AccuPower® ProFi Taq PCR PreMix

AccuPower® ProFi Taq PCR PreMix는 Bioneer 에서 개발한 ProFi Taq DNA polymerase을 사용하여 높은 정확성과 증폭효율로 긴 template DNA를 정확하게 증폭할 수 있는 제품입니다. ProFi Taq DNA polymerase와 dNTPs, reaction buffer 등 PCR 수행에 필요한 구성성분이 혼합되어 1회 분량씩 진공 건조되어 있어, template DNA 와 primers의 첨가만으로 간편하게 high fidelity를 갖는 long PCR product를 얻을 수 있습니다. 

₩83,000
카탈로그 번호
K-2631-CFG

Features and Benefits

Sensitivity :
뛰어난 민감도와 증폭효율로 미량의 human gDNA target을 검출합니다. 타사 제품과의 비교 실험을 통해 우수한 민감도를 확인했습니다.

Long Range PCR:
Lambda DNA의 경우 30 kb, human gDNA의 경우 21 kb의 PCR 산물을 효과적으로 증폭할 수 있습니다.

Ease-of-use:
각각의 PCR tube에 ProFi Taq DNA polymerase와 PCR 반응에 필요한 모든 구성 성분이 포함되어 있어 template DNA, primer set 와 D.W.만 넣어 바로 PCR 반응을 수행할 수 있습니다. 또한 전기영동 시에 필요한 tracking dye와 침강제가 포함되어 있어 별도로 sample loading buffer를 첨가할 필요가 없으므로 간편하게 사용할 수 있습니다. 

Stability:
PCR 반응 혼합물에 안정화제를 첨가하여 건조시켜, 장기간 보관하더라도 활성을 안정적으로 유지합니다. 

Reproducibility:
ISO 9001 품질 시스템 하에서 one-batch system으로 대량 생산되어 각 batch에 대한 철저한 QC를 거친 후 균일한 품질의 제품으로 공급되기 때문에 사용자가 대량의 시료를 반복적으로 처리할 때 각 tube마다 발생하는 편차 문제를 해결하여 재현성있는 실험 결과를 제공합니다.

Specifications

5' to 3' exonuclease activity : Yes
3' to 5' exonuclease activity : Yes
3' – A Overhang : Yes
PCR product size : ~ 30 kb

Application

- Primer extension
- long-range amplification from genomic DNA
- High amplification efficiency
- Excellent performance on difficult templates
- Amplification of low-copy targets
- High yield and high sensitivity PCR

Experimental data

accupower
Figure 1. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase.
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5min, 30 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30sec. PCR reaction using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target : human Insulin receptor gene.
Lane M : 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human genomic DNA
Lane 2 : 1 ng of human genomic DNA
Lane 3 : 100 pg of human genomic DNA
Lane 4 : 10 pg of human genomic DNA


accupower
Figure2. Comparison of PCR amplification efficiency between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
cDNA synthesized from 10-fold serial-diluted human total RNA from 10 ng to 10 pg using AccuPower® RocketScript™ Cycle RT PreMix.(Bioneer, Cat. No K-2201) wase used as a template for PCR amplification. The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5min, 33 cycles of 95°C for 20 sec, 55°C for 20 sec and 72°C for 30 sec. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Target : human GAPDH gene.
Lane M : 100 bp DNA Ladder(Bioneer, Cat. No. D-1030)
Lane 1 : 10 ng of human total cDNA
Lane 2 : 1 ng of human total cDNA
Lane 3 : 100 pg of human total cDNA
Lane 4 : 10 pg of human total cDNA


accupower
Figure 3 . Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 30 cycles of 95°C for 20 sec, 65°C for 20 sec and 68°C for 4 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol.

Lane M : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 2 kb fragment (human tumor protein p53 gene)
Lane 2 : 3 kb fragment (human tumor protein p53 gene)
Lane 3 : 4.5kb fragment (human DNA cross-link repair 1A gene)
Lane 4 : 8 kb fragment (human hemoglobin epsilon 1 gene


accupower
Figure 4. Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec and 68°C for 15 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Human genomic DNA was used as a template for PCR amplification.

Lane M1 : Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2 : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 11 kb fragment
Lane 2 : 13.5 kb fragment
Lane 3 : 17.6 kb fragment
Lane 4 : 21.4 kb fragment


accupower
Figure 5 . Comparison of PCR amplification of long targets between ProFi Taq DNA polymerase from Bioneer and other suppliers' DNA polymerase
The cycling conditions for ProFi Taq DNA polymerase were 95°C for 5 min, 32 cycles of 95°C for 20 sec, 65°C for40 sec, and 68°C for 20 min. PCR reactions using other suppliers' DNA polymerase were performed according to each supplier's protocol. Lambda DNA was used as a template for PCR amplification.

Lane M1 : Lambda/Hind III marker (Bioneer, Cat. No. D-1050)
Lane M2 : 1 kb DNA Ladder (Bioneer, Cat. No. D-1040)
Lane 1 : 15 kb fragment
Lane 2 : 20 kb fragment
Lane 3 : 25 kb fragment
Lane 4 : 30 kb fragment 


Manual

• Manual_AccuPower® ProFi Taq PCR PreMix



MSDS

MSDS_AccuPower® ProFi Taq PCR PreMix


Brochure

AccuPower® ProFi Taq PCR PreMix
• All about PCR


Quality Assurance

Bioneer is the holder of Quality Management System Certificates for the following standards.

• ISO 9001 – certificate

• EN ISO 13485 - certificate








Cat. No. Product Description Price
K-2631 AccuPower® ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes, 20 μl reaction/tube ₩83,000
K-2632 AccuPower® ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes, 20 μl reaction/tube ₩313,000
K-2633 AccuPower® ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 96 tubes, 50 μl reaction/tube ₩128,000
K-2634 AccuPower® ProFi Taq PCR PreMix, 0.2 ml thin-wall 8-tube strips with attached caps / 480 tubes, 50 μl reaction/tube ₩478,000












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