccuTarget ™ Human Oxidoreductase siRNA set |
534 |
1,602 |
| SHS-0180 |
AccuTarget ™ Human Peptidase siRNA set |
470 |
1,410 |
| SHS-0190 |
AccuTarget ™ Human Phosphatase siRNA set |
183 |
549 |
| SHS-0200 |
AccuTarget ™ Human Receptor siRNA set |
1,468 |
4,404 |
| SHS-0210 |
AccuTarget ™ Human Transferase siRNA set |
1,347 |
4,122 |
| SHS-0220 |
AccuTarget ™ Human Transporter siRNA set |
988 |
2,964 |
| SHS-0230 |
AccuTarget ™ Human Tubulin siRNA set |
18 |
54 |
| SHS-0240 |
AccuTarget ™ Human Ubiquitin siRNA set |
73 |
219 |
▶ siRNA Subset
siRNA subset library는 Kinase 또는 Phosphatase library의 functional family subset입니다 .이러한 siRNA는 0.1, 0.25, 0.5, 1 nmole guaranteed yield 로 주문하실 수 있습니다.
▶ siRNA Druggable Set
0.1, 0.25, 0.5, 1 nmole Guaranteed Yield 로 주문하실 수 있습니다.
