MagListo™ 5M Plasmid Extraction Kit

MagListo™ 5M Plasmid Extraction Kit은 Magnetic Nanobead와 MagListo™ Magnetic Separation Rack을 이용하여 5분 (mini), 10분 (midi), 15분 (maxi) 내에 배양된 박테리아 세포로부터 plasmid DNA를 추출할 수 있는 제품입니다. 자성분리방식으로 세포 분쇄물과 plasmid DNA를 분리하고 농축 및 정제하는 과정을 거치기 때문에 원심분리방법에 비해 빠르고 간편하게 plasmid DNA를 분리할 수 있습니다. 본 제품은 한 kit를 사용하여 mini, midi, maxi scale로 prep할 수 있어 별도의 kit를 구매할 필요가 없으며, midi나 maxi prep을 위한 vacuum system이나 air pressure system을 구비할 필요가 없어 실험비용을 절감할 수 있습니다.

₩99,000
카탈로그 번호
K-3601-CFG

Features and Benefits

  • Magnetic nano bead를 이용하여 Mini-5분, Midi-10분, Maxi-15분 만에 plasmid 추출
  • Mini, midi, maxi prep kit을 따로 구매할 필요 없이 하나의 kit로 해결
  • 본 extraction kit와 별도의 MagListo 외에 고가의 추가 장비 불필요
  • MagListo 하나로 plasmid prep 외에 여러 가지 DNA 및 RNA 추출 및 단백질 정제까지 가능

Reagents K-3601 K-3600
Buffer ① (Resuspension buffer) 25 ml x 1 ea 110 ml x 1 ea
Buffer ② (Lysis buffer) 25 ml x 1 ea 110 ml x 1 ea
Buffer ③ (Neutralization buffer) 25 ml x 1 ea 110 ml x 1 ea
Buffer ④ (Binding buffer) 75 ml x 1 ea 160 ml x 2 ea
Buffer ⑤ (Washing buffer) 120 ml x 1 ea 250 ml x 2 ea
Buffer ⑥ (Elution) 15 ml x 1 ea 50 ml x 1 ea
RNase A powder, lyophilized 3 mg x 1 ea 12 mg x 1 ea
Magnetic nano bead 1.2 ml x 5 ea 1.2 ml x 25 ea
  *mini – 100 rxn, midi – 10 rxn, maxi – 4 rxn   **mini – 500 rxn, midi – 50 rxn, maxi – 18 rxn

Specifications

  8-channel scale mini scale midi scale maxi scale
Starting culture volume 1 - 1.5 ml 1 - 10 ml 20 - 50 ml 100 - 200 ml
Preparation time < 5 min < 5 min < 10 min < 15 min
Elution volume 100 μl 100 μl 500 μl 1 ml
Expected DNA yield Up to 10 μg Up to 20 μg Up to 200 μg Up to 500 μg
Expected DNA purity A260/280 > 1.8

Applications

Gene cloning
PCR
Real-time PCR
Sequencing
Transformation
Transfection
in-vitro transcription / translation


Experimental Data

Figure 1. Comparison of plasmid DNA purified with MagListo™ 5M Plasmid Extraction Kit and competitors' products (magnetic bead Type).

M1; Bioneer 100 bp ladder
1; 2 kb plasmid DNA purified with Bioneer MagListoTM 5M Plasmid Extraction Kit
2; 2 kb plasmid DNA purified with competitor P kit
3; 2 kb plasmid DNA purified with competitor I kit
M2; Bioneer 1 kb ladder


Sample ID Nucleic Acid Conc. 260/280 260/230
Bioneer 117.9 ng/µl 2.03 1.91
Competitor P 95.9 ng/µl 2.05 1.91
Competitor I 90.9 ng/µl 2.03 1.9



Figure 2. Electrophoresis data of three scale plasmid DNA extraction protocol (mini, midi, maxi) and restriction endonuclease treated (500 ng quantified).


M1; Bioneer 100 bp ladder
1; Plasmid DNA purified with mini scale protocol (1)
2; Plasmid DNA purified with mini scale protocol/digested by EcoRI (1)
3; Plasmid DNA purified with mini scale protocol (2)
4; Plasmid DNA purified with mini scale protocol/digested by EcoRI (2)
5; Plasmid DNA purified with midi scale protocol (1)
6; Plasmid DNA purified with midi scale protocol/digested by EcoRI (1)
7; Plasmid DNA purified with midi scale protocol (2)
8; Plasmid DNA purified with midi scale protocol/digested by EcoRI (2)
9; Plasmid DNA purified with maxi scale protocol
10; Plasmid DNA purified with miaxi scale protocol/digested by EcoRI
M2; Bioneer 1 kb ladder



Figure 3. Electrophoresis data of 500 ng of several size plasmids purified with MagListo™ 5M Plasmid Extraction Kit.


M1; Bioneer 100 bp ladder
1; 3.5 kb plasmid DNA
2; 5.4 kb plasmid DNA
3; 8 kb plasmid DNA
M2; Bioneer 1 kb ladder



Figure 4. High quality sequencing data of pGEM-B1 clones purified with MagListo™ 5M Plasmid Extraction Kit.


Manual

Manual_MagListo™ 5M Plasmid DNA Extraction Kit
MagListo™ 5M Plasmid Extraction Kit-2014 Quick Protocol


MSDS

• MSDS_MagListo™ 5M Plasmid Extraction Kit


Brochure

MagListo™ nucleic acid extraction kit series


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 - certificate










Cat. No. Product Description Price
K-3600 MagListo™ 5M Plasmid Extraction Kit, 500 rxn in mini / kit ₩347,000
K-3601 MagListo™ 5M Plasmid Extraction Kit, 100 rxn in mini / kit ₩99,000

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50050 50 ml tube, 500 ea / pk ₩107,800
HT-15-NG Graduated 1.5 ml Microcentrifuge Tubes, Natural, 500 ea ₩19,200
HT-20-NG Standard 2.0 ml Microcentrifuge Tubes, Natural, 500 ea ₩21,600
TM-1000 MagListo™-8 Ch Magnetic Separation Rack, 1 ml tube x 8 holes ₩255,000
TM-1010 MagListo™-2 Magnetic Separation Rack, 2 ml tube x 8 holes ₩255,000
TM-1020 MagListo™-15 Magnetic Separation Rack, 15 ml tube x 6 holes ₩455,000
TM-1030 MagListo™-50 Magnetic Separation Rack, 50 ml tube x 3 holes ₩455,000



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