AccuPower® GoldHotstart Taq PCR PreMix & Master Mix

AccuPower® GoldHotStart Taq PCR PreMix는 GoldHotStart Taq DNA Polymerase를 적용하여 반응 특이성과 PCR 증폭효율을 높인 제품으로, Taq DNA polymerase가 낮은 온도에서 반응하면서 발생할 수 있는 mis-priming, primer-dimer와 같은 비특이 반응을 줄여줍니다. 그리고 DNA polymerase, dNTPs, reaction buffer 등 PCR 수행에 필요한 구성성분이 혼합되어 1회 분량씩 진공 건조되어 있습니다.

₩69,900
카탈로그 번호
K-2621-CFG

Features and Benefits

Specificity:
GoldHotStart Taq DNA polymerase는 반응 전(zero cycle)의 비특이 증폭산물의 생성을 최소화합니다. PCR 온도가 증가하면서 활성화된 HotStart Taq polymerase는 정확한 target만을 빠르게 증폭합니다.

Sensitivity:
뛰어난 민감도와 증폭효율로 미량의 human gDNA target을 검출합니다. 타사 제품과의 비교 실험을 통해 우수한 민감도를 확인했습니다.

Ease-of-Use:
각각의 PCR tube에 DNA polymerase와 PCR 수행에 필요한 모든 구성 성분이 포함되어 있어 template DNA, primer set, D.W.만 넣어 바로 PCR 반응을 수행할 수 있습니다. 또한 전기 영동 시에 필요한 tracking dye와 침강제가 포함되어 있어 sample loading buffer를 추가로 넣을 필요가 없으므로 간편하게 사용할 수 있습니다. 

Stability:
PCR 반응 혼합물에 안정화제를 첨가하여 건조시켜, 장기간 보관하더라도 활성을 안정적으로 유지합니다.

Reproducibility:
ISO 9001 품질 시스템 하에서 one-batch system으로 대량 생산되어 각 batch에 대한 철저한 QC를 거친 후 균일한 품질의 제품으로 공급되기 때문에 사용자가 대량의 시료를 반복적으로 처리할 때 각 tube마다 발생하는 편차 문제를 해결하여 재현성있는 실험 결과를 제공합니다.

Specifications

Enzyme: GoldHotStart Taq polymerase
5' to 3' exonuclease activity : Yes
3' to 5' exonuclease activity : No
3' – A Overhang : Yes
PCR product size : ~ 5 kb (human)


Applications

High specificity PCR
High sensitivity PCR
gDNA template PCR
Low-copy target PCR
Multiple primer pairs PCR
cDNA template PCR
TA cloning


Experimental data

AccuPower GoldHotstart Taq PCR PreMix
Figure1. Comparison of PCR amplification efficiency between AccuPower® GoldHotStart Taq PCR PreMix from Bioneer and other suppliers’ Hotstart PCR master mix.
The cycling conditions for AccuPower® GoldHotStart Taq PCR PreMix were 95°C for 5 min, 30 cycles of 95°C for 30 sec, 55°C for 30 sec and 72°C for 30 sec. PCR reactions using other suppliers’ PCR master mix were performed according to each supplier's protocol.
Target : human insulin receptor gene.

Lane M; 100 bp DNA Ladder(Cat. No. D-1030, Bioneer)
Lane 1; 10 ng of human genomic DNA
Lane 2; 1 ng of human genomic DNA
Lane 3; 100 pg of human genomic DNA
Lane 4; 10 pg of human genomic DNA


AccuPower GoldHotstart Taq PCR PreMix
Figure 2. Comparison of PCR amplification specificity between AccuPower® GoldHotStart Taq PCR PreMix from Bioneer and other suppliers’ Hot start PCR master mix.
The cycling conditions for AccuPower® GoldHotStart Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 30 sec, 57°C for 30 sec and 72°C for 30 sec. PCR reactions using other suppliers’ PCR master mix were performed according to each supplier's protocol.
Target : ApoE gene (The PCR product size is 268 bp).

Lane M; 100 bp DNA Ladder (Cat. No. D-1030, Bioneer)
Lane 1,2; 100 ng human genomic DNA
Lane 3,4; 10 ng human genomic DNA

AccuPower GoldHotstart Taq PCR PreMix

Figure 3. Comparison of PCR amplification specificity between AccuPower® GoldHotStart Taq PCR PreMix from Bioneer and other suppliers’ Hotstart PCR master mix.
The cycling conditions for AccuPower® GoldHotStart Taq PCR PreMix were 95°C for 5 min, 35 cycles of 95°C for 30 sec, 65°C for 30 sec and 72°C for 30 sec. PCR reactions using other suppliers’ PCR master mix were performed according to each supplier's protocol.

Lane M; 100 bp DNA Ladder (Cat. No. D-1020, Bioneer)
Lane 1; 100 ng human genomic DNA
Lane 2; 10 ng human genomic DNA

Manual

  • AccuPower®GoldHotstart Taq PCR PreMix

  • AccuPower®GoldHotstart TaqPCR MasterMix

MSDS

  • MSDS_AccuPower®GoldHotstart Taq PCR PreMix


Brochure

• All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

  • ISO 9001 – certificate









Cat. No. Product Description Price
K-2621 AccuPower® GoldHotstart Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes, 20 μl reaction/tube ₩69,900
K-2622 AccuPower® GoldHotstart Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes, 20 μl reaction/tube ₩262,200
K-2623 AccuPower® GoldHotstart Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes, 50 μl reaction/tube ₩104,900
K-2624 AccuPower® GoldHotstart Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes, 50 μl reaction/tube ₩393,200
K-2629 AccuPower® GoldHotstart Taq PCR MasterMix, 2.5 ml of 2X Master mix solution ₩124,000
K-2630 AccuPower® GoldHotstart Taq PCR MasterMix, 25 ml of 2X Master mix solution ₩818,000


























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