AccuNanoBead™ NH2 Magnetic Nanobeads, size 400 nm (0.5 g)

Magnetic Beads는 세포실험이나 DNA정제에서부터 질병의 관찰을 위한 소재로도 사용되고, 또한 직접적인 치료를 위한 소재로도 사용이 가능하다. 본 AccuNanoBead는 크기가 나노이기 때문에 상대적으로 표면적이 넓어서, 높은 Binding Capacity를 보이는 장점이 있다.
₩280,000
카탈로그 번호
TA-1011-1



제품 개요

최근에 Magnetic Beads를 DNA 및 RNA 추출하는데 많이 사용되고 있는데, Magnetic Beads는 DNA/RNA 정제에서부터 질병의 진단을 위한 소재로 사용되고 있다. Magnetic Beads의 원리는 Magnetic Beads 표면에 있는 하이드록시 기능기가DNA 및 RNA와 결합하게 한 후에 외부 자기장(Magnetic field)을 이용하여 Magnetic Beads를 원하는 위치로 이동하거나 부착하여 정제(purification)를 하게 하는 것이다. 또한 기존의 Magnetic Beads가 마이크로 크기인데 반하여, 본 AccuNanoBead는 크기가 나노이기 때문에 상대적으로 표면적이 넓어서, 높은 Binding Capacity를 보이는 장점이 있다.

Magnetic Nanobeads의 특징

  • 평균 자성입자 크기는 400 nm
  • 입자크기분포의 균일성
  • 구형의 실리카 코팅 자성입자
  • DNA/RNA 정제에 우수한 효율성
  • 신속한 DNA/RNA 정제  


Magnetic Nanobeads의 응용분야

  • 자성체를 이용한 DNA/RNA 분리
  • 자성체를 이용한 단백질 정제
  • 자성체를 이용한 바이오시밀러 분리
  • 자성체를 이용한 항체 분리


Applications

 Magnetic Protein Purification
 Magnetic Biosimilar Separation
 Magnetic Antibody Separation


FE-SEM and TEM Images of Magnetic Nanobeads

Figure 1

Figure 1. FE-SEM image of Magnetic Nanobeads; SEM images show that Magnetic Nanobeads are sphere shape.


EDS Analysis of Magnetic Nanobeads

Figure 2

Figure 2. EDS analysis result of Magnetic Nanobeads; EDS shows that Magnetic Nanobeads are composed of silica and Iron oxide.


Size Distribution of Magnetic Nanobeads

Figure 3

Figure 3. Size distribution diagram of silica magnetic nanobeads. The average size of magnetic beads is approximately 400 nm.


Protein Purification Method by using Magnetic Nanobeads

First, load the protein sample to tube and then put Magnetic Nanobeads to combine protein sample together with Magnetic Nanobeads. Fix Magnetic Nanobeads with external Magnetic field to wash the solution and then elute protein from Magnetic Nanobeads. Using Magnetic Nanobeads to use in purification is much faster to separate protein compare to an existing method.

Figure 4

Figure 4. Recombinant Protein and Antibody protein purification protocol by Magnetic Nanobeads.


Comparison of Protein Purification Beads

Figure 5

Figure 5. (a) SEM image of 400 nm Bioneer magnetic nanobeads, (b) SEM image of A company magnetic beads, (c) SEM image of B company magnetic beads, (d) SEM image of C company magnetic beads, (e) SEM image of D company magnetic beads Figure 5 shows scanning electron microscope images of Bioneer magnetic nanobeads and different company magnetic beads(b~e). Scanning electron microscope image of synthesized magnetic nanobeads shows that magnetic nanobeads of Bioneer is 400 nm size and spherical shape. And different company magnetic beads are micro meter size and various shape.


Comparison of Protein Purification Yield

The protein purification yields of Bioneer Ni-NTA magnetic nanobeads and other company magnetic beads are listed in Table 1. These results indicate that the purification yield of Bioneer Magnetic beads is higher than that of four different company magnetic beads. In addition, purity of protein purification was more than 90%. His-Tagged DNA Polymerase protein of the 97 kDa expressed in E. coli was used in experiment. Purification yield is amount of purified target protein from loading protein. Bioneer magnetic nanobeads represents the highest purification yield compared with that of four different company magnetic beads because the surface area of magnetic beads is increased due to the size effect.

