AccuPower® Taq PCR PreMix & Master Mix

AccuPower® Taq PCR PreMix 는 바이오니아의 특허기술로 Taq DNA polymerase, dNTPs, reaction buffer 등 PCR 수행에 필요한 구성성분을 혼합하여 1회 분량씩 진공 건조시킨 제품입니다. 또한 안정화 물질이 첨가되어 있어 상온 (25°C) 보관할 경우에는 한 달 동안, 냉동 보관할 경우에는 2년 동안 활성이 안정적으로 유지됩니다.

₩54,000
카탈로그 번호
K-2601-CFG

특장점

  • 안정성

    AccuPower® Taq PCR PreMix 는 PCR 반응 혼합액에 안정화 물질이 포함되어 있어 상온배송이 가능하고 solution type 제품보다 안정성이 뛰어납니다.

  • 민감도

    미량의 DNA 도 뛰어난 민감도와 증폭 효율로 PCR 반응이 가능합니다.

  • 편리성

    각 PCR tube에 1회 반응씩 PCR 반응물이 건조되어 있어 누구나 손쉽게 template DNA 및 primer 만을 첨가하여 PCR 반응을 수행할 수 있습니다.

  • 재현성

    ISO 9001 품질 시스템 하에서 대량 생산되어 lot 간 편차를 최소화 하였으며, 반복적인 실험에도 재현성 있는 실험 결과를 제공합니다.

응용 및 적용

• Conventional PCR

• Primer extension

• TA cloning

• Gene sequencing

실험 자료

premix figure1

Figure. Comparison of PCR amplification efficiency between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix.
Target Gene: human insulin receptor gene. The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.

Lane M: 100 bp DNA Ladder (Bioneer, Cat. No. D-1030)
Lane 1: 10 ng human genomic DNA
Lane 2: 1 ng human genomic DNA
Lane 3: 100 pg human genomic DNA
Lane 4: 10 pg human genomic DNA

제품 규격/사양

Enzyme

Taq DNA polymerase

5'-> 3' exonuclease activity

Yes

3'-> 5' exonuclease activity

No

3'- A overhang

Yes

Fragment size

~ 10 kb

실험 자료

premix figure1

Figure 1. Comparison of PCR amplification efficiency between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix
Target Gene: IRGC (Immunity-related GTPase family, cinema) The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 20 sec, 55°C for 20 sec, and 72°C for 30 sec. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.

Lane M: 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1: 10 ng human genomic DNA
Lane 2: 1 ng human genomic DNA
Lane 3: 100 pg human genomic DNA
Lane 4: 10 pg human genomic DNA 


premix figure1

Figure 2. Comparison of PCR amplification of long targets between AccuPower® Taq PCR PreMix from Bioneer and other suppliers' PCR master mix
The cycling conditions for AccuPower® Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec, and 68°C for 8 min. PCR reactions using other suppliers' PCR master mix were performed according to each supplier's protocol.

Lane M: 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1: 3 kb fragment (human tumor protein p53 gene)
Lane 2: 4 kb fragment (human beta globin region)
Lane 3: 4.5 kb fragment (human DNA cross-link repair 1A gene)
Lane 4: 8 kb fragment (human hemoglobin epsilon 1 gene)

Manual

Manual_AccuPower® Taq PCR PreMix

Manual_AccuPower® Taq PCR Master Mix

MSDS

MSDS_AccuPower® Taq PCR PreMix

Brochure

AccuPower® Taq Series

All about PCR

Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

ISO 9001 - certificate

ISO 13485 - certificate

카탈로그 번호 제품명 가격
K-2601 AccuPower® Taq PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached caps / 96 tubes, 20 µl reaction/tube ₩54,000
K-2602 AccuPower® Taq PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached caps / 480 tubes, 20 µl reaction/tube ₩199,000
K-2603 AccuPower® Taq PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached caps / 96 tubes, 50 µl reaction/tube ₩81,000
K-2604 AccuPower® Taq PCR PreMix, 0.2 ml thin-wall 8-strip tubes with attached caps / 480 tubes, 50 µl reaction/tube ₩304,000
K-2609 Taq PCR MasterMix, 2.5 ml of 2X Master mix solution ₩97,000
K-2610 Taq PCR MasterMix, 25 ml of 2X Master mix solution 12.5 ml * 2 tubes ₩632,000
Copyright © 2018 BIONEER CORPORATION. All rights reserved.