MagListo™ Protein G Kit (20 reactions)

MagListo™ Protein G Kit는 자성분리방식으로 항체를 빠르고 간편하게 정제할 수 있는 제품으로, Protein G Magnetic Nanobead와 항체 정제에 필요한 buffer로 구성되어 있습니다. Magnetic Nanobead는 고순도(> 95%) protein G가 코팅된 silica bead를 사용하고 있으며 silica bead안에는 magnetic particles이 포함되어 있습니다. 이러한 bead는 특이적으로 항체를 인지하여 항체정제, immunoprecipitation, 항원-항체 상호작용 연구, 단백질 복합체 연구, cell separation 등에 응용할 수 있습니다.

₩210,000
In stock
SKU
K-7710

Features and Benefits

  • Fast Binding: 강력한 자성력은 bead의 빠른 회수율로 실험을 보다 빠르고 손실 없이 진행할 수 있습니다. 
  • Large Surface Area: 평균직경 400 nm의 magnetic nanobead는 표면적이 넓고 결합력이 우수하여 높은 binding capacity를 보장합니다.
  • Specificity:균일한 구형의 nanobead로 비특이적 결합을 감소시켰습니다.

Kit Components

    Kit components Cat. No. K-7710
    AccuNanoBead™ Protein G Magnetic NanoBeads 1 ml x 1 ea (4%)
    Binding & Washing Buffer 20 ml x 2 ea
    Elution Buffer 1 ml
    Neutralization Buffer 1 ml
    Manual 1 ea

    Specifications

    Feature Specification
    Matrix Silica-coated Fe3O4
    Concentration 40 mg/ml (4% suspension)
    Max. Binding Capacity > 30 μg Human IgG/mg beads sample
    Storage and Stability Beads can be stored at 2~8°C until the expiration date. Do not freeze and vortex vigorously.

    Application

    Antibody purification, Antigen purification, Immunoprecipitation, Protein-protein interaction, and Cell separation etc.

    Experimental Data



    Figure 1. Comparison of protein binding efficiency of AccuNanoBead™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit with a competitor.
    A) Elution fraction contains Human IgG purified with Protein G magnetic bead from Bioneer(MagListo™), a competitor L or a competitor Q. Upper and lower bands are heavy (HC) and light chain (LC) of purified Human IgG in SDS-PAGE gel, respectively.
    B) Comparison of binding capacity of Bioneer’s MagListo™ and competitor’s. Amount of bound IgG is quantified by UV-spectrophotometer. All reactions are carried out for 1 hour at room temperature. Human IgG is purchased from Sigma (Cat. no. I4506). M: protein marker (Bioneer, Cat. No. D-2020), Bioneer: MagListo™ Protein G Kit.


    Figure 2. Purification of Human IgG with AccuNanoBead™ Protein G Magnetic NanoBeads in MagListo™ Protein G Kit.
    IgG was purified from human whole blood. Arrows point to heavy and light chains of IgG, respectively.
    M: protein marker, W: human whole blood, U: unbound fraction, E1 & 2: elution fraction 1 & 2.


    Cat. No. Product Description Price
    K-7710 MagListo™ Protein G Kit, 1 x 1 mL ₩210,000

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