Table 1

Table 1. Comparison of protein purification yield of bioneer beads and other company beads.


Figure 6

Figure 6. Comparison of elution protein of bioneer beads and other company beads.


Figure 7

Figure 7. SDS-PAGE image of purified 94 kDa DNA Polymerase with Bioneer magnetic Nanobeads.

[Ordering Information]

Magnetic Nanobeads는 다양한 기능기를 코팅하여 연구용 및 분리용으로 판매합니다.

 

Cat No.

제품명

규격

가격(VAT별도)

TA-1010-1

AccuNanoBeadTM Silica Magnetic Nanobeads, size 400nm

0.5 g/25 ml

250,000원

TA-1010-2

AccuNanoBeadTM Silica Magnetic Nanobeads, size 400 nm

1.0 g/50 ml

450,000원

TA-1010-3

AccuNanoBeadTM Silica Magnetic Nanobeads, size 400 nm

10 g/500 ml

2,550,000원

TA-1011-1

AccuNanoBeadTM NH2 Magnetic Nanobeads, size 400 nm

0.5 g

280,000원

TA-1012-1

AccuNanoBeadTM COOH Magnetic Nanobeads, size 400 nm

0.5 g

290,000원

TA-1013-1

AccuNanoBeadTM Epoxy Magnetic Nanobeads, size 400 nm

0.5 g

310,000원

TA-1014-1

AccuNanoBeadTM C18 Magnetic Nanobeads, size 400 nm

0.5 g

310,000원

TA-1015-1

AccuNanoBeadTM Streptavidin Magnetic Nanobeads, size 400 nm

50 mg/25 ml

250,000원

TA-1016-1

AccuNanoBeadTM Biotin Magnetic Nanobeads, size 400 nm

0.5 g/25 ml

310,000원

TA-1017-1

AccuNanoBeadTM Ni-NTA Magnetic Nanobeads, size 400 nm

0.5 g/25 ml

510,000원

TA-1019-1

AccuNanoBeadTM Thiol Magnetic Nanobeads, size 400 nm

0.5 g

380,000원

TA-1021-1

AccuNanoBeadTM Protein G Magnetic Nanobeads, size 400nm

40mg/1ml

152,000원

TA-1021-2

AccuNanoBeadTM Protein G Magnetic Nanobeads, size 400nm

200mg/10ml

522,000원

TA-1021-3

AccuNanoBeadTM Protein G Magnetic Nanobeads, size 400nm

500mg/25ml

990,000원

TA-1022-1

AccuNanoBeadTM Protein A Magnetic NanoBeads, size 400 nm

40mg/1mL

152,000원

TA-1022-2

AccuNanoBeadTM Protein A Magnetic NanoBeads, size 400 nm

200mg/10mL

522,000원

TA-1022-3

AccuNanoBeadTM Protein A Magnetic NanoBeads, size 400 nm

500mg/25mL

990,000원

TS-1000-1

Magnet Φ15x1.5 and Screw Tube 1.5 ml

1 set

5,000원

*대량 주문은 별도 문의바랍니다.



관련 제품

Cat No.

제품명

규격

가격(VAT별도)

K-7250

AccuRapid™ Cell-Free Protein Expression Kit

45 ul x 24 reactions

450,000원

K-7300

ExiProgen™ EC Protein Synthesis Kit

16 reactions

590,000원

K-7301

ExiProgen™ EC Protein Synthesis Kit

32 reactions

990,000원

K-7302

ExiProgen™ EC Protein Synthesis Kit

96 reactions

2,450,000원

D-2010

AccuLadder™ Protein Size Marker (Broad)

500 ul

120,000원

D-2020

AccuLadder™ Protein Size Marker (Low)

500 ul

70,000원

K-3030

AccuPrep® Plasmid Extraction kit

200 prep

120,000원

K-3034

AccuPrep® PCR purification Kit

200 prep

135,000원

K-7210

ExiPrep™ His-tagged Protein Purification Kit

96 reactions

500,000원

K-7200

AccuPrep™ His-tagged Protein Purification Kit

-

210,000원

 

 

기술지원 및 주문

제품에 대한 자세한 정보는 다음 email로 문의 바랍니다. nano-support@bioneer.com

